Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
Abstract We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07247-z |
| _version_ | 1819042275308601344 |
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| author | Robyn Plowright David J. Belton David L. Kaplan Carole C. Perry |
| author_facet | Robyn Plowright David J. Belton David L. Kaplan Carole C. Perry |
| author_sort | Robyn Plowright |
| collection | DOAJ |
| description | Abstract We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0–100%) from pure components and multiple (N = 10) XRD patterns were collected for each mixture. A linear relationship between the ratio of selected peak heights and the molar ratio was found. Using this method, the mineralising capabilities of three known hydroxyapatite binding peptides, CaP(S) STLPIPHEFSRE, CaP(V) VTKHLNQISQSY and CaP(H) SVSVGMKPSPRP, was compared. All three directed mineralisation towards hydroxyapatite in a peptide concentration dependent manner. CaP(V) was most effective at inducing hydroxyapatite formation at higher reagent levels (Ca2+ = 200 mM), as also seen with peptide-silk chimeric materials, whereas CaP(S) was most effective when lower concentrations of calcium (20 mM) and phosphate were used. The approach can be extended to investigate HAP mineralisation in the presence of any number of mineralisation promoters or inhibitors. |
| first_indexed | 2024-12-21T09:38:18Z |
| format | Article |
| id | doaj.art-17e3fc5403d54507a659756aed5305ec |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T09:38:18Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-17e3fc5403d54507a659756aed5305ec2022-12-21T19:08:33ZengNature PortfolioScientific Reports2045-23222017-08-01711910.1038/s41598-017-07247-zQuantifying the efficiency of Hydroxyapatite Mineralising PeptidesRobyn Plowright0David J. Belton1David L. Kaplan2Carole C. Perry3Biomolecular and Materials Interface Research Group, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Clifton LaneBiomolecular and Materials Interface Research Group, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Clifton LaneDepartment of Biomedical Engineering, Tufts UniversityBiomolecular and Materials Interface Research Group, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Clifton LaneAbstract We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0–100%) from pure components and multiple (N = 10) XRD patterns were collected for each mixture. A linear relationship between the ratio of selected peak heights and the molar ratio was found. Using this method, the mineralising capabilities of three known hydroxyapatite binding peptides, CaP(S) STLPIPHEFSRE, CaP(V) VTKHLNQISQSY and CaP(H) SVSVGMKPSPRP, was compared. All three directed mineralisation towards hydroxyapatite in a peptide concentration dependent manner. CaP(V) was most effective at inducing hydroxyapatite formation at higher reagent levels (Ca2+ = 200 mM), as also seen with peptide-silk chimeric materials, whereas CaP(S) was most effective when lower concentrations of calcium (20 mM) and phosphate were used. The approach can be extended to investigate HAP mineralisation in the presence of any number of mineralisation promoters or inhibitors.https://doi.org/10.1038/s41598-017-07247-z |
| spellingShingle | Robyn Plowright David J. Belton David L. Kaplan Carole C. Perry Quantifying the efficiency of Hydroxyapatite Mineralising Peptides Scientific Reports |
| title | Quantifying the efficiency of Hydroxyapatite Mineralising Peptides |
| title_full | Quantifying the efficiency of Hydroxyapatite Mineralising Peptides |
| title_fullStr | Quantifying the efficiency of Hydroxyapatite Mineralising Peptides |
| title_full_unstemmed | Quantifying the efficiency of Hydroxyapatite Mineralising Peptides |
| title_short | Quantifying the efficiency of Hydroxyapatite Mineralising Peptides |
| title_sort | quantifying the efficiency of hydroxyapatite mineralising peptides |
| url | https://doi.org/10.1038/s41598-017-07247-z |
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