Reliable Metabolic Flux Estimation in Escherichia coli Central Carbon Metabolism Using Intracellular Free Amino Acids

Reliable Metabolic Flux Estimation in Escherichia coli Central Carbon Metabolism Using Intracellular Free Amino Acids

13C metabolic flux analysis (MFA) is a tool of metabolic engineering for investigation of in vivo flux distribution. A direct 13C enrichment analysis of intracellular free amino acids (FAAs) is expected to reduce time for labeling experiments of the MFA. Measurable FAAs should, however, vary among t...

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Bibliographic Details
Main Authors: Nobuyuki Okahashi, Shuichi Kajihata, Chikara Furusawa, Hiroshi Shimizu
Format: Article
Language:English
Published: MDPI AG 2014-05-01
Series:Metabolites
Subjects:
metabolic flux analysis
intracellular free amino acids
Escherichia coli
GC-MS
Online Access:http://www.mdpi.com/2218-1989/4/2/408

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