Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR
We identified a species level of the fungal cultures isolated from selected crop seeds using traditional method and BIO-PCR. The use of BIO-PCR did not correspond completely to the morphological analyses. Both methods showed increased infection with F. poae in winter wheat seed sample originated fro...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2012-12-01
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| Series: | Acta Agrobotanica |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/aa/article/view/1908 |
| _version_ | 1818458061893795840 |
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| author | Tomasz Kulik Gabriel Fordoński Agnieszka Pszczółkowska Krystyna Płodzień Jacek Olszewski |
| author_facet | Tomasz Kulik Gabriel Fordoński Agnieszka Pszczółkowska Krystyna Płodzień Jacek Olszewski |
| author_sort | Tomasz Kulik |
| collection | DOAJ |
| description | We identified a species level of the fungal cultures isolated from selected crop seeds using traditional method and BIO-PCR. The use of BIO-PCR did not correspond completely to the morphological analyses. Both methods showed increased infection with F. poae in winter wheat seed sample originated from north Poland. Fungal culture No 40 (isolated from faba bean and identified with traditional method as mixed culture with F. culmorum and F. graminearum) did not produce expected product after PCR reaction with species specific primers OPT18F470, OPT18R470. However, the use of additional primers Fc01F, Fc01R allowed for reliable identification of F. culmorum in the culture. |
| first_indexed | 2024-12-14T22:52:29Z |
| format | Article |
| id | doaj.art-17dab640cf99495ea3c3c00fad686120 |
| institution | Directory Open Access Journal |
| issn | 2300-357X |
| language | English |
| last_indexed | 2024-12-14T22:52:29Z |
| publishDate | 2012-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Agrobotanica |
| spelling | doaj.art-17dab640cf99495ea3c3c00fad6861202022-12-21T22:44:40ZengPolish Botanical SocietyActa Agrobotanica2300-357X2012-12-01582335410.5586/aa.2005.0321520Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCRTomasz Kulik0Gabriel Fordoński1Agnieszka Pszczółkowska2Krystyna Płodzień3Jacek Olszewski4University of Warmia and Mazury in OlsztynUniversity of Warmia and Mazury in OlsztynUniversity of Warmia and Mazury in OlsztynUniversity of Warmia and Mazury in OlsztynUniversity of Warmia and Mazury in OlsztynWe identified a species level of the fungal cultures isolated from selected crop seeds using traditional method and BIO-PCR. The use of BIO-PCR did not correspond completely to the morphological analyses. Both methods showed increased infection with F. poae in winter wheat seed sample originated from north Poland. Fungal culture No 40 (isolated from faba bean and identified with traditional method as mixed culture with F. culmorum and F. graminearum) did not produce expected product after PCR reaction with species specific primers OPT18F470, OPT18R470. However, the use of additional primers Fc01F, Fc01R allowed for reliable identification of F. culmorum in the culture.https://pbsociety.org.pl/journals/index.php/aa/article/view/1908Fusarium spp.identyfikacjaBIO-PCRSCAR |
| spellingShingle | Tomasz Kulik Gabriel Fordoński Agnieszka Pszczółkowska Krystyna Płodzień Jacek Olszewski Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR Acta Agrobotanica Fusarium spp. identyfikacja BIO-PCR SCAR |
| title | Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR |
| title_full | Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR |
| title_fullStr | Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR |
| title_full_unstemmed | Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR |
| title_short | Identification of some Fusarium species from selected crop seeds using traditional method and BIO-PCR |
| title_sort | identification of some fusarium species from selected crop seeds using traditional method and bio pcr |
| topic | Fusarium spp. identyfikacja BIO-PCR SCAR |
| url | https://pbsociety.org.pl/journals/index.php/aa/article/view/1908 |
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