The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study
Background: to evaluate the effects of one week of supplementation with curcumin combined with piperine on physical performance, immune system cell counts, muscle damage, and plasma levels of inflammatory markers after a treadmill running training session. Methods: This study is a double-blind, cros...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/4/573 |
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| author | Stéfani Miranda-Castro Felipe J. Aidar Samara Silva de Moura Lucas Marcucci-Barbosa Lázaro Fernandes Lobo Francisco de Assis Dias Martins-Júnior Roberta da Silva Filha Pedro Alves Soares Vaz de Castro Ana Cristina Simões e Silva Danielle da Glória de Souza Siomara Aparecida da Silva Kelerson Mauro de Castro Pinto Guilherme de Paula Costa Ana Filipa Silva Filipe Manuel Clemente William Valadares Campos Pereira Albená Nunes-Silva |
| author_facet | Stéfani Miranda-Castro Felipe J. Aidar Samara Silva de Moura Lucas Marcucci-Barbosa Lázaro Fernandes Lobo Francisco de Assis Dias Martins-Júnior Roberta da Silva Filha Pedro Alves Soares Vaz de Castro Ana Cristina Simões e Silva Danielle da Glória de Souza Siomara Aparecida da Silva Kelerson Mauro de Castro Pinto Guilherme de Paula Costa Ana Filipa Silva Filipe Manuel Clemente William Valadares Campos Pereira Albená Nunes-Silva |
| author_sort | Stéfani Miranda-Castro |
| collection | DOAJ |
| description | Background: to evaluate the effects of one week of supplementation with curcumin combined with piperine on physical performance, immune system cell counts, muscle damage, and plasma levels of inflammatory markers after a treadmill running training session. Methods: This study is a double-blind, crossover-balanced clinical trial with a three-week intervention. Sixteen male runners with a mean age of 36 ± 9 years and VO2 max of 60.6 ± 9.03 mL.kg <sup>−1</sup> min <sup>−1</sup> were recruited and randomly divided into 2 groups: the first group (CPG) was supplemented daily for 7 days with 500 mg of curcumin + 20 mg piperine, and the second group (PG) was supplemented with 540 mg of cellulose. After the 7th day of supplementation, the volunteers participated in the experimental running protocol, where blood samples were collected before, after, and one hour after exercise for analysis of the number of leukocytes, creatine kinase, and cytokine concentration (IL-2, TNF-α, IFN, IL-6, and IL-10) using flow cytometry. This process was repeated, reversing the supplementation offered to the groups. Results: curcumin and piperine supplementation could not change the physical performance, immune cell counts, and muscle damage; however, the aerobic fatiguing exercise protocol inhibited the elevation of the plasmatic levels of some cytokines. The running exercise protocol could elevate the circulating levels of IL-2 (from 49.7 to 59.3 pg/mL), TNF-α (from 48.5 to 51.5 pg/mL), INF (from 128.8 to 165.0 pg/mL), IL-6 (from 63.1 to 77.3 pg/mL), and IL-10 (from 48.9 to 59.6 pg/mL) 1 h after the end of the running protocol. However, the curcumin and piperine supplementation could inhibit this elevation. Conclusions: curcumin and piperine supplementation had no effect on physical performance, immune cell counts, or muscle damage; however, the supplementation could modulate the kinetics of IL-2, TNF-α, INF, IL-6, and IL-10 1 h after the end of exercise. |
| first_indexed | 2024-03-09T11:07:49Z |
| format | Article |
| id | doaj.art-17e8c9f592684446a981fcfc601b5f88 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T11:07:49Z |
| publishDate | 2022-04-01 |
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| series | Biology |
| spelling | doaj.art-17e8c9f592684446a981fcfc601b5f882023-12-01T00:51:44ZengMDPI AGBiology2079-77372022-04-0111457310.3390/biology11040573The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover StudyStéfani Miranda-Castro0Felipe J. Aidar1Samara Silva de Moura2Lucas Marcucci-Barbosa3Lázaro Fernandes Lobo4Francisco de Assis Dias Martins-Júnior5Roberta da Silva Filha6Pedro Alves Soares Vaz de Castro7Ana Cristina Simões e Silva8Danielle da Glória de Souza9Siomara Aparecida da Silva10Kelerson Mauro de Castro Pinto11Guilherme de Paula Costa12Ana Filipa Silva13Filipe Manuel Clemente14William Valadares Campos Pereira15Albená Nunes-Silva16Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilDepartment of Physical Education, Federal University of Sergipe (UFS), São Cristóvão 49100-000, BrazilGraduate Health and Nutrition Program, Federal University of Ouro Preto (PPGSN/UFOP), Ouro Preto 35400-000, BrazilLaboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilLaboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilLaboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilInterdisciplinary