Genome-wide localization of the polyphenol quercetin in human monocytes
Abstract Background Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-07-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5966-9 |
| _version_ | 1818246394374258688 |
|---|---|
| author | Dana Atrahimovich Avraham O. Samson Yifthah Barsheshet Jacob Vaya Soliman Khatib Eli Reuveni |
| author_facet | Dana Atrahimovich Avraham O. Samson Yifthah Barsheshet Jacob Vaya Soliman Khatib Eli Reuveni |
| author_sort | Dana Atrahimovich |
| collection | DOAJ |
| description | Abstract Background Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given to its mechanism of action at the genome-wide level. The present study aims to characterize quercetin’s interaction with cellular DNA and to show its subsequent effect on downstream transcription. Results Two massive parallel DNA-sequencing technologies were used: Chem-seq and RNA-seq. We demonstrate that upon binding to DNA or genome-associated proteins, quercetin acts as a cis-regulatory transcription factor for the expression of genes that are involved in the cell cycle, differentiation and development. Conclusions Such findings could provide new and important insights into the mechanisms by which the dietary polyphenol quercetin influences cellular functions. |
| first_indexed | 2024-12-12T14:48:07Z |
| format | Article |
| id | doaj.art-17b8b1aa419143df994052e552020fb8 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-12T14:48:07Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-17b8b1aa419143df994052e552020fb82022-12-22T00:21:04ZengBMCBMC Genomics1471-21642019-07-012011910.1186/s12864-019-5966-9Genome-wide localization of the polyphenol quercetin in human monocytesDana Atrahimovich0Avraham O. Samson1Yifthah Barsheshet2Jacob Vaya3Soliman Khatib4Eli Reuveni5Department of Oxidative Stress and Human Diseases, MIGAL – Galilee Research InstituteFaculty of Medicine in the Galilee, Bar-Ilan UniversityFaculty of Medicine in the Galilee, Bar-Ilan UniversityDepartment of Oxidative Stress and Human Diseases, MIGAL – Galilee Research InstituteDepartment of Oxidative Stress and Human Diseases, MIGAL – Galilee Research InstituteFaculty of Medicine in the Galilee, Bar-Ilan UniversityAbstract Background Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an activator of specific cellular pathways, not much attention has been given to its mechanism of action at the genome-wide level. The present study aims to characterize quercetin’s interaction with cellular DNA and to show its subsequent effect on downstream transcription. Results Two massive parallel DNA-sequencing technologies were used: Chem-seq and RNA-seq. We demonstrate that upon binding to DNA or genome-associated proteins, quercetin acts as a cis-regulatory transcription factor for the expression of genes that are involved in the cell cycle, differentiation and development. Conclusions Such findings could provide new and important insights into the mechanisms by which the dietary polyphenol quercetin influences cellular functions.http://link.springer.com/article/10.1186/s12864-019-5966-9PolyphenolQuercetinChem-seqRNA-seqAnticancer |
| spellingShingle | Dana Atrahimovich Avraham O. Samson Yifthah Barsheshet Jacob Vaya Soliman Khatib Eli Reuveni Genome-wide localization of the polyphenol quercetin in human monocytes BMC Genomics Polyphenol Quercetin Chem-seq RNA-seq Anticancer |
| title | Genome-wide localization of the polyphenol quercetin in human monocytes |
| title_full | Genome-wide localization of the polyphenol quercetin in human monocytes |
| title_fullStr | Genome-wide localization of the polyphenol quercetin in human monocytes |
| title_full_unstemmed | Genome-wide localization of the polyphenol quercetin in human monocytes |
| title_short | Genome-wide localization of the polyphenol quercetin in human monocytes |
| title_sort | genome wide localization of the polyphenol quercetin in human monocytes |
| topic | Polyphenol Quercetin Chem-seq RNA-seq Anticancer |
| url | http://link.springer.com/article/10.1186/s12864-019-5966-9 |
| work_keys_str_mv | AT danaatrahimovich genomewidelocalizationofthepolyphenolquercetininhumanmonocytes AT avrahamosamson genomewidelocalizationofthepolyphenolquercetininhumanmonocytes AT yifthahbarsheshet genomewidelocalizationofthepolyphenolquercetininhumanmonocytes AT jacobvaya genomewidelocalizationofthepolyphenolquercetininhumanmonocytes AT solimankhatib genomewidelocalizationofthepolyphenolquercetininhumanmonocytes AT elireuveni genomewidelocalizationofthepolyphenolquercetininhumanmonocytes |