Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene
Delta bovine papillomaviruses (δBPVs) causes fibropapillomas or bladder cancer in cattle. E5 is the major oncogene of δBPVs; however, the influence that E5 oncogene has on host microRNA (miRNA) and mRNA expression profiles remains little elucidated. In the present study, small RNA sequencing and RNA...
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PeerJ Inc.
2019-11-01
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| Online Access: | https://peerj.com/articles/8098.pdf |
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| author | Feng Pang Zhen Chen Chengqiang Wang Mengmeng Zhang Zhenxing Zhang Xiaohong Yang Yiying Zheng Ang Liu Yiwen Cheng Jie Chen Bin Li Li Du Fengyang Wang |
| author_facet | Feng Pang Zhen Chen Chengqiang Wang Mengmeng Zhang Zhenxing Zhang Xiaohong Yang Yiying Zheng Ang Liu Yiwen Cheng Jie Chen Bin Li Li Du Fengyang Wang |
| author_sort | Feng Pang |
| collection | DOAJ |
| description | Delta bovine papillomaviruses (δBPVs) causes fibropapillomas or bladder cancer in cattle. E5 is the major oncogene of δBPVs; however, the influence that E5 oncogene has on host microRNA (miRNA) and mRNA expression profiles remains little elucidated. In the present study, small RNA sequencing and RNA sequencing were used to explore alterations in miRNAs and mRNAs in E5 over-expressing Madin-Darby bovine kidney (MDBK) cells compared with controls. In total, 77 miRNAs (including 30 bovine-derived miRNAs) and 223 genes were differentially expressed (DE) following E5 overexpression. The dysregulated genes were mainly involved in metabolic and biosynthetic processes. We constructed a potential miRNA-gene regulatory network from the differentially expressed genes (DEGs) and DE miRNAs. Finally, 22 DEGs and nine DE miRNAs were selected for RT-qPCR validation. Of these, downregulation of six miRNAs, bta-miR-34c, bta-miR-122, bta-miR-195, bta-miR-449b, bta-miR-2425-5p, and bta-miR-2428-3p were confirmed; In addition, upregulation of 16 genes, ACSS2, DDIT4, INHBE, INSIG1, PNRC1, PSAT1, PSPH, PYCR1, SC4MOL, SLC34A2, SCD, SPARC, IDI1, PCK2, HMGCS1, and SMIM14 and downregulation of two genes, BATF3 and WFDC2 were confirmed. Specially, bta-miR-34c and bta-miR-449b potentially regulated PYCR1 and DDIT4, which were involved in cancer progression and angiogenesis. Our study presented for the first time the comprehensive miRNA and mRNA alterations in MDBK cells expressing the BPV E5 oncogene, providing new insights into the tumorigenesis induced by BPV E5. |
| first_indexed | 2024-03-09T06:46:57Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:46:57Z |
| publishDate | 2019-11-01 |
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| spelling | doaj.art-17dd9538eb5b484e8f17dd875dd5b2fc2023-12-03T10:34:08ZengPeerJ Inc.PeerJ2167-83592019-11-017e809810.7717/peerj.8098Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogeneFeng PangZhen ChenChengqiang WangMengmeng ZhangZhenxing ZhangXiaohong YangYiying ZhengAng LiuYiwen ChengJie ChenBin LiLi DuFengyang WangDelta bovine papillomaviruses (δBPVs) causes fibropapillomas or bladder cancer in cattle. E5 is the major oncogene of δBPVs; however, the influence that E5 oncogene has on host microRNA (miRNA) and mRNA expression profiles remains little elucidated. In the present study, small RNA sequencing and RNA sequencing were used to explore alterations in miRNAs and mRNAs in E5 over-expressing Madin-Darby bovine kidney (MDBK) cells compared with controls. In total, 77 miRNAs (including 30 bovine-derived miRNAs) and 223 genes were differentially expressed (DE) following E5 overexpression. The dysregulated genes were mainly involved in metabolic and biosynthetic processes. We constructed a potential miRNA-gene regulatory network from the differentially expressed genes (DEGs) and DE miRNAs. Finally, 22 DEGs and nine DE miRNAs were selected for RT-qPCR validation. Of these, downregulation of six miRNAs, bta-miR-34c, bta-miR-122, bta-miR-195, bta-miR-449b, bta-miR-2425-5p, and bta-miR-2428-3p were confirmed; In addition, upregulation of 16 genes, ACSS2, DDIT4, INHBE, INSIG1, PNRC1, PSAT1, PSPH, PYCR1, SC4MOL, SLC34A2, SCD, SPARC, IDI1, PCK2, HMGCS1, and SMIM14 and downregulation of two genes, BATF3 and WFDC2 were confirmed. Specially, bta-miR-34c and bta-miR-449b potentially regulated PYCR1 and DDIT4, which were involved in cancer progression and angiogenesis. Our study presented for the first time the comprehensive miRNA and mRNA alterations in MDBK cells expressing the BPV E5 oncogene, providing new insights into the tumorigenesis induced by BPV E5.https://peerj.com/articles/8098.pdfBovine papillomavirusE5microRNAsRNA-seqTarget genesmiRNA-gene network |
| spellingShingle | Feng Pang Zhen Chen Chengqiang Wang Mengmeng Zhang Zhenxing Zhang Xiaohong Yang Yiying Zheng Ang Liu Yiwen Cheng Jie Chen Bin Li Li Du Fengyang Wang Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene PeerJ Bovine papillomavirus E5 microRNAs RNA-seq Target genes miRNA-gene network |
| title | Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene |
| title_full | Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene |
| title_fullStr | Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene |
| title_full_unstemmed | Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene |
| title_short | Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene |
| title_sort | comprehensive analysis of differentially expressed micrornas and mrnas in mdbk cells expressing bovine papillomavirus e5 oncogene |
| topic | Bovine papillomavirus E5 microRNAs RNA-seq Target genes miRNA-gene network |
| url | https://peerj.com/articles/8098.pdf |
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