A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells
<i>Trypanosoma cruzi</i> causes the lethal Chagas disease, which is endemic in Latin America. Flowers of <i>Moringa oleifera</i> (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to <i>T. cruzi</i> trypomastigotes was previously reported. Here, we s...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/9/8/515 |
| _version_ | 1827709811162087424 |
|---|---|
| author | Isabella Coimbra Vila Nova Leyllane Rafael Moreira Diego José Lira Torres Kamila Kássia dos Santos Oliveira Leydianne Leite de Siqueira Patriota Luana Cassandra Breitenbach Barroso Coelho Patrícia Maria Guedes Paiva Thiago Henrique Napoleão Virgínia Maria Barros de Lorena Emmanuel Viana Pontual |
| author_facet | Isabella Coimbra Vila Nova Leyllane Rafael Moreira Diego José Lira Torres Kamila Kássia dos Santos Oliveira Leydianne Leite de Siqueira Patriota Luana Cassandra Breitenbach Barroso Coelho Patrícia Maria Guedes Paiva Thiago Henrique Napoleão Virgínia Maria Barros de Lorena Emmanuel Viana Pontual |
| author_sort | Isabella Coimbra Vila Nova |
| collection | DOAJ |
| description | <i>Trypanosoma cruzi</i> causes the lethal Chagas disease, which is endemic in Latin America. Flowers of <i>Moringa oleifera</i> (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to <i>T. cruzi</i> trypomastigotes was previously reported. Here, we studied the effects of MoFTI on the viability of human peripheral blood mononuclear cells (PBMCs) as well as on the production of cytokines and nitric oxide (NO) by <i>T. cruzi</i>-infected PBMCs. Incubation with MoFTI (trypsin inhibitory activity: 62 U/mg) led to lysis of trypomastigotes (LC<sub>50</sub> of 43.5 µg/mL) but did not affect the viability of PBMCs when tested at concentrations up to 500 µg/mL. A selectivity index > 11.48 was determined. When <i>T. cruzi</i>-infected PBMCs were treated with MoFTI (43.5 or 87.0 µg/mL), the release of the pro-inflammatory cytokine TNF-α and INF-γ, as well as of NO, was stimulated. The release of the anti-inflammatory cytokine IL-10 also increased. In conclusion, the toxicity to <i>T. cruzi</i> and the production of IL-10 by infected PBMCs treated with MoFTI suggest that this molecule may be able to control parasitemia while regulating the inflammation, preventing the progress of Chagas disease. The data reported here stimulate future investigations concerning the in vivo effects of MoFTI on immune response in Chagas disease. |
| first_indexed | 2024-03-10T17:28:04Z |
| format | Article |
| id | doaj.art-17cd0bf88e8c4afc92829f6b5ce3fc43 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T17:28:04Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-17cd0bf88e8c4afc92829f6b5ce3fc432023-11-20T10:06:37ZengMDPI AGAntibiotics2079-63822020-08-019851510.3390/antibiotics9080515A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human CellsIsabella Coimbra Vila Nova0Leyllane Rafael Moreira1Diego José Lira Torres2Kamila Kássia dos Santos Oliveira3Leydianne Leite de Siqueira Patriota4Luana Cassandra Breitenbach Barroso Coelho5Patrícia Maria Guedes Paiva6Thiago Henrique Napoleão7Virgínia Maria Barros de Lorena8Emmanuel Viana Pontual9Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, BrazilDepartamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, BrazilDepartamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50670-901, BrazilDepartamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife 52171-900, Brazil<i>Trypanosoma cruzi</i> causes the lethal Chagas disease, which is endemic in Latin America. Flowers of <i>Moringa oleifera</i> (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to <i>T. cruzi</i> trypomastigotes was previously reported. Here, we studied the effects of MoFTI on the viability of human peripheral blood mononuclear cells (PBMCs) as well as on the production of cytokines and nitric oxide (NO) by <i>T. cruzi</i>-infected PBMCs. Incubation with MoFTI (trypsin inhibitory activity: 62 U/mg) led to lysis of trypomastigotes (LC<sub>50</sub> of 43.5 µg/mL) but did not affect the viability of PBMCs when tested at concentrations up to 500 µg/mL. A selectivity index > 11.48 was determined. When <i>T. cruzi</i>-infected PBMCs were treated with MoFTI (43.5 or 87.0 µg/mL), the release of the pro-inflammatory cytokine TNF-α and INF-γ, as well as of NO, was stimulated. The release of the anti-inflammatory cytokine IL-10 also increased. In conclusion, the toxicity to <i>T. cruzi</i> and the production of IL-10 by infected PBMCs treated with MoFTI suggest that this molecule may be able to control parasitemia while regulating the inflammation, preventing the progress of Chagas disease. The data reported here stimulate future investigations concerning the in vivo effects of MoFTI on immune response in Chagas disease.https://www.mdpi.com/2079-6382/9/8/515cytokinescytotoxicityimmunomodulatory agent<i>Moringa oleifera</i>protease inhibitortrypanocidal agent |
| spellingShingle | Isabella Coimbra Vila Nova Leyllane Rafael Moreira Diego José Lira Torres Kamila Kássia dos Santos Oliveira Leydianne Leite de Siqueira Patriota Luana Cassandra Breitenbach Barroso Coelho Patrícia Maria Guedes Paiva Thiago Henrique Napoleão Virgínia Maria Barros de Lorena Emmanuel Viana Pontual A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells Antibiotics cytokines cytotoxicity immunomodulatory agent <i>Moringa oleifera</i> protease inhibitor trypanocidal agent |
| title | A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells |
| title_full | A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells |
| title_fullStr | A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells |
| title_full_unstemmed | A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells |
| title_short | A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells |
| title_sort | trypsin inhibitor from i moringa oleifera i flowers modulates the immune response in vitro of i trypanosoma cruzi i infected human cells |
| topic | cytokines cytotoxicity immunomodulatory agent <i>Moringa oleifera</i> protease inhibitor trypanocidal agent |
| url | https://www.mdpi.com/2079-6382/9/8/515 |
| work_keys_str_mv | AT isabellacoimbravilanova atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT leyllanerafaelmoreira atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT diegojoseliratorres atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT kamilakassiadossantosoliveira atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT leydianneleitedesiqueirapatriota atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT luanacassandrabreitenbachbarrosocoelho atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT patriciamariaguedespaiva atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT thiagohenriquenapoleao atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT virginiamariabarrosdelorena atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT emmanuelvianapontual atrypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT isabellacoimbravilanova trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT leyllanerafaelmoreira trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT diegojoseliratorres trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT kamilakassiadossantosoliveira trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT leydianneleitedesiqueirapatriota trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT luanacassandrabreitenbachbarrosocoelho trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT patriciamariaguedespaiva trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT thiagohenriquenapoleao trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT virginiamariabarrosdelorena trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells AT emmanuelvianapontual trypsininhibitorfromimoringaoleiferaiflowersmodulatestheimmuneresponseinvitroofitrypanosomacruziiinfectedhumancells |