Laboratory of Medical Investigation, Department of Pediatrics, Faculty of Medicine UFMG (LIIM/UFMG), Belo Horizonte 30130-100, BrazilInterdisciplinary Laboratory of Medical Investigation, Department of Pediatrics, Faculty of Medicine UFMG (LIIM/UFMG), Belo Horizonte 30130-100, BrazilInterdisciplinary Laboratory of Medical Investigation, Department of Pediatrics, Faculty of Medicine UFMG (LIIM/UFMG), Belo Horizonte 30130-100, BrazilDepartment of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, BrazilSports Teaching Methodology Laboratory, School of Physical Education, Federal University of Ouro Preto (LAMEES/EEF-UFOP), Ouro Preto 35400-000, BrazilLaboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilGraduate Program in Biological Sciences, Research Center in Biological Sciences, Federal University of Ouro Preto (CEBIOL), Ouro Preto 35400-000, BrazilSports and Leisure School, Polytechnic Institute of Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347 Viana do Castelo, PortugalSports and Leisure School, Polytechnic Institute of Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347 Viana do Castelo, PortugalLaboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilLaboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, BrazilBackground: to evaluate the effects of one week of supplementation with curcumin combined with piperine on physical performance, immune system cell counts, muscle damage, and plasma levels of inflammatory markers after a treadmill running training session. Methods: This study is a double-blind, crossover-balanced clinical trial with a three-week intervention. Sixteen male runners with a mean age of 36 ± 9 years and VO2 max of 60.6 ± 9.03 mL.kg <sup>−1</sup> min <sup>−1</sup> were recruited and randomly divided into 2 groups: the first group (CPG) was supplemented daily for 7 days with 500 mg of curcumin + 20 mg piperine, and the second group (PG) was supplemented with 540 mg of cellulose. After the 7th day of supplementation, the volunteers participated in the experimental running protocol, where blood samples were collected before, after, and one hour after exercise for analysis of the number of leukocytes, creatine kinase, and cytokine concentration (IL-2, TNF-α, IFN, IL-6, and IL-10) using flow cytometry. This process was repeated, reversing the supplementation offered to the groups. Results: curcumin and piperine supplementation could not change the physical performance, immune cell counts, and muscle damage; however, the aerobic fatiguing exercise protocol inhibited the elevation of the plasmatic levels of some cytokines. The running exercise protocol could elevate the circulating levels of IL-2 (from 49.7 to 59.3 pg/mL), TNF-α (from 48.5 to 51.5 pg/mL), INF (from 128.8 to 165.0 pg/mL), IL-6 (from 63.1 to 77.3 pg/mL), and IL-10 (from 48.9 to 59.6 pg/mL) 1 h after the end of the running protocol. However, the curcumin and piperine supplementation could inhibit this elevation. Conclusions: curcumin and piperine supplementation had no effect on physical performance, immune cell counts, or muscle damage; however, the supplementation could modulate the kinetics of IL-2, TNF-α, INF, IL-6, and IL-10 1 h after the end of exercise.https://www.mdpi.com/2079-7737/11/4/573curcumininflammationrunningdietary supplements |
| spellingShingle | Stéfani Miranda-Castro Felipe J. Aidar Samara Silva de Moura Lucas Marcucci-Barbosa Lázaro Fernandes Lobo Francisco de Assis Dias Martins-Júnior Roberta da Silva Filha Pedro Alves Soares Vaz de Castro Ana Cristina Simões e Silva Danielle da Glória de Souza Siomara Aparecida da Silva Kelerson Mauro de Castro Pinto Guilherme de Paula Costa Ana Filipa Silva Filipe Manuel Clemente William Valadares Campos Pereira Albená Nunes-Silva The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study Biology curcumin inflammation running dietary supplements |
| title | The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study |
| title_full | The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study |
| title_fullStr | The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study |
| title_full_unstemmed | The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study |
| title_short | The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study |
| title_sort | curcumin supplementation with piperine can influence the acute elevation of exercise induced cytokines double blind crossover study |
| topic | curcumin inflammation running dietary supplements |
| url | https://www.mdpi.com/2079-7737/11/4/573 |
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