Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens
Abstract Laboratory mice live in specific pathogen‐free (SPF) conditions, resulting in an immature immune system comparable to that of newborns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
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| Series: | Clinical and Translational Science |
| Online Access: | https://doi.org/10.1111/cts.13697 |
| _version_ | 1797347329175453696 |
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| author | Caroline Falkenberg Christina Bartholdy Janne Koch Martin Fitzner Toft Søren Skov Camilla Hartmann Friis Hansen Axel Kornerup Hansen |
| author_facet | Caroline Falkenberg Christina Bartholdy Janne Koch Martin Fitzner Toft Søren Skov Camilla Hartmann Friis Hansen Axel Kornerup Hansen |
| author_sort | Caroline Falkenberg |
| collection | DOAJ |
| description | Abstract Laboratory mice live in specific pathogen‐free (SPF) conditions, resulting in an immature immune system comparable to that of newborns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontrolled spread of infections and associated diseases, so research facilities should seek safer alternatives. We immunized laboratory mice with a cocktail of pathogens, which were inactivated by ultraviolet irradiation and mixed with the adjuvant AddaVax. This immunization resulted in a higher percentage of CD8+ effector memory T cells compared to untreated mice, although the response was not as robust as in pet shop mice. In a model of skin inflammation, pre‐immunization led to an increased skin inflammatory response compared to non‐immunized mice. All immunized mice seroconverted to the pathogens in the mixture, while none of the non‐immunized mice housed together seroconverted to the pathogens applied to the pre‐immunized mice. In conclusion, pre‐immunization of mice impacts the immune system, which includes increasing the levels of CD8+ effector memory T cells. |
| first_indexed | 2024-03-08T11:46:12Z |
| format | Article |
| id | doaj.art-17eb575ccf154419a5393edf5fb3c8b9 |
| institution | Directory Open Access Journal |
| issn | 1752-8054 1752-8062 |
| language | English |
| last_indexed | 2024-03-08T11:46:12Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Science |
| spelling | doaj.art-17eb575ccf154419a5393edf5fb3c8b92024-01-24T18:33:50ZengWileyClinical and Translational Science1752-80541752-80622024-01-01171n/an/a10.1111/cts.13697Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogensCaroline Falkenberg0Christina Bartholdy1Janne Koch2Martin Fitzner Toft3Søren Skov4Camilla Hartmann Friis Hansen5Axel Kornerup Hansen6Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C DenmarkTranslational Sciences, Research & Early Development, LEO Pharma A/S Ballerup DenmarkTranslational Sciences, Research & Early Development, LEO Pharma A/S Ballerup DenmarkQM Diagnostics Nijmegen The NetherlandsDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg C DenmarkAbstract Laboratory mice live in specific pathogen‐free (SPF) conditions, resulting in an immature immune system comparable to that of newborns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontrolled spread of infections and associated diseases, so research facilities should seek safer alternatives. We immunized laboratory mice with a cocktail of pathogens, which were inactivated by ultraviolet irradiation and mixed with the adjuvant AddaVax. This immunization resulted in a higher percentage of CD8+ effector memory T cells compared to untreated mice, although the response was not as robust as in pet shop mice. In a model of skin inflammation, pre‐immunization led to an increased skin inflammatory response compared to non‐immunized mice. All immunized mice seroconverted to the pathogens in the mixture, while none of the non‐immunized mice housed together seroconverted to the pathogens applied to the pre‐immunized mice. In conclusion, pre‐immunization of mice impacts the immune system, which includes increasing the levels of CD8+ effector memory T cells.https://doi.org/10.1111/cts.13697 |
| spellingShingle | Caroline Falkenberg Christina Bartholdy Janne Koch Martin Fitzner Toft Søren Skov Camilla Hartmann Friis Hansen Axel Kornerup Hansen Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens Clinical and Translational Science |
| title | Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens |
| title_full | Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens |
| title_fullStr | Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens |
| title_full_unstemmed | Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens |
| title_short | Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens |
| title_sort | induction of cd8 immune memory and enhanced inflammation in a skin inflammation model through pre immunization with inactivated pathogens |
| url | https://doi.org/10.1111/cts.13697 |
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