Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking
Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems su...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722001836 |
| _version_ | 1818264655206809600 |
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| author | Gihyun Lee Soo Jee Kim Je-Kyun Park |
| author_facet | Gihyun Lee Soo Jee Kim Je-Kyun Park |
| author_sort | Gihyun Lee |
| collection | DOAJ |
| description | Summary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment. Although this bioprinting system uses a limited crosslinking agent, it is a versatile and advanced continuous multi-material printing technique.For complete details on the use and execution of this protocol, please refer to Lee et al. (2021). |
| first_indexed | 2024-12-12T19:38:22Z |
| format | Article |
| id | doaj.art-17d6ef67a6464b1694c106805b8764b3 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T19:38:22Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-17d6ef67a6464b1694c106805b8764b32022-12-22T00:14:14ZengElsevierSTAR Protocols2666-16672022-06-0132101303Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinkingGihyun Lee0Soo Jee Kim1Je-Kyun Park2Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Corresponding authorDepartment of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreaDepartment of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; KAIST Institute for Health Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Corresponding authorSummary: Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment. Although this bioprinting system uses a limited crosslinking agent, it is a versatile and advanced continuous multi-material printing technique.For complete details on the use and execution of this protocol, please refer to Lee et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722001836Cell cultureCancerTissue EngineeringBiotechnology and bioengineering |
| spellingShingle | Gihyun Lee Soo Jee Kim Je-Kyun Park Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking STAR Protocols Cell culture Cancer Tissue Engineering Biotechnology and bioengineering |
| title | Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking |
| title_full | Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking |
| title_fullStr | Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking |
| title_full_unstemmed | Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking |
| title_short | Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking |
| title_sort | bioprinting of heterogeneous and multilayered cell hydrogel constructs using continuous multi material printing and aerosol based crosslinking |
| topic | Cell culture Cancer Tissue Engineering Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722001836 |
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