Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells
Summary: With the continuous emergence of highly transmissible SARS-CoV-2 variants, the comparison of their infectivity has become a critical issue for public health. However, a direct assessment of the viral characteristic has been challenging because of the lack of appropriate experimental models...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222018430 |
| _version_ | 1811212098482470912 |
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| author | Taewoo Kim Kyoung Il Min Jeong-Sun Yang Jun Won Kim Junhyung Cho Yun Ho Kim Jeong Seok Lee Young Tae Kim Kyung-Chang Kim Jeong Yeon Kim Kwon Joong Na Joo-Yeon Lee Young Seok Ju |
| author_facet | Taewoo Kim Kyoung Il Min Jeong-Sun Yang Jun Won Kim Junhyung Cho Yun Ho Kim Jeong Seok Lee Young Tae Kim Kyung-Chang Kim Jeong Yeon Kim Kwon Joong Na Joo-Yeon Lee Young Seok Ju |
| author_sort | Taewoo Kim |
| collection | DOAJ |
| description | Summary: With the continuous emergence of highly transmissible SARS-CoV-2 variants, the comparison of their infectivity has become a critical issue for public health. However, a direct assessment of the viral characteristic has been challenging because of the lack of appropriate experimental models and efficient methods. Here, we integrated human alveolar organoids and single-cell transcriptome sequencing to facilitate the evaluation. In a proof-of-concept study with four highly transmissible SARS-CoV-2 variants, including GR (B.1.1.119), Alpha (B.1.1.7), Delta (B.1.617.2), and Omicron (BA.1), a rapid evaluation of the relative infectivity was possible. Our system demonstrates that the Omicron variant is 5- to 7-fold more infectious to human alveolar cells than the other SARS-CoV-2 variants at the initial stage of infection. To our knowledge, for the first time, this study measures the relative infectivity of the Omicron variant under multiple virus co-infection and provides new experimental procedures that can be applied to monitor emerging viral variants. |
| first_indexed | 2024-04-12T05:23:07Z |
| format | Article |
| id | doaj.art-17d26891f38b4eb886c2235b690849f4 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T05:23:07Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-17d26891f38b4eb886c2235b690849f42022-12-22T03:46:22ZengElsevieriScience2589-00422022-12-012512105571Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cellsTaewoo Kim0Kyoung Il Min1Jeong-Sun Yang2Jun Won Kim3Junhyung Cho4Yun Ho Kim5Jeong Seok Lee6Young Tae Kim7Kyung-Chang Kim8Jeong Yeon Kim9Kwon Joong Na10Joo-Yeon Lee11Young Seok Ju12Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of KoreaGraduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of KoreaDivision of Emerging Virus & Vector Research, Center for Emerging Virus Research, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of KoreaDivision of Emerging Virus & Vector Research, Center for Emerging Virus Research, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of KoreaDivision of Emerging Virus & Vector Research, Center for Emerging Virus Research, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University Cancer Research Institute, Seoul 03080, Republic of KoreaGraduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea; GENOME INSIGHT Inc., Daejeon 34051, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University Cancer Research Institute, Seoul 03080, Republic of KoreaDivision of Emerging Virus & Vector Research, Center for Emerging Virus Research, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of KoreaGENOME INSIGHT Inc., Daejeon 34051, Republic of KoreaDepartment of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University Cancer Research Institute, Seoul 03080, Republic of Korea; Corresponding authorDivision of Emerging Virus & Vector Research, Center for Emerging Virus Research, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju 28159, Republic of Korea; Corresponding authorGraduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea; GENOME INSIGHT Inc., Daejeon 34051, Republic of Korea; Corresponding authorSummary: With the continuous emergence of highly transmissible SARS-CoV-2 variants, the comparison of their infectivity has become a critical issue for public health. However, a direct assessment of the viral characteristic has been challenging because of the lack of appropriate experimental models and efficient methods. Here, we integrated human alveolar organoids and single-cell transcriptome sequencing to facilitate the evaluation. In a proof-of-concept study with four highly transmissible SARS-CoV-2 variants, including GR (B.1.1.119), Alpha (B.1.1.7), Delta (B.1.617.2), and Omicron (BA.1), a rapid evaluation of the relative infectivity was possible. Our system demonstrates that the Omicron variant is 5- to 7-fold more infectious to human alveolar cells than the other SARS-CoV-2 variants at the initial stage of infection. To our knowledge, for the first time, this study measures the relative infectivity of the Omicron variant under multiple virus co-infection and provides new experimental procedures that can be applied to monitor emerging viral variants.http://www.sciencedirect.com/science/article/pii/S2589004222018430Public healthVirology |
| spellingShingle | Taewoo Kim Kyoung Il Min Jeong-Sun Yang Jun Won Kim Junhyung Cho Yun Ho Kim Jeong Seok Lee Young Tae Kim Kyung-Chang Kim Jeong Yeon Kim Kwon Joong Na Joo-Yeon Lee Young Seok Ju Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells iScience Public health Virology |
| title | Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells |
| title_full | Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells |
| title_fullStr | Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells |
| title_full_unstemmed | Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells |
| title_short | Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells |
| title_sort | relative infectivity of the sars cov 2 omicron variant in human alveolar cells |
| topic | Public health Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222018430 |
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