Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity
Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2021-04-01
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| Series: | Comparative Cytogenetics |
| Online Access: | https://compcytogen.pensoft.net/article/60649/download/pdf/ |
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| author | Pun Yeesin Phichaya Buasriyot Sukhonthip Ditcharoen Patcharaporn Chaiyasan Chatmongkon Suwannapoom Sippakorn Juntaree Sitthisak Jantarat Sucheela Talumphai Marcelo de Bello Cioffi Thomas Liehr Alongklod Tanomtong Weerayuth Supiwong |
| author_facet | Pun Yeesin Phichaya Buasriyot Sukhonthip Ditcharoen Patcharaporn Chaiyasan Chatmongkon Suwannapoom Sippakorn Juntaree Sitthisak Jantarat Sucheela Talumphai Marcelo de Bello Cioffi Thomas Liehr Alongklod Tanomtong Weerayuth Supiwong |
| author_sort | Pun Yeesin |
| collection | DOAJ |
| description | Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus. |
| first_indexed | 2024-12-19T14:29:47Z |
| format | Article |
| id | doaj.art-17e8fe646c3943bc89017249f918cad8 |
| institution | Directory Open Access Journal |
| issn | 1993-078X |
| language | English |
| last_indexed | 2024-12-19T14:29:47Z |
| publishDate | 2021-04-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Comparative Cytogenetics |
| spelling | doaj.art-17e8fe646c3943bc89017249f918cad82022-12-21T20:17:29ZengPensoft PublishersComparative Cytogenetics1993-078X2021-04-0115211913610.3897/CompCytogen.v15i2.6064960649Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversityPun Yeesin0Phichaya Buasriyot1Sukhonthip Ditcharoen2Patcharaporn Chaiyasan3Chatmongkon Suwannapoom4Sippakorn Juntaree5Sitthisak Jantarat6Sucheela Talumphai7Marcelo de Bello Cioffi8Thomas Liehr9Alongklod Tanomtong10Weerayuth Supiwong11Prince of Songkla UniversityKhon Kaen UniversityKhon Kaen UniversityKhon Kaen UniversityUniversity of PhayaoKhon Kaen UniversityPrince of Songkla UniversityRoi Et Rajabhat UniversityUniversidade Federal de São CarlosFriedrich Schiller UniversityKhon Kaen UniversityKhon Kaen UniversityKaryotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus.https://compcytogen.pensoft.net/article/60649/download/pdf/ |
| spellingShingle | Pun Yeesin Phichaya Buasriyot Sukhonthip Ditcharoen Patcharaporn Chaiyasan Chatmongkon Suwannapoom Sippakorn Juntaree Sitthisak Jantarat Sucheela Talumphai Marcelo de Bello Cioffi Thomas Liehr Alongklod Tanomtong Weerayuth Supiwong Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity Comparative Cytogenetics |
| title | Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity |
| title_full | Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity |
| title_fullStr | Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity |
| title_full_unstemmed | Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity |
| title_short | Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity |
| title_sort | comparative study of four mystus species bagridae siluriformes from thailand insights into their karyotypic diversity |
| url | https://compcytogen.pensoft.net/article/60649/download/pdf/ |
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