Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract
Natural products are considered as an important source for the discovery of new drugs to treat aging-related degenerative diseases and liver injury. The present study profiled the chemical constituents of a methanol extract from Senna singueana bark using HPLC-PDA-ESI-MS/MS and 36 secondary metaboli...
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| Format: | Article |
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MDPI AG
2017-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/9/1502 |
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| author | Mansour Sobeh Mona F. Mahmoud Rehab A. Hasan Haroan Cheng Assem M. El-Shazly Michael Wink |
| author_facet | Mansour Sobeh Mona F. Mahmoud Rehab A. Hasan Haroan Cheng Assem M. El-Shazly Michael Wink |
| author_sort | Mansour Sobeh |
| collection | DOAJ |
| description | Natural products are considered as an important source for the discovery of new drugs to treat aging-related degenerative diseases and liver injury. The present study profiled the chemical constituents of a methanol extract from Senna singueana bark using HPLC-PDA-ESI-MS/MS and 36 secondary metabolites were identified. Proanthocyanidins dominated the extract. Monomers, dimers, trimers of (epi)catechin, (epi)gallocatechin, (epi)guibourtinidol, (ent)cassiaflavan, and (epi)afzelechin represented the major constituents. The extract demonstrated notable antioxidant activities in vitro: In DPPH (EC50 of 20.8 µg/mL), FRAP (18.16 mM FeSO4/mg extract) assays, and total phenolic content amounted 474 mg gallic acid equivalent (GAE)/g extract determined with the Folin-Ciocalteu method. Also, in an in vivo model, the extract increased the survival rate of Caenorhabditis elegans worms pretreated with the pro-oxidant juglone from 43 to 64%, decreased intracellular ROS inside the wild-type nematodes by 47.90%, and induced nuclear translocation of the transcription factor DAF-16 in the transgenic strain TJ356. Additionally, the extract showed a remarkable hepatoprotective activity against d-galactosamine (d-GalN) induced hepatic injury in rats. It significantly reduced elevated AST (aspartate aminotransferase), and total bilirubin. Moreover, the extract induced a strong cytoplasmic Bcl-2 expression indicating suppression of apoptosis. In conclusion, the bark extract of S. sengueana represents an interesting candidate for further research in antioxidants and liver protection. |
| first_indexed | 2024-04-13T19:58:00Z |
| format | Article |
| id | doaj.art-17bf666b1bc94fad99c0b2f6ef659b9c |
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| language | English |
| last_indexed | 2024-04-13T19:58:00Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-17bf666b1bc94fad99c0b2f6ef659b9c2022-12-22T02:32:17ZengMDPI AGMolecules1420-30492017-09-01229150210.3390/molecules22091502molecules22091502Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark ExtractMansour Sobeh0Mona F. Mahmoud1Rehab A. Hasan2Haroan Cheng3Assem M. El-Shazly4Michael Wink5Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, GermanyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Histology, Faculty of Medicine for Girls, Al-Azhar University, Cairo 11651, EgyptInstitute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, GermanyDepartment of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptInstitute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, GermanyNatural products are considered as an important source for the discovery of new drugs to treat aging-related degenerative diseases and liver injury. The present study profiled the chemical constituents of a methanol extract from Senna singueana bark using HPLC-PDA-ESI-MS/MS and 36 secondary metabolites were identified. Proanthocyanidins dominated the extract. Monomers, dimers, trimers of (epi)catechin, (epi)gallocatechin, (epi)guibourtinidol, (ent)cassiaflavan, and (epi)afzelechin represented the major constituents. The extract demonstrated notable antioxidant activities in vitro: In DPPH (EC50 of 20.8 µg/mL), FRAP (18.16 mM FeSO4/mg extract) assays, and total phenolic content amounted 474 mg gallic acid equivalent (GAE)/g extract determined with the Folin-Ciocalteu method. Also, in an in vivo model, the extract increased the survival rate of Caenorhabditis elegans worms pretreated with the pro-oxidant juglone from 43 to 64%, decreased intracellular ROS inside the wild-type nematodes by 47.90%, and induced nuclear translocation of the transcription factor DAF-16 in the transgenic strain TJ356. Additionally, the extract showed a remarkable hepatoprotective activity against d-galactosamine (d-GalN) induced hepatic injury in rats. It significantly reduced elevated AST (aspartate aminotransferase), and total bilirubin. Moreover, the extract induced a strong cytoplasmic Bcl-2 expression indicating suppression of apoptosis. In conclusion, the bark extract of S. sengueana represents an interesting candidate for further research in antioxidants and liver protection.https://www.mdpi.com/1420-3049/22/9/1502Senna singueanaproanthocyanidinsHPLC-PDA-ESI-MS/MSantioxidanthepatoprotectionCaenorhabditis elegans |
| spellingShingle | Mansour Sobeh Mona F. Mahmoud Rehab A. Hasan Haroan Cheng Assem M. El-Shazly Michael Wink Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract Molecules Senna singueana proanthocyanidins HPLC-PDA-ESI-MS/MS antioxidant hepatoprotection Caenorhabditis elegans |
| title | Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract |
| title_full | Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract |
| title_fullStr | Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract |
| title_full_unstemmed | Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract |
| title_short | Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract |
| title_sort | senna singueana antioxidant hepatoprotective antiapoptotic properties and phytochemical profiling of a methanol bark extract |
| topic | Senna singueana proanthocyanidins HPLC-PDA-ESI-MS/MS antioxidant hepatoprotection Caenorhabditis elegans |
| url | https://www.mdpi.com/1420-3049/22/9/1502 |
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