Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition
Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis. The identification of promoter elements responsible for SERK1 expression during the onset of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-11-01
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| Series: | Electronic Journal of Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345818300356 |
| _version_ | 1818067763001819136 |
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| author | Doribet Jiménez-Guillen Daniel Pérez-Pascual Ramón Souza-Perera Gregorio Godoy-Hernández José Juan Zúñiga-Aguilar |
| author_facet | Doribet Jiménez-Guillen Daniel Pérez-Pascual Ramón Souza-Perera Gregorio Godoