Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations]
Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) b...
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F1000 Research Ltd
2017-11-01
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| Online Access: | https://f1000research.com/articles/6-1984/v1 |
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| author | Nursyirwani Nursyirwani Widya Asmara Agnesia Endang Tri Hastuti Wahyuni Triyanto Triyanto Muhammad Fauzi Zainal Abidin Muchlisin |
| author_facet | Nursyirwani Nursyirwani Widya Asmara Agnesia Endang Tri Hastuti Wahyuni Triyanto Triyanto Muhammad Fauzi Zainal Abidin Muchlisin |
| author_sort | Nursyirwani Nursyirwani |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10oC and 45oC, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae. |
| first_indexed | 2024-12-20T11:57:01Z |
| format | Article |
| id | doaj.art-17e313ccbb774eddb674045814153885 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-20T11:57:01Z |
| publishDate | 2017-11-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-17e313ccbb774eddb6740458141538852022-12-21T19:41:37ZengF1000 Research LtdF1000Research2046-14022017-11-01610.12688/f1000research.12734.113795Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations]Nursyirwani Nursyirwani0Widya Asmara1Agnesia Endang Tri Hastuti Wahyuni2Triyanto Triyanto3Muhammad Fauzi4Zainal Abidin Muchlisin5Faculty of Fishery and Marine Science, Universitas Riau, Pekanbaru, IndonesiaFaculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, IndonesiaFaculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, IndonesiaDepartment of Fisheries, Faculty of Agriculture, Gadjah Mada University, Yogyakarta, IndonesiaFaculty of Fishery and Marine Science, Universitas Riau, Pekanbaru, IndonesiaFaculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, IndonesiaLactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10oC and 45oC, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae.https://f1000research.com/articles/6-1984/v1Environmental MicrobiologyMarine & Freshwater Ecology |
| spellingShingle | Nursyirwani Nursyirwani Widya Asmara Agnesia Endang Tri Hastuti Wahyuni Triyanto Triyanto Muhammad Fauzi Zainal Abidin Muchlisin Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations] F1000Research Environmental Microbiology Marine & Freshwater Ecology |
| title | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations] |
| title_full | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations] |
| title_fullStr | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations] |
| title_full_unstemmed | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations] |
| title_short | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract [version 1; referees: 2 approved, 1 approved with reservations] |
| title_sort | phenotype and genotype of lactic acid bacteria lab isolated from the tiger grouper epinephelus fuscoguttatus alimentary tract version 1 referees 2 approved 1 approved with reservations |
| topic | Environmental Microbiology Marine & Freshwater Ecology |
| url | https://f1000research.com/articles/6-1984/v1 |
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