The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study
ABSTRACTUpregulation of Sirtuin Type 1 (SIRT1), a nicotinamide adeninedinucleotide (NAD+)-dependent deacetylase, has been proved to protect against ample ocular diseases, while its effect on retinitis pigmentosa (RP) has not been illustrated. The study was aimed to explore the impacts of resveratrol...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2023.2226695 |
| _version_ | 1797361618505433088 |
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| author | Weiming Yan Yan Sun Yutong Wang Wangjiao Liang Yuxin Xia Weihua Yan Meizhu Chen Tao Chen Dongliang Li |
| author_facet | Weiming Yan Yan Sun Yutong Wang Wangjiao Liang Yuxin Xia Weihua Yan Meizhu Chen Tao Chen Dongliang Li |
| author_sort | Weiming Yan |
| collection | DOAJ |
| description | ABSTRACTUpregulation of Sirtuin Type 1 (SIRT1), a nicotinamide adeninedinucleotide (NAD+)-dependent deacetylase, has been proved to protect against ample ocular diseases, while its effect on retinitis pigmentosa (RP) has not been illustrated. The study was aimed to explore the impacts of resveratrol (RSV), a SIRT1 activator, on the photoreceptor degeneration in a rat model of RP induced by N-methyl-N-nitrosourea (MNU), an alkylation. The rats were induced RP phenotypes via the intraperitoneal injection of MNU. The electroretinogram was conducted and revealed that RSV could not prevent the decline of retinal function in the RP rats. The optical coherence tomography (OCT) and the retinal histological examination were performed and showed that the reduced thickness of the outer nuclear layer (ONL) was not preserved by RSV intervention. The immunostaining technique was applied. Afther the MNU administration, the number of the apoptotic photoreceptors in the ONL throughout the retinasand the number of microglia cells present among the outer part throughout the retinas were not significantly reduced by RSV. Western blotting was also performed. The data showed that the level of SIRT1 protein was decreased after MNU administration, while RSV was not able to obviously alleviate the downregulation. Our data together indicated that RSV was not able to rescue the photoreceptor degeneration in the MNU-induced RP rats, which might be due to the MNU-induced consumption of the NAD+. |
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| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-03-08T15:57:16Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-17e88d99ab2c4fc68c1d6703b0a71a752024-01-08T17:12:29ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852023-12-0127113814810.1080/19768354.2023.2226695The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo studyWeiming Yan0Yan Sun1Yutong Wang2Wangjiao Liang3Yuxin Xia4Weihua Yan5Meizhu Chen6Tao Chen7Dongliang Li8Department of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hopsital Affiliated to Xiamen University), Fuzhou, People’s Republic of ChinaDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hopsital Affiliated to Xiamen University), Fuzhou, People’s Republic of ChinaDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hopsital Affiliated to Xiamen University), Fuzhou, People’s Republic of ChinaDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hopsital Affiliated to Xiamen University), Fuzhou, People’s Republic of ChinaDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hopsital Affiliated to Xiamen University), Fuzhou, People’s Republic of ChinaChangtai No.2 High School of Fujian Province, Zhangzhou, People’s Republic of ChinaDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hopsital Affiliated to Xiamen University), Fuzhou, People’s Republic of ChinaCenter of Clinical Aerospace Medicine, Air Force Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Hepatobiliary Disease, The 900th Hospital of Joint Logistic Support Force, PLA, Fuzhou, People’s Republic of ChinaABSTRACTUpregulation of Sirtuin Type 1 (SIRT1), a nicotinamide adeninedinucleotide (NAD+)-dependent deacetylase, has been proved to protect against ample ocular diseases, while its effect on retinitis pigmentosa (RP) has not been illustrated. The study was aimed to explore the impacts of resveratrol (RSV), a SIRT1 activator, on the photoreceptor degeneration in a rat model of RP induced by N-methyl-N-nitrosourea (MNU), an alkylation. The rats were induced RP phenotypes via the intraperitoneal injection of MNU. The electroretinogram was conducted and revealed that RSV could not prevent the decline of retinal function in the RP rats. The optical coherence tomography (OCT) and the retinal histological examination were performed and showed that the reduced thickness of the outer nuclear layer (ONL) was not preserved by RSV intervention. The immunostaining technique was applied. Afther the MNU administration, the number of the apoptotic photoreceptors in the ONL throughout the retinasand the number of microglia cells present among the outer part throughout the retinas were not significantly reduced by RSV. Western blotting was also performed. The data showed that the level of SIRT1 protein was decreased after MNU administration, while RSV was not able to obviously alleviate the downregulation. Our data together indicated that RSV was not able to rescue the photoreceptor degeneration in the MNU-induced RP rats, which might be due to the MNU-induced consumption of the NAD+.https://www.tandfonline.com/doi/10.1080/19768354.2023.2226695Retinitis pigmentosaresveratrolalkylationelectroretinogramSIRT1 |
| spellingShingle | Weiming Yan Yan Sun Yutong Wang Wangjiao Liang Yuxin Xia Weihua Yan Meizhu Chen Tao Chen Dongliang Li The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study Animal Cells and Systems Retinitis pigmentosa resveratrol alkylation electroretinogram SIRT1 |
| title | The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study |
| title_full | The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study |
| title_fullStr | The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study |
| title_full_unstemmed | The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study |
| title_short | The impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation: an in-vivo study |
| title_sort | impacts of resveratrol on the retinal degeneration in a rat model of retinitis pigmentosa induced by alkylation an in vivo study |
| topic | Retinitis pigmentosa resveratrol alkylation electroretinogram SIRT1 |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2023.2226695 |
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