The Antibiotic Resistance Profiling of Bacteria Isolated from Hospital Wastewater in Multan

Resistance against antibiotics is an emerging health crisis worldwide, mainly due to misuse and overuse of antibiotics. Production of the antibiotics released active antibiotic residues in the environment. Bacteria selecting these active antibiotic residues and the genes present in it and get resis...

Full description

Bibliographic Details
Main Authors: Saba Naeem, Iram Asim, Kashaf Junaid, Humaira Yasmeen
Format: Article
Language:English
Published: University of Management and Technology 2022-03-01
Series:Bioscientific Review
Subjects:
Online Access:https://journals.umt.edu.pk/index.php/BSR/article/view/1977
Description
Summary:Resistance against antibiotics is an emerging health crisis worldwide, mainly due to misuse and overuse of antibiotics. Production of the antibiotics released active antibiotic residues in the environment. Bacteria selecting these active antibiotic residues and the genes present in it and get resistance against antibiotics. The study was conducted to determine the prevalence of multidrug resistant bacterial strains isolated from hospital waste water. By using standard procedures bacterial resistance pattern against different classes of antibiotics and their species level identification were analysed. To determine the bacterial activity against the antimicrobial agents the disc diffusion method was used. The clear zones were measured separately in millimeter around each disc. Five wastewater samples were collected from different drainage regions of the hospitals in Multan. Total 45 bacterial strains were isolated. Out of 45 bacterial strains, 13 strains (29%) were resistant against two or more than two classes of antibiotics. From sample 2 and 3 all the isolated strains (100%) were MDR. Twenty-five bacterial strains (55.5%) belonged to Bacillus species and other were Enterococcus species, Micrococcus species, Staphylococcus species and Streptococcus species. Resistant bacterial strains in hospital wastes demands effectual treatment plants to treat waste before they dispose of into hospital waste lines. Keywords: antibiotics, antimicrobial activity, hospital wastewater, multi drug resistant bacteria, zone of inhibition Copyright (c_ The Authors
ISSN:2663-4198
2663-4201