Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor
Barrier tissue protects the body against external factors by restricting the passage of molecules. The gastrointestinal epithelium is an example of barrier tissue with the primary purpose of allowing the passage of ions and nutrients, while restricting the passage of pathogens and toxins. It is well...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-01-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6374/3/1/44 |
| _version_ | 1818034788198514688 |
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| author | Scherrine Tria Leslie H. Jimison Adel Hama Manuelle Bongo Róisín M. Owens |
| author_facet | Scherrine Tria Leslie H. Jimison Adel Hama Manuelle Bongo Róisín M. Owens |
| author_sort | Scherrine Tria |
| collection | DOAJ |
| description | Barrier tissue protects the body against external factors by restricting the passage of molecules. The gastrointestinal epithelium is an example of barrier tissue with the primary purpose of allowing the passage of ions and nutrients, while restricting the passage of pathogens and toxins. It is well known that the loss of barrier function can be instigated by a decrease in extracellular calcium levels, leading to changes in protein conformation and an increase in paracellular transport. In this study, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid (EGTA), a calcium chelator, was used to disrupt the gastrointestinal epithelial barrier. The effect of EGTA on barrier tissue was monitored by a novel label-free method based on an organic electrochemical transistor (OECT) integrated with living cells and validated against conventional methods for measuring barrier tissue integrity. We demonstrate that the OECT can detect breaches in barrier tissue upon exposure to EGTA with the same sensitivity as existing methods but with increased temporal resolution. Due to the potential of low cost processing techniques and the flexibility in design associated with organic electronics, the OECT has great potential for high-throughput, disposable sensing and diagnostics. |
| first_indexed | 2024-12-10T06:44:43Z |
| format | Article |
| id | doaj.art-17c75eb0d16d4536af31b435268d896e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-10T06:44:43Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-17c75eb0d16d4536af31b435268d896e2022-12-22T01:58:42ZengMDPI AGBiosensors2079-63742013-01-0131445710.3390/bios3010044Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic TransistorScherrine TriaLeslie H. JimisonAdel HamaManuelle BongoRóisín M. OwensBarrier tissue protects the body against external factors by restricting the passage of molecules. The gastrointestinal epithelium is an example of barrier tissue with the primary purpose of allowing the passage of ions and nutrients, while restricting the passage of pathogens and toxins. It is well known that the loss of barrier function can be instigated by a decrease in extracellular calcium levels, leading to changes in protein conformation and an increase in paracellular transport. In this study, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid (EGTA), a calcium chelator, was used to disrupt the gastrointestinal epithelial barrier. The effect of EGTA on barrier tissue was monitored by a novel label-free method based on an organic electrochemical transistor (OECT) integrated with living cells and validated against conventional methods for measuring barrier tissue integrity. We demonstrate that the OECT can detect breaches in barrier tissue upon exposure to EGTA with the same sensitivity as existing methods but with increased temporal resolution. Due to the potential of low cost processing techniques and the flexibility in design associated with organic electronics, the OECT has great potential for high-throughput, disposable sensing and diagnostics.http://www.mdpi.com/2079-6374/3/1/44organic bioelectronicstight junctionsparacellular transportEGTAbarrier tissuetoxicologybiosensingorganic electrochemical transistor |
| spellingShingle | Scherrine Tria Leslie H. Jimison Adel Hama Manuelle Bongo Róisín M. Owens Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor Biosensors organic bioelectronics tight junctions paracellular transport EGTA barrier tissue toxicology biosensing organic electrochemical transistor |
| title | Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor |
| title_full | Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor |
| title_fullStr | Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor |
| title_full_unstemmed | Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor |
| title_short | Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor |
| title_sort | sensing of egta mediated barrier tissue disruption with an organic transistor |
| topic | organic bioelectronics tight junctions paracellular transport EGTA barrier tissue toxicology biosensing organic electrochemical transistor |
| url | http://www.mdpi.com/2079-6374/3/1/44 |
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