Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus?
Introduction: Endothelial Dysfunction (ED) is an earliest pathological process of atherosclerosis. Endothelium regulates vascular tone, platelet activity, leukocyte adhesion and thrombosis. Impaired function of endothelium initiates the development of atherosclerosis. Nitric oxide is one of the most...
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| Format: | Article |
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JCDR Research and Publications Pvt. Ltd.
2017-12-01
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| Series: | National Journal of Laboratory Medicine |
| Subjects: |
| _version_ | 1818410541507411968 |
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| author | Siva Prasad Palem Philips Abraham Sachu Philip |
| author_facet | Siva Prasad Palem Philips Abraham Sachu Philip |
| author_sort | Siva Prasad Palem |
| collection | DOAJ |
| description | Introduction: Endothelial Dysfunction (ED) is an earliest pathological process of atherosclerosis. Endothelium regulates vascular tone, platelet activity, leukocyte adhesion and thrombosis. Impaired function of endothelium initiates the development of atherosclerosis. Nitric oxide is one of the most effective endogenous vasodilator and also a marker for ED.
Aim: To assess whether long term duration of diabetes is a factor to cause ED and its complications in patients with Type 2 diabetes mellitus. Hence, the study has been designed to assess the ED in patients with long term duration of Type 2 diabetes for early prediction of vascular complications.
Materials and Methods: The study was conducted on 47 Type 2 diabetic subjects. Among these 27 subjects with <5 years duration of diabetes (Group-I) and remaining 20 subjects with >5 years duration of diabetes (Group-II). Glucose, HbA1c, BMI and lipid profile were estimated by well established methods in auto-analyzer, MDA by Thiobarbituric Acid Reactive Substances (TBARS), total antioxidant capacity as Ferric Reducing Ability of Plasma (FRAP) and NO was estimated by kinetic cadmium reduction method using spectrophotometer. Statistical analysis was performed by “Kruskal-Wallis” test.
Result: Significantly low level of NO was identified in Type 2 diabetic patients with >5 years duration of disease compared to <5 years duration. MDA shows significantly high in >5 years duration of diabetes and no significant difference in the level of FRAP among the study groups. It has also shown significantly high level of age in >5 years duration of Type 2 diabetes than <5 years. But, no significant differences in the levels of HbA1c, lipid profile were identified between two study groups.
Conclusion: Age and oxidative stress (lipid peroxidation) has been recognized as risk factors for ED and future complications in patients with more than 5 years duration of Type 2 diabetes. |
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| language | English |
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| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-17e42a5fbacb4f1596ef0c8f22ed17c82022-12-21T23:06:46ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822017-12-0164BO07BO1210.7860/NJLM/2017/29048:2251Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus?Siva Prasad Palem0Philips Abraham1Sachu Philip2Assistant Professor/Lecturer, Department of Biochemistry, Annapoorana Medical College and Hospital, Salem, Tamil Nadu, India.Professor, Department of Biochemistry, Al-Azhar Medical College and Super Speciality Hospital, Thodupuzha, Idukki, Kerala, India.Reader, Department of Biochemistry, Vivekanada Dental College, Namakkal, Tamil Nadu, India.Introduction: Endothelial Dysfunction (ED) is an earliest pathological process of atherosclerosis. Endothelium regulates vascular tone, platelet activity, leukocyte adhesion and thrombosis. Impaired function of endothelium initiates the development of atherosclerosis. Nitric oxide is one of the most effective endogenous vasodilator and also a marker for ED. Aim: To assess whether long term duration of diabetes is a factor to cause ED and its complications in patients with Type 2 diabetes mellitus. Hence, the study has been designed to assess the ED in patients with long term duration of Type 2 diabetes for early prediction of vascular complications. Materials and Methods: The study was conducted on 47 Type 2 diabetic subjects. Among these 27 subjects with <5 years duration of diabetes (Group-I) and remaining 20 subjects with >5 years duration of diabetes (Group-II). Glucose, HbA1c, BMI and lipid profile were estimated by well established methods in auto-analyzer, MDA by Thiobarbituric Acid Reactive Substances (TBARS), total antioxidant capacity as Ferric Reducing Ability of Plasma (FRAP) and NO was estimated by kinetic cadmium reduction method using spectrophotometer. Statistical analysis was performed by “Kruskal-Wallis” test. Result: Significantly low level of NO was identified in Type 2 diabetic patients with >5 years duration of disease compared to <5 years duration. MDA shows significantly high in >5 years duration of diabetes and no significant difference in the level of FRAP among the study groups. It has also shown significantly high level of age in >5 years duration of Type 2 diabetes than <5 years. But, no significant differences in the levels of HbA1c, lipid profile were identified between two study groups. Conclusion: Age and oxidative stress (lipid peroxidation) has been recognized as risk factors for ED and future complications in patients with more than 5 years duration of Type 2 diabetes.Body mass indexFerric reducing ability of plasmaGlycosylated hemoglobinMalondialdehydeNitric oxide |
| spellingShingle | Siva Prasad Palem Philips Abraham Sachu Philip Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus? National Journal of Laboratory Medicine Body mass index Ferric reducing ability of plasma Glycosylated hemoglobin Malondialdehyde Nitric oxide |
| title | Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus? |
| title_full | Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus? |
| title_fullStr | Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus? |
| title_full_unstemmed | Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus? |
| title_short | Is Long Term Duration of Diabetes is a Factor to Cause Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus? |
| title_sort | is long term duration of diabetes is a factor to cause endothelial dysfunction in patients with type 2 diabetes mellitus |
| topic | Body mass index Ferric reducing ability of plasma Glycosylated hemoglobin Malondialdehyde Nitric oxide |
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