Probing the canonicity of the Wnt/Wingless signaling pathway.
The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-04-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5393890?pdf=render |
| _version_ | 1819112445348675584 |
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| author | Alexandra Franz Daria Shlyueva Erich Brunner Alexander Stark Konrad Basler |
| author_facet | Alexandra Franz Daria Shlyueva Erich Brunner Alexander Stark Konrad Basler |
| author_sort | Alexandra Franz |
| collection | DOAJ |
| description | The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear. Here we study on a genome-wide scale the requirement for Armadillo (Arm, Drosophila beta-catenin) and Pangolin (Pan, Drosophila TCF) in the Wnt/Wingless(Wg)-induced transcriptional response of Drosophila Kc cells. Using somatic genetics, we demonstrate that both Arm and Pan are absolutely required for mediating activation and repression of target genes. Furthermore, by means of STARR-sequencing we identified Wnt/Wg-responsive enhancer elements and found that all responsive enhancers depend on Pan. Together, our results confirm the dogma of canonical Wnt/Wg signaling and argue against the existence of distal pathway branches in this system. |
| first_indexed | 2024-12-22T04:13:37Z |
| format | Article |
| id | doaj.art-17bd800fcc1b4a02ba0ae94b2f80778a |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-22T04:13:37Z |
| publishDate | 2017-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-17bd800fcc1b4a02ba0ae94b2f80778a2022-12-21T18:39:28ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-04-01134e100670010.1371/journal.pgen.1006700Probing the canonicity of the Wnt/Wingless signaling pathway.Alexandra FranzDaria ShlyuevaErich BrunnerAlexander StarkKonrad BaslerThe hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear. Here we study on a genome-wide scale the requirement for Armadillo (Arm, Drosophila beta-catenin) and Pangolin (Pan, Drosophila TCF) in the Wnt/Wingless(Wg)-induced transcriptional response of Drosophila Kc cells. Using somatic genetics, we demonstrate that both Arm and Pan are absolutely required for mediating activation and repression of target genes. Furthermore, by means of STARR-sequencing we identified Wnt/Wg-responsive enhancer elements and found that all responsive enhancers depend on Pan. Together, our results confirm the dogma of canonical Wnt/Wg signaling and argue against the existence of distal pathway branches in this system.http://europepmc.org/articles/PMC5393890?pdf=render |
| spellingShingle | Alexandra Franz Daria Shlyueva Erich Brunner Alexander Stark Konrad Basler Probing the canonicity of the Wnt/Wingless signaling pathway. PLoS Genetics |
| title | Probing the canonicity of the Wnt/Wingless signaling pathway. |
| title_full | Probing the canonicity of the Wnt/Wingless signaling pathway. |
| title_fullStr | Probing the canonicity of the Wnt/Wingless signaling pathway. |
| title_full_unstemmed | Probing the canonicity of the Wnt/Wingless signaling pathway. |
| title_short | Probing the canonicity of the Wnt/Wingless signaling pathway. |
| title_sort | probing the canonicity of the wnt wingless signaling pathway |
| url | http://europepmc.org/articles/PMC5393890?pdf=render |
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