Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces
ABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesio...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-06-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00277-19 |
| _version_ | 1818398499684745216 |
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| author | Claire Valotteau Valeria Prystopiuk Brendan P. Cormack Yves F. Dufrêne |
| author_facet | Claire Valotteau Valeria Prystopiuk Brendan P. Cormack Yves F. Dufrêne |
| author_sort | Claire Valotteau |
| collection | DOAJ |
| description | ABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of them also promote nonspecific adhesion to abiotic surfaces, though this is incompletely understood. Here we address this issue using a combination of genetics and single-cell force measurements. By quantifying the forces driving the attachment of single C. glabrata cells to hydrophobic and hydrophilic substrates, we show that cell adhesion is strongly increased by loss of Sir-mediated silencing. Using a series of mutant strains lacking specific EPA genes, we demonstrate unexpectedly that three major Epa proteins, Epa1, Epa6, and Epa7, primarily contribute to both hydrophilic and hydrophobic interactions, suggesting a broad role for the Epa adhesins in mediating specific and nonspecific adherence and implicating Epa genes in biofilm formation on abiotic surfaces. IMPORTANCE Candida glabrata cell wall proteins mediate the attachment of C. glabrata to abiotic surfaces through molecular interactions that are poorly understood. Here, we study the forces engaged in Epa-dependent adhesion using single-cell techniques. Fungal adhesion to hydrophilic and hydrophobic substrates involves mainly three Epa proteins, suggesting a broad role for the Epa adhesins in mediating adherence. These proteins might represent a potential target for the development of innovative antifungal drugs. |
| first_indexed | 2024-12-14T07:05:46Z |
| format | Article |
| id | doaj.art-17dcd5c404b84f52b17a49c61323ea95 |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-14T07:05:46Z |
| publishDate | 2019-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-17dcd5c404b84f52b17a49c61323ea952022-12-21T23:12:13ZengAmerican Society for MicrobiologymSphere2379-50422019-06-014310.1128/mSphere.00277-19Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic SurfacesClaire Valotteau0Valeria Prystopiuk1Brendan P. Cormack2Yves F. Dufrêne3Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Louvain-la-Neuve, BelgiumLouvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Louvain-la-Neuve, BelgiumDepartment of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USALouvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Louvain-la-Neuve, BelgiumABSTRACT The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of them also promote nonspecific adhesion to abiotic surfaces, though this is incompletely understood. Here we address this issue using a combination of genetics and single-cell force measurements. By quantifying the forces driving the attachment of single C. glabrata cells to hydrophobic and hydrophilic substrates, we show that cell adhesion is strongly increased by loss of Sir-mediated silencing. Using a series of mutant strains lacking specific EPA genes, we demonstrate unexpectedly that three major Epa proteins, Epa1, Epa6, and Epa7, primarily contribute to both hydrophilic and hydrophobic interactions, suggesting a broad role for the Epa adhesins in mediating specific and nonspecific adherence and implicating Epa genes in biofilm formation on abiotic surfaces. IMPORTANCE Candida glabrata cell wall proteins mediate the attachment of C. glabrata to abiotic surfaces through molecular interactions that are poorly understood. Here, we study the forces engaged in Epa-dependent adhesion using single-cell techniques. Fungal adhesion to hydrophilic and hydrophobic substrates involves mainly three Epa proteins, suggesting a broad role for the Epa adhesins in mediating adherence. These proteins might represent a potential target for the development of innovative antifungal drugs.https://journals.asm.org/doi/10.1128/mSphere.00277-19Candida glabrataadhesionAFMEPA |
| spellingShingle | Claire Valotteau Valeria Prystopiuk Brendan P. Cormack Yves F. Dufrêne Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces mSphere Candida glabrata adhesion AFM EPA |
| title | Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces |
| title_full | Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces |
| title_fullStr | Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces |
| title_full_unstemmed | Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces |
| title_short | Atomic Force Microscopy Demonstrates that <named-content content-type="genus-species">Candida glabrata</named-content> Uses Three Epa Proteins To Mediate Adhesion to Abiotic Surfaces |
| title_sort | atomic force microscopy demonstrates that named content content type genus species candida glabrata named content uses three epa proteins to mediate adhesion to abiotic surfaces |
| topic | Candida glabrata adhesion AFM EPA |
| url | https://journals.asm.org/doi/10.1128/mSphere.00277-19 |
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