Study on species identification of Cronobacter spp. in foods
Objective To compare the species identification method of Cronobacter spp. in food, in order to establish rapid, high throughout identification method. Methods A total of 19 Cronobacter spp. in foods were identified with three method including biochemical method using VITEK 2.0 Compact, matrix assis...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The Editorial Office of Chinese Journal of Food Hygiene
2020-09-01
|
| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszzen/article/abstract/20200507 |
| _version_ | 1797769014048456704 |
|---|---|
| author | Hongzhi ZHANG Xuewei LIU Teng WEI Junyan LU Xi ZHANG |
| author_facet | Hongzhi ZHANG Xuewei LIU Teng WEI Junyan LU Xi ZHANG |
| author_sort | Hongzhi ZHANG |
| collection | DOAJ |
| description | Objective To compare the species identification method of Cronobacter spp. in food, in order to establish rapid, high throughout identification method. Methods A total of 19 Cronobacter spp. in foods were identified with three method including biochemical method using VITEK 2.0 Compact, matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and whole-genome sequencing (WGS). The advantages and disadvantages of the three identification method were analyzed. Results The result indicated that the accuracy of VITEK identification for C. sakazakii and C. malonaticus was 67% and 66% respectively. However, WGS and MALDI-TOF MS obtained the same result for 19 Cronobacter spp.. Three subspecies could not be identified by MALDI-TOF MS due to the limited database. Conclusion Biochemical method could not be used for species identification of Cronobacter spp. MALDI-TOF MS was rapid, high throughout and accurate, but still need to expand the fingerprint database for more Cronobacter spp. isolates. |
| first_indexed | 2024-03-12T21:02:41Z |
| format | Article |
| id | doaj.art-17ec80b288b5473ab2c02a513bb07fa5 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-12T21:02:41Z |
| publishDate | 2020-09-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-17ec80b288b5473ab2c02a513bb07fa52023-07-31T02:32:01ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562020-09-01320550350810.13590/j.cjfh.2020.05.0061004-8456(2020)05-0503-06Study on species identification of Cronobacter spp. in foodsHongzhi ZHANGXuewei LIUTeng WEIJunyan LUXi ZHANGObjective To compare the species identification method of Cronobacter spp. in food, in order to establish rapid, high throughout identification method. Methods A total of 19 Cronobacter spp. in foods were identified with three method including biochemical method using VITEK 2.0 Compact, matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and whole-genome sequencing (WGS). The advantages and disadvantages of the three identification method were analyzed. Results The result indicated that the accuracy of VITEK identification for C. sakazakii and C. malonaticus was 67% and 66% respectively. However, WGS and MALDI-TOF MS obtained the same result for 19 Cronobacter spp.. Three subspecies could not be identified by MALDI-TOF MS due to the limited database. Conclusion Biochemical method could not be used for species identification of Cronobacter spp. MALDI-TOF MS was rapid, high throughout and accurate, but still need to expand the fingerprint database for more Cronobacter spp. isolates.http://www.zgspws.com/zgspwszzen/article/abstract/20200507cronobacter spp.biochemical methodmatrix assisted laser desorption/ionization time of flight mass spectrometrywhole-genome sequencing |
| spellingShingle | Hongzhi ZHANG Xuewei LIU Teng WEI Junyan LU Xi ZHANG Study on species identification of Cronobacter spp. in foods Zhongguo shipin weisheng zazhi cronobacter spp. biochemical method matrix assisted laser desorption/ionization time of flight mass spectrometry whole-genome sequencing |
| title | Study on species identification of Cronobacter spp. in foods |
| title_full | Study on species identification of Cronobacter spp. in foods |
| title_fullStr | Study on species identification of Cronobacter spp. in foods |
| title_full_unstemmed | Study on species identification of Cronobacter spp. in foods |
| title_short | Study on species identification of Cronobacter spp. in foods |
| title_sort | study on species identification of cronobacter spp in foods |
| topic | cronobacter spp. biochemical method matrix assisted laser desorption/ionization time of flight mass spectrometry whole-genome sequencing |
| url | http://www.zgspws.com/zgspwszzen/article/abstract/20200507 |
| work_keys_str_mv | AT hongzhizhang studyonspeciesidentificationofcronobactersppinfoods AT xueweiliu studyonspeciesidentificationofcronobactersppinfoods AT tengwei studyonspeciesidentificationofcronobactersppinfoods AT junyanlu studyonspeciesidentificationofcronobactersppinfoods AT xizhang studyonspeciesidentificationofcronobactersppinfoods |