The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis.
The E-cadherin-catenin complex plays an important role in the process of cell adhesion. Its dysfunction is associated with a decrease in cell differentiation and with increased invasiveness and metastasis. Our aim was to evaluate the expression of E-cadherin and B-catenin in advanced gastric cancer...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Via Medica
2010-06-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4257 |
| _version_ | 1818427709908320256 |
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| author | Marek Ustymowicz Katarzyna Guzińska-Ustymowicz Jolanta Czyzewska Anna Pryczynicz Andrzej Kemona |
| author_facet | Marek Ustymowicz Katarzyna Guzińska-Ustymowicz Jolanta Czyzewska Anna Pryczynicz Andrzej Kemona |
| author_sort | Marek Ustymowicz |
| collection | DOAJ |
| description | The E-cadherin-catenin complex plays an important role in the process of cell adhesion. Its dysfunction is associated with a decrease in cell differentiation and with increased invasiveness and metastasis. Our aim was to evaluate the expression of E-cadherin and B-catenin in advanced gastric cancer in relation to selected clinico-pathomorphological parameters. Formalin-fixed, paraffin-embedded tissue specimens were immunohistochemically stained with monoclonal antibodies E-cadherin (NCL-E-Cad, Novocastra Laboratiries Ltd; dilution 1:50), beta-catenin (NCL-B-CAT, Novocastra Laboratories Ltd; dilution 1:100), alpha-catenin (alpha-E-caten, Santa Cruz Biotechnology; dilution 1:300) and gamma-catenin (gamma-catenin, Santa Cruz Biotechnology; dilution 1:100). The expressions of E-cadherin and alpha-, beta-, gamma-catenins in the main mass of tumor and lymph node metastasis were investigated in 91 patients with gastric cancer. No statistically significant correlation was observed between the expressions of E-cadherin, alpha-, beta-catenins and histological differentiation and between the expressions of E-cadherin, alpha-, gamma-catenins and location or depth of invasion. Moreover, the expression of alpha-, gamma-catenins in the main mass of tumor was not associated with lymph node metastasis. However, we found a relationship between the expression of beta-catenin in the main mass of tumor and lymph node metastasis and tumor location. The depth of invasion was correlated with positive expression of beta-catenin in the main mass of gastric cancer. A statistically significant association was observed between the expressions of E-cadherin and beta-catenin in the main mass of tumor and lymph node involvement. The expression of alpha-catenin in the main mass of tumor was also associated with histological differentiation and Lauren's classification. Statistical analysis showed an association between the expression of E-cadherin and postoperative survival time. No significant correlation was found between the expression of alpha-, beta-, gamma-catenins and survival time. Our results may suggest that the E-cadherin-catenin complex is the factor indicative of metastasis and disease progression in gastric cancer. Also the expression of E-cadherin may play a role as a prognostic factor. |
| first_indexed | 2024-12-14T14:50:03Z |
| format | Article |
| id | doaj.art-17eee08b158a495699ffc5f20649414d |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-14T14:50:03Z |
| publishDate | 2010-06-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-17eee08b158a495699ffc5f20649414d2022-12-21T22:57:09ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312010-06-01481374510.5603/4257The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis.Marek UstymowiczKatarzyna Guzińska-UstymowiczJolanta CzyzewskaAnna PryczyniczAndrzej KemonaThe E-cadherin-catenin complex plays an important role in the process of cell adhesion. Its dysfunction is associated with a decrease in cell differentiation and with increased invasiveness and metastasis. Our aim was to evaluate the expression of E-cadherin and B-catenin in advanced gastric cancer in relation to selected clinico-pathomorphological parameters. Formalin-fixed, paraffin-embedded tissue specimens were immunohistochemically stained with monoclonal antibodies E-cadherin (NCL-E-Cad, Novocastra Laboratiries Ltd; dilution 1:50), beta-catenin (NCL-B-CAT, Novocastra Laboratories Ltd; dilution 1:100), alpha-catenin (alpha-E-caten, Santa Cruz Biotechnology; dilution 1:300) and gamma-catenin (gamma-catenin, Santa Cruz Biotechnology; dilution 1:100). The expressions of E-cadherin and alpha-, beta-, gamma-catenins in the main mass of tumor and lymph node metastasis were investigated in 91 patients with gastric cancer. No statistically significant correlation was observed between the expressions of E-cadherin, alpha-, beta-catenins and histological differentiation and between the expressions of E-cadherin, alpha-, gamma-catenins and location or depth of invasion. Moreover, the expression of alpha-, gamma-catenins in the main mass of tumor was not associated with lymph node metastasis. However, we found a relationship between the expression of beta-catenin in the main mass of tumor and lymph node metastasis and tumor location. The depth of invasion was correlated with positive expression of beta-catenin in the main mass of gastric cancer. A statistically significant association was observed between the expressions of E-cadherin and beta-catenin in the main mass of tumor and lymph node involvement. The expression of alpha-catenin in the main mass of tumor was also associated with histological differentiation and Lauren's classification. Statistical analysis showed an association between the expression of E-cadherin and postoperative survival time. No significant correlation was found between the expression of alpha-, beta-, gamma-catenins and survival time. Our results may suggest that the E-cadherin-catenin complex is the factor indicative of metastasis and disease progression in gastric cancer. Also the expression of E-cadherin may play a role as a prognostic factor.http://czasopisma.viamedica.pl/fhc/article/view/4257 |
| spellingShingle | Marek Ustymowicz Katarzyna Guzińska-Ustymowicz Jolanta Czyzewska Anna Pryczynicz Andrzej Kemona The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis. Folia Histochemica et Cytobiologica |
| title | The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis. |
| title_full | The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis. |
| title_fullStr | The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis. |
| title_full_unstemmed | The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis. |
| title_short | The expression of E-cadherin-catenin complex in patients with advanced gastric cancer: role in formation of metastasis. |
| title_sort | expression of e cadherin catenin complex in patients with advanced gastric cancer role in formation of metastasis |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4257 |
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