Plant germplasm and extreme conditions of outer space
The extreme temperature fluctuations and the vacuum of the space environment make growing plants in outer space challenging. To simulate the temperature fluctuations and vacuum conditions associated with space environments, dry tomato seeds were placed in a thermal cycle simulator and vacuum simulat...
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| Format: | Article |
| Language: | fas |
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Aerospace Research Institute
2023-10-01
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| Series: | فصلنامه علوم و فناوری فضایی |
| Subjects: | |
| Online Access: | https://jsst.ias.ir/article_181547_7199bac6de86456dc0b96cd778130717.pdf |
| _version_ | 1797353926686670848 |
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| author | Fateme Mousavi |
| author_facet | Fateme Mousavi |
| author_sort | Fateme Mousavi |
| collection | DOAJ |
| description | The extreme temperature fluctuations and the vacuum of the space environment make growing plants in outer space challenging. To simulate the temperature fluctuations and vacuum conditions associated with space environments, dry tomato seeds were placed in a thermal cycle simulator and vacuum simulator chamber of space systems, respectively. A Bradford method was used to determine the total protein content of each group of seeds. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis was used to separate proteins. The seed of the thermal cycle group had the highest protein content (26 to 31 mg/ml), followed by control seeds (8-10 mg/ml) and the vacuum seeds (4-5.6 mg/ml). The molecular weights of the peptides ranged from 8 to 42 kDa. The intensity of the protein bands was significantly different in the thermal cycle group from the other two groups, and vacuum group had the lowest intensity. Water and oil released from seeds in the vacuum environment resulted in a reduction of protein content. In the thermal cycle group, the total protein content and the intensity of the bands were significantly higher than those treated with the control group, which can be attributed to the degradation of storage proteins involved in seed germination in the control group. |
| first_indexed | 2024-03-08T13:37:00Z |
| format | Article |
| id | doaj.art-17cea2d7f8e84ddb9cadab202268ed2f |
| institution | Directory Open Access Journal |
| issn | 2008-4560 2423-4516 |
| language | fas |
| last_indexed | 2024-03-08T13:37:00Z |
| publishDate | 2023-10-01 |
| publisher | Aerospace Research Institute |
| record_format | Article |
| series | فصلنامه علوم و فناوری فضایی |
| spelling | doaj.art-17cea2d7f8e84ddb9cadab202268ed2f2024-01-16T14:11:28ZfasAerospace Research Instituteفصلنامه علوم و فناوری فضایی2008-45602423-45162023-10-0116ویژه نامه انگلیسی657110.22034/jsst.2023.1460181547Plant germplasm and extreme conditions of outer spaceFateme Mousavi0Assistant Professor, Aerospace Research Institute of the Ministry of Science, Research and Technology, Tehran, IranThe extreme temperature fluctuations and the vacuum of the space environment make growing plants in outer space challenging. To simulate the temperature fluctuations and vacuum conditions associated with space environments, dry tomato seeds were placed in a thermal cycle simulator and vacuum simulator chamber of space systems, respectively. A Bradford method was used to determine the total protein content of each group of seeds. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis was used to separate proteins. The seed of the thermal cycle group had the highest protein content (26 to 31 mg/ml), followed by control seeds (8-10 mg/ml) and the vacuum seeds (4-5.6 mg/ml). The molecular weights of the peptides ranged from 8 to 42 kDa. The intensity of the protein bands was significantly different in the thermal cycle group from the other two groups, and vacuum group had the lowest intensity. Water and oil released from seeds in the vacuum environment resulted in a reduction of protein content. In the thermal cycle group, the total protein content and the intensity of the bands were significantly higher than those treated with the control group, which can be attributed to the degradation of storage proteins involved in seed germination in the control group.https://jsst.ias.ir/article_181547_7199bac6de86456dc0b96cd778130717.pdfsds-pagesolanum lycopersicumspacespacecraftthermal cyclevacuum |
| spellingShingle | Fateme Mousavi Plant germplasm and extreme conditions of outer space فصلنامه علوم و فناوری فضایی sds-page solanum lycopersicum space spacecraft thermal cycle vacuum |
| title | Plant germplasm and extreme conditions of outer space |
| title_full | Plant germplasm and extreme conditions of outer space |
| title_fullStr | Plant germplasm and extreme conditions of outer space |
| title_full_unstemmed | Plant germplasm and extreme conditions of outer space |
| title_short | Plant germplasm and extreme conditions of outer space |
| title_sort | plant germplasm and extreme conditions of outer space |
| topic | sds-page solanum lycopersicum space spacecraft thermal cycle vacuum |
| url | https://jsst.ias.ir/article_181547_7199bac6de86456dc0b96cd778130717.pdf |
| work_keys_str_mv | AT fatememousavi plantgermplasmandextremeconditionsofouterspace |