Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking

Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems su...

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Bibliographic Details
Main Authors: Gihyun Lee, Soo Jee Kim, Je-Kyun Park
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001836
Description
Summary:Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment. Although this bioprinting system uses a limited crosslinking agent, it is a versatile and advanced continuous multi-material printing technique.For complete details on the use and execution of this protocol, please refer to Lee et al. (2021).
ISSN:2666-1667