Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection
Gibberella ear rot (GER) caused by <i>Fusarium graminearum</i> (teleomorph <i>Gibberella zeae</i>) is one of the most destructive diseases in maize, which severely reduces yield and contaminates several potential mycotoxins in the grain. However, few efforts had been devoted...
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MDPI AG
2023-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/12/1157 |
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| author | Jihai Zhang Haoya Shi Yong Yang Cheng Zeng Zheyi Jia Tieli Ma Mengyang Wu Juan Du Ning Huang Guangtang Pan Zhilong Li Guangsheng Yuan |
| author_facet | Jihai Zhang Haoya Shi Yong Yang Cheng Zeng Zheyi Jia Tieli Ma Mengyang Wu Juan Du Ning Huang Guangtang Pan Zhilong Li Guangsheng Yuan |
| author_sort | Jihai Zhang |
| collection | DOAJ |
| description | Gibberella ear rot (GER) caused by <i>Fusarium graminearum</i> (teleomorph <i>Gibberella zeae</i>) is one of the most destructive diseases in maize, which severely reduces yield and contaminates several potential mycotoxins in the grain. However, few efforts had been devoted to dissecting the genetic basis of maize GER resistance. In the present study, a genome-wide association study (GWAS) was conducted in a maize association panel consisting of 303 diverse inbred lines. The phenotypes of GER severity were evaluated using kernel bioassay across multiple time points in the laboratory. Then, three models, including the fixed and random model circulating probability unification model (FarmCPU), general linear model (GLM), and mixed linear model (MLM), were conducted simultaneously in GWAS to identify single-nucleotide polymorphisms (SNPs) significantly associated with GER resistance. A total of four individual significant association SNPs with the phenotypic variation explained (PVE) ranging from 3.51 to 6.42% were obtained. Interestingly, the peak SNP (PUT-163a-71443302-3341) with the greatest PVE value, was co-localized in all models. Subsequently, 12 putative genes were captured from the peak SNP, and several of these genes were directly or indirectly involved in disease resistance. Overall, these findings contribute to understanding the complex plant–pathogen interactions in maize GER resistance. The regions and genes identified herein provide a list of candidate targets for further investigation, in addition to the kernel bioassay that can be used for evaluating and selecting elite germplasm resources with GER resistance in maize. |
| first_indexed | 2024-03-08T20:36:30Z |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-08T20:36:30Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-17c1a8562fab4767b5c0a11994e9e7082023-12-22T14:19:20ZengMDPI AGJournal of Fungi2309-608X2023-12-01912115710.3390/jof9121157Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> InfectionJihai Zhang0Haoya Shi1Yong Yang2Cheng Zeng3Zheyi Jia4Tieli Ma5Mengyang Wu6Juan Du7Ning Huang8Guangtang Pan9Zhilong Li10Guangsheng Yuan11Yibin Academy of Agricultural Sciences, Yibin 644600, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaYibin Academy of Agricultural Sciences, Yibin 644600, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaYibin Academy of Agricultural Sciences, Yibin 644600, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaGibberella ear rot (GER) caused by <i>Fusarium graminearum</i> (teleomorph <i>Gibberella zeae</i>) is one of the most destructive diseases in maize, which severely reduces yield and contaminates several potential mycotoxins in the grain. However, few efforts had been devoted to dissecting the genetic basis of maize GER resistance. In the present study, a genome-wide association study (GWAS) was conducted in a maize association panel consisting of 303 diverse inbred lines. The phenotypes of GER severity were evaluated using kernel bioassay across multiple time points in the laboratory. Then, three models, including the fixed and random model circulating probability unification model (FarmCPU), general linear model (GLM), and mixed linear model (MLM), were conducted simultaneously in GWAS to identify single-nucleotide polymorphisms (SNPs) significantly associated with GER resistance. A total of four individual significant association SNPs with the phenotypic variation explained (PVE) ranging from 3.51 to 6.42% were obtained. Interestingly, the peak SNP (PUT-163a-71443302-3341) with the greatest PVE value, was co-localized in all models. Subsequently, 12 putative genes were captured from the peak SNP, and several of these genes were directly or indirectly involved in disease resistance. Overall, these findings contribute to understanding the complex plant–pathogen interactions in maize GER resistance. The regions and genes identified herein provide a list of candidate targets for further investigation, in addition to the kernel bioassay that can be used for evaluating and selecting elite germplasm resources with GER resistance in maize.https://www.mdpi.com/2309-608X/9/12/1157maizeGibberella ear rot<i>Fusarium graminearum</i>genome-wide association studykernel bioassay |
| spellingShingle | Jihai Zhang Haoya Shi Yong Yang Cheng Zeng Zheyi Jia Tieli Ma Mengyang Wu Juan Du Ning Huang Guangtang Pan Zhilong Li Guangsheng Yuan Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection Journal of Fungi maize Gibberella ear rot <i>Fusarium graminearum</i> genome-wide association study kernel bioassay |
| title | Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection |
| title_full | Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection |
| title_fullStr | Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection |
| title_full_unstemmed | Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection |
| title_short | Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to <i>Fusarium graminearum</i> Infection |
| title_sort | kernel bioassay evaluation of maize ear rot and genome wide association analysis for identifying genetic loci associated with resistance to i fusarium graminearum i infection |
| topic | maize Gibberella ear rot <i>Fusarium graminearum</i> genome-wide association study kernel bioassay |
| url | https://www.mdpi.com/2309-608X/9/12/1157 |
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