Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator
Microtubules (MTs) are important structures of the cytoskeleton in neurons. Mammalian brain MTs act as biomolecular transistors that generate highly synchronous electrical oscillations. However, their role in brain function is largely unknown. To gain insight into the MT electrical oscillatory activ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2021.727025/full |
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| author | Brenda C. Gutierrez Marcelo R. Pita Almenar Luciano J. Martínez Manuel Siñeriz Louis Virginia H. Albarracín María del Rocío Cantero Horacio F. Cantiello |
| author_facet | Brenda C. Gutierrez Marcelo R. Pita Almenar Luciano J. Martínez Manuel Siñeriz Louis Virginia H. Albarracín María del Rocío Cantero Horacio F. Cantiello |
| author_sort | Brenda C. Gutierrez |
| collection | DOAJ |
| description | Microtubules (MTs) are important structures of the cytoskeleton in neurons. Mammalian brain MTs act as biomolecular transistors that generate highly synchronous electrical oscillations. However, their role in brain function is largely unknown. To gain insight into the MT electrical oscillatory activity of the brain, we turned to the honeybee (Apis mellifera) as a useful model to isolate brains and MTs. The patch clamp technique was applied to MT sheets of purified honeybee brain MTs. High resistance seal patches showed electrical oscillations that linearly depended on the holding potential between ± 200 mV and had an average conductance in the order of ~9 nS. To place these oscillations in the context of the brain, we also explored local field potential (LFP) recordings from the Triton X-permeabilized whole honeybee brain unmasking spontaneous oscillations after but not before tissue permeabilization. Frequency domain spectral analysis of time records indicated at least two major peaks at approximately ~38 Hz and ~93 Hz in both preparations. The present data provide evidence that MT electrical oscillations are a novel signaling mechanism implicated in brain wave activity observed in the insect brain. |
| first_indexed | 2024-12-17T04:56:57Z |
| format | Article |
| id | doaj.art-17dad0d871864490860a6f2f3662ea56 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-17T04:56:57Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-17dad0d871864490860a6f2f3662ea562022-12-21T22:02:41ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992021-09-011410.3389/fnmol.2021.727025727025Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central OscillatorBrenda C. Gutierrez0Marcelo R. Pita Almenar1Luciano J. Martínez2Manuel Siñeriz Louis3Virginia H. Albarracín4María del Rocío Cantero5Horacio F. Cantiello6Laboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero, ArgentinaLaboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero, ArgentinaCentro Integral de Microscopía Electrónica (CIME-CONICET-UNT), Tucumán, ArgentinaCentro Integral de Microscopía Electrónica (CIME-CONICET-UNT), Tucumán, ArgentinaCentro Integral de Microscopía Electrónica (CIME-CONICET-UNT), Tucumán, ArgentinaLaboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero, ArgentinaLaboratorio de Canales Iónicos, Instituto Multidisciplinario de Salud, Tecnología y Desarrollo (IMSaTeD, CONICET-UNSE), Santiago del Estero, ArgentinaMicrotubules (MTs) are important structures of the cytoskeleton in neurons. Mammalian brain MTs act as biomolecular transistors that generate highly synchronous electrical oscillations. However, their role in brain function is largely unknown. To gain insight into the MT electrical oscillatory activity of the brain, we turned to the honeybee (Apis mellifera) as a useful model to isolate brains and MTs. The patch clamp technique was applied to MT sheets of purified honeybee brain MTs. High resistance seal patches showed electrical oscillations that linearly depended on the holding potential between ± 200 mV and had an average conductance in the order of ~9 nS. To place these oscillations in the context of the brain, we also explored local field potential (LFP) recordings from the Triton X-permeabilized whole honeybee brain unmasking spontaneous oscillations after but not before tissue permeabilization. Frequency domain spectral analysis of time records indicated at least two major peaks at approximately ~38 Hz and ~93 Hz in both preparations. The present data provide evidence that MT electrical oscillations are a novel signaling mechanism implicated in brain wave activity observed in the insect brain.https://www.frontiersin.org/articles/10.3389/fnmol.2021.727025/fullmicrotubuletubulinhoneybeeelectrical oscillationsbrainlocal field potentials |
| spellingShingle | Brenda C. Gutierrez Marcelo R. Pita Almenar Luciano J. Martínez Manuel Siñeriz Louis Virginia H. Albarracín María del Rocío Cantero Horacio F. Cantiello Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator Frontiers in Molecular Neuroscience microtubule tubulin honeybee electrical oscillations brain local field potentials |
| title | Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator |
| title_full | Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator |
| title_fullStr | Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator |
| title_full_unstemmed | Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator |
| title_short | Honeybee Brain Oscillations Are Generated by Microtubules. The Concept of a Brain Central Oscillator |
| title_sort | honeybee brain oscillations are generated by microtubules the concept of a brain central oscillator |
| topic | microtubule tubulin honeybee electrical oscillations brain local field potentials |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2021.727025/full |
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