Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol
Changes in the metabolism of methionine can cause hyperhomocysteinemia, inducing a triad of atherosclerosis, hypertension, and increased oxidative stress. The generation of free radicals and oxidative damage to DNA is important in the liver damage caused by ethanol. In this study, the effect of meth...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2009-12-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400014 |
| _version_ | 1811272125057597440 |
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| author | Alceu Afonso Jordao Júnior Fernanda Aparecida Domenici Renata Cristina Lataro Guilherme Vannucchi Portari Helio Vannucchi |
| author_facet | Alceu Afonso Jordao Júnior Fernanda Aparecida Domenici Renata Cristina Lataro Guilherme Vannucchi Portari Helio Vannucchi |
| author_sort | Alceu Afonso Jordao Júnior |
| collection | DOAJ |
| description | Changes in the metabolism of methionine can cause hyperhomocysteinemia, inducing a triad of atherosclerosis, hypertension, and increased oxidative stress. The generation of free radicals and oxidative damage to DNA is important in the liver damage caused by ethanol. In this study, the effect of methionine overload associated or otherwise with acute administration of ethanol on homocysteine values, damage to DNA, lipoperoxidation and vitamin E was evaluated. Thirty rats were divided into 3 groups: Group Ethanol 24 hours (EG24), Group Methionine 24 hours (MG24), and Group Methionine and Ethanol 24 hours (MEG24). TBARS, vitamin E, GS and, homocysteine values were determined and the Comet assay was carried out. Increased GSH, vitamin E and homocysteine levels were observed for MEG24, and increased TBARS were observed in EG24. The Comet assay showed an increase in DNA damage in EG24 and DNA protection in MEG24. The administration of ethanol decreased antioxidant levels and increased TBARS, indicating the occurrence of oxidative stress with possible DNA damage. The combination of methionine and ethanol had a protective effect against the ethanol-induced damage, but increased the levels of homocysteine.<br>Alterações no metabolismo da metionina podem ocasionar hiper-homocisteinemia, quadro indutivo de aterosclerose, hipertensão e aumento do estresse oxidativo. A geração de radicais livres e dano oxidativo ao DNA são importantes na injúria hepática provocada pelo etanol. Neste estudo avaliaram-se os efeitos da sobrecarga de metionina associada ou não à administração aguda de etanol sobre valores de homocisteína, dano ao DNA, lipoperoxidação e vitamina E. Foram utilizados 30 ratos Wistar distribuídos em 3 Grupos: Grupo Etanol 24 horas (GE24), Grupo Metionina 24 horas (GM24) e Grupo Metionina e Etanol 24 horas (GME24). Realizaram-se determinações hepáticas de SRATB, vitamina E, GSH, homocisteína e Teste do Cometa e determinações plasmáticas de GSH e homocisteína. Valores aumentados de GSH, vitamina E e homocisteína foram observados para o GME24, e de SRATB no GE24. O Teste do Cometa mostrou aumento do dano ao DNA no GE24 e proteção ao DNA no GME24. A administração de etanol diminuiu os níveis de antioxidantes e aumentou o de SRATB, indicando ocorrência de estresse oxidativo, podendo ocasionar dano ao DNA. A presença da metionina associada com o etanol agiu como protetora contra os danos do etanol, mas aumentou os níveis de homocisteína. |
| first_indexed | 2024-04-12T22:33:33Z |
| format | Article |
| id | doaj.art-17ed813df1ac4be4944b6df11302d925 |
| institution | Directory Open Access Journal |
| issn | 1984-8250 2175-9790 |
| language | English |
| last_indexed | 2024-04-12T22:33:33Z |
| publishDate | 2009-12-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-17ed813df1ac4be4944b6df11302d9252022-12-22T03:13:54ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences1984-82502175-97902009-12-0145470971410.1590/S1984-82502009000400014Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanolAlceu Afonso Jordao JúniorFernanda Aparecida DomeniciRenata Cristina LataroGuilherme Vannucchi PortariHelio VannucchiChanges in the metabolism of methionine can cause hyperhomocysteinemia, inducing a triad of atherosclerosis, hypertension, and increased oxidative stress. The generation of free radicals and oxidative damage to DNA is important in the liver damage caused by ethanol. In this study, the effect of methionine overload associated or otherwise with acute administration of ethanol on homocysteine values, damage to DNA, lipoperoxidation and vitamin E was evaluated. Thirty rats were divided into 3 groups: Group Ethanol 24 hours (EG24), Group Methionine 24 hours (MG24), and Group Methionine and Ethanol 24 hours (MEG24). TBARS, vitamin E, GS and, homocysteine values were determined and the Comet assay was carried out. Increased GSH, vitamin E and homocysteine levels were observed for MEG24, and increased TBARS were observed in EG24. The Comet assay showed an increase in DNA damage in EG24 and DNA protection in MEG24. The administration of ethanol decreased antioxidant levels and increased TBARS, indicating the occurrence of oxidative stress with possible DNA damage. The combination of methionine and ethanol had a protective effect against the ethanol-induced damage, but increased the levels of homocysteine.<br>Alterações no metabolismo da metionina podem ocasionar hiper-homocisteinemia, quadro indutivo de aterosclerose, hipertensão e aumento do estresse oxidativo. A geração de radicais livres e dano oxidativo ao DNA são importantes na injúria hepática provocada pelo etanol. Neste estudo avaliaram-se os efeitos da sobrecarga de metionina associada ou não à administração aguda de etanol sobre valores de homocisteína, dano ao DNA, lipoperoxidação e vitamina E. Foram utilizados 30 ratos Wistar distribuídos em 3 Grupos: Grupo Etanol 24 horas (GE24), Grupo Metionina 24 horas (GM24) e Grupo Metionina e Etanol 24 horas (GME24). Realizaram-se determinações hepáticas de SRATB, vitamina E, GSH, homocisteína e Teste do Cometa e determinações plasmáticas de GSH e homocisteína. Valores aumentados de GSH, vitamina E e homocisteína foram observados para o GME24, e de SRATB no GE24. O Teste do Cometa mostrou aumento do dano ao DNA no GE24 e proteção ao DNA no GME24. A administração de etanol diminuiu os níveis de antioxidantes e aumentou o de SRATB, indicando ocorrência de estresse oxidativo, podendo ocasionar dano ao DNA. A presença da metionina associada com o etanol agiu como protetora contra os danos do etanol, mas aumentou os níveis de homocisteína.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400014MetioninaHomocisteínaRadicais LivresAntioxidantesEtanolMethionineHomocysteineFree RadicalsAntioxidantsEthanol |
| spellingShingle | Alceu Afonso Jordao Júnior Fernanda Aparecida Domenici Renata Cristina Lataro Guilherme Vannucchi Portari Helio Vannucchi Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol Brazilian Journal of Pharmaceutical Sciences Metionina Homocisteína Radicais Livres Antioxidantes Etanol Methionine Homocysteine Free Radicals Antioxidants Ethanol |
| title | Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol |
| title_full | Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol |
| title_fullStr | Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol |
| title_full_unstemmed | Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol |
| title_short | Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol |
| title_sort | effect of methionine load on homocysteine levels lipid peroxidation and dna damage in rats receiving ethanol |
| topic | Metionina Homocisteína Radicais Livres Antioxidantes Etanol Methionine Homocysteine Free Radicals Antioxidants Ethanol |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400014 |
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