Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking
Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems su...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722001836 |
Summary: | Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment. Although this bioprinting system uses a limited crosslinking agent, it is a versatile and advanced continuous multi-material printing technique.For complete details on the use and execution of this protocol, please refer to Lee et al. (2021). |
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ISSN: | 2666-1667 |