Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture
Introduction: Bioflavonoids delphinidin (2-(3,4,5-Trihydroxyphenyl)chromenylium-3,5,7-triol) and luteolin (2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) have been recognized as promising antioxidants and anticancer substances. Due to their extensive use, the goal of the research was to determi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sarajevo
2015-08-01
|
| Series: | Journal of Health Sciences |
| Subjects: | |
| Online Access: | https://www.jhsci.ba/ojs/index.php/jhsci/article/view/391 |
| _version_ | 1811287097017892864 |
|---|---|
| author | Jasmin Ezić Amina Kugić Maida Hadžić Anja Haverić Kasim Bajrović Sanin Haverić |
| author_facet | Jasmin Ezić Amina Kugić Maida Hadžić Anja Haverić Kasim Bajrović Sanin Haverić |
| author_sort | Jasmin Ezić |
| collection | DOAJ |
| description | Introduction: Bioflavonoids delphinidin (2-(3,4,5-Trihydroxyphenyl)chromenylium-3,5,7-triol) and luteolin (2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) have been recognized as promising antioxidants and anticancer substances. Due to their extensive use, the goal of the research was to determine whether they have any genotoxic potential in vitro.
Methods: Analysis of genotoxic potential was performed applying chromosome aberrations test in human lymphocyte culture, as this kind of research was not conducted abundantly for these two bioflavonoids. Delphinidin and luteolin were dissolved in DMSO and added to cultures in final concentrations of 25, 50 and 100 μM.
Results: In human lymphocytes cultures Delphinidin induced PCDs in all treatments, potentially affecting the cell cycle and topoisomerase II activity. In concentration of 50 μM luteolin showed strong genotoxic effects and caused significant reduction of cell proliferation.
Conclusion: Luteolin exhibited certain genotoxic and cytostatic potential. Delphinidin was not considered genotoxic, however its impact on mitosis, especially topoisomerase II activity, was revealed. |
| first_indexed | 2024-04-13T03:12:46Z |
| format | Article |
| id | doaj.art-17cf26f3b346456193d7cabf30681f98 |
| institution | Directory Open Access Journal |
| issn | 2232-7576 1986-8049 |
| language | English |
| last_indexed | 2024-04-13T03:12:46Z |
| publishDate | 2015-08-01 |
| publisher | University of Sarajevo |
| record_format | Article |
| series | Journal of Health Sciences |
| spelling | doaj.art-17cf26f3b346456193d7cabf30681f982022-12-22T03:05:00ZengUniversity of SarajevoJournal of Health Sciences2232-75761986-80492015-08-015210.17532/jhsci.2015.248165Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte CultureJasmin Ezić0Amina Kugić1Maida Hadžić2Anja Haverić3Kasim Bajrović4Sanin Haverić5Institute for genetic engineering and biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaInstitute for genetic engineering and biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaInstitute for genetic engineering and biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaInstitute for genetic engineering and biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaInstitute for genetic engineering and biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaInstitute for genetic engineering and biotechnology, University of Sarajevo, Sarajevo, Bosnia and HerzegovinaIntroduction: Bioflavonoids delphinidin (2-(3,4,5-Trihydroxyphenyl)chromenylium-3,5,7-triol) and luteolin (2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) have been recognized as promising antioxidants and anticancer substances. Due to their extensive use, the goal of the research was to determine whether they have any genotoxic potential in vitro. Methods: Analysis of genotoxic potential was performed applying chromosome aberrations test in human lymphocyte culture, as this kind of research was not conducted abundantly for these two bioflavonoids. Delphinidin and luteolin were dissolved in DMSO and added to cultures in final concentrations of 25, 50 and 100 μM. Results: In human lymphocytes cultures Delphinidin induced PCDs in all treatments, potentially affecting the cell cycle and topoisomerase II activity. In concentration of 50 μM luteolin showed strong genotoxic effects and caused significant reduction of cell proliferation. Conclusion: Luteolin exhibited certain genotoxic and cytostatic potential. Delphinidin was not considered genotoxic, however its impact on mitosis, especially topoisomerase II activity, was revealed.https://www.jhsci.ba/ojs/index.php/jhsci/article/view/391Chromosome aberrationscell proliferationbioflavonoides. |
| spellingShingle | Jasmin Ezić Amina Kugić Maida Hadžić Anja Haverić Kasim Bajrović Sanin Haverić Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture Journal of Health Sciences Chromosome aberrations cell proliferation bioflavonoides. |
| title | Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture |
| title_full | Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture |
| title_fullStr | Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture |
| title_full_unstemmed | Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture |
| title_short | Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture |
| title_sort | analysis of delphinidin and luteolin genotoxicity in human lymphocyte culture |
| topic | Chromosome aberrations cell proliferation bioflavonoides. |
| url | https://www.jhsci.ba/ojs/index.php/jhsci/article/view/391 |
| work_keys_str_mv | AT jasminezic analysisofdelphinidinandluteolingenotoxicityinhumanlymphocyteculture AT aminakugic analysisofdelphinidinandluteolingenotoxicityinhumanlymphocyteculture AT maidahadzic analysisofdelphinidinandluteolingenotoxicityinhumanlymphocyteculture AT anjahaveric analysisofdelphinidinandluteolingenotoxicityinhumanlymphocyteculture AT kasimbajrovic analysisofdelphinidinandluteolingenotoxicityinhumanlymphocyteculture AT saninhaveric analysisofdelphinidinandluteolingenotoxicityinhumanlymphocyteculture |