VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran
Objective: The purpose of this study was to determine the prevalence of vancomycin-resistant Staphylococcus aureus isolated from clinical samples in Shiraz hospitals. Methods: From March to December 2012, 100 S. aureus isolates (mainly from wound and blood) were collected from three hospitals in...
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| Format: | Article |
| Language: | English |
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Oman Medical Specialty Board
2014-09-01
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| Series: | Oman Medical Journal |
| Subjects: | |
| Online Access: | http://www.omjournal.org/fultext_PDF.aspx?DetailsID=562&type=fultext |
| _version_ | 1818262011700576256 |
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| author | Sareh Saadat Kavous Solhjoo Mohammad-Javad Norooz-Nejad Akbar Kazemi |
| author_facet | Sareh Saadat Kavous Solhjoo Mohammad-Javad Norooz-Nejad Akbar Kazemi |
| author_sort | Sareh Saadat |
| collection | DOAJ |
| description | Objective: The purpose of this study was to determine the prevalence of vancomycin-resistant Staphylococcus aureus isolated from clinical samples in Shiraz hospitals.
Methods: From March to December 2012, 100 S. aureus isolates (mainly from wound and blood) were collected from three hospitals in Shiraz, south of Iran. After identification of Staphylococcus aureus by biochemical, microbiological and molecular methods, antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion test for 13 different antibiotics. Vancomycin-resistant Staphylococcus aureus isolates were determined by vancomycin agar screening test and PCR for vancomycin resistant genes (vanA and vanB).
Results: The lowest and highest resistance was seen for quinupristin-dalfopristin (n=1) and ampicillin (n=95), respectively. Vancomycin agar screening test showed that 37 isolates can grow on these media. Further study by PCR also detected vanA and/or vanB genes in all of these strains. Also, 19 isolates showed either vanA or vanB but were susceptible according to vancomycin agar screening test. In total, vanA and vanB resistant genes were detected in 34% and 37% of clinical isolates, respectively.
Conclusion: The results showed that the frequency of vancomycin resistance genes (vanA, vanB) is very high in Staphylococcus aureus strains isolated from patients in south of Iran. Thus, urgent interventions are needed to keep the emergence and transmission of these isolates to a minimum. |
| first_indexed | 2024-12-12T18:56:21Z |
| format | Article |
| id | doaj.art-17bbd178a4974de8b247f1706f6e347b |
| institution | Directory Open Access Journal |
| issn | 1999-768X 2070-5204 |
| language | English |
| last_indexed | 2024-12-12T18:56:21Z |
| publishDate | 2014-09-01 |
| publisher | Oman Medical Specialty Board |
| record_format | Article |
| series | Oman Medical Journal |
| spelling | doaj.art-17bbd178a4974de8b247f1706f6e347b2022-12-22T00:15:13ZengOman Medical Specialty BoardOman Medical Journal1999-768X2070-52042014-09-0129533533910.5001/omj.2014.90VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of IranSareh Saadat0Kavous Solhjoo1Mohammad-Javad Norooz-Nejad2Akbar Kazemi3Instructor Microbiology Department, Islamic Azad University, Jahrom Branch (the member of young scientific research club of Islamic Azad university of Jahrom), Jahrom, Iran.Assistant Professor Medical Microbiology Department, School of Medicine, Jahrom University of Medical Sciences, Motahri Street, Jahrom, Iran. Instructor Microbiology Department, Islamic Azad University, Jahrom Branch, Jahrom, Iran.Instructor Medical Microbiology Department, Jahrom University of Medical Sciences, Jahrom, Iran.Objective: The purpose of this study was to determine the prevalence of vancomycin-resistant Staphylococcus aureus isolated from clinical samples in Shiraz hospitals. Methods: From March to December 2012, 100 S. aureus isolates (mainly from wound and blood) were collected from three hospitals in Shiraz, south of Iran. After identification of Staphylococcus aureus by biochemical, microbiological and molecular methods, antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion test for 13 different antibiotics. Vancomycin-resistant Staphylococcus aureus isolates were determined by vancomycin agar screening test and PCR for vancomycin resistant genes (vanA and vanB). Results: The lowest and highest resistance was seen for quinupristin-dalfopristin (n=1) and ampicillin (n=95), respectively. Vancomycin agar screening test showed that 37 isolates can grow on these media. Further study by PCR also detected vanA and/or vanB genes in all of these strains. Also, 19 isolates showed either vanA or vanB but were susceptible according to vancomycin agar screening test. In total, vanA and vanB resistant genes were detected in 34% and 37% of clinical isolates, respectively. Conclusion: The results showed that the frequency of vancomycin resistance genes (vanA, vanB) is very high in Staphylococcus aureus strains isolated from patients in south of Iran. Thus, urgent interventions are needed to keep the emergence and transmission of these isolates to a minimum.http://www.omjournal.org/fultext_PDF.aspx?DetailsID=562&type=fultextStaphylococcus aureusVancomycin ResistancevanAvanBIran |
| spellingShingle | Sareh Saadat Kavous Solhjoo Mohammad-Javad Norooz-Nejad Akbar Kazemi VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran Oman Medical Journal Staphylococcus aureus Vancomycin Resistance vanA vanB Iran |
| title | VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran |
| title_full | VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran |
| title_fullStr | VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran |
| title_full_unstemmed | VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran |
| title_short | VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran |
| title_sort | vana and vanb positive vancomycin resistant staphylococcus aureus among clinical isolates in shiraz south of iran |
| topic | Staphylococcus aureus Vancomycin Resistance vanA vanB Iran |
| url | http://www.omjournal.org/fultext_PDF.aspx?DetailsID=562&type=fultext |
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