Molecular detection and characterization of <i>Hop stunt viroid</i> sequence variants from naturally infected pomegranate (<i>Punica granatum </i>L.) in Tunisia

Molecular detection and characterization of <i>Hop stunt viroid</i> sequence variants from naturally infected pomegranate (<i>Punica granatum </i>L.) in Tunisia

Tunisian pomegranate Hop stunt viroid (HSVd) variants are described. Dot-blot hybridization, S-Page, and reverse transcription polymerase chain reaction (RT-PCR) of RNA extracts from infected tissues were carried out. Results obtained by these techniques were confirmed by cDNA sequencing. The geneti...

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Bibliographic Details
Main Authors: Faten GORSANE, Amine ELLEUCH, Imène HAMDI, Amel SALHI-HANNACHI, Hatem FAKHFAKH
Format: Article
Language:English
Published: Firenze University Press 2010-09-01
Series:Phytopathologia Mediterranea
Subjects:
HSVd
sequence variability
phylogeny
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5339

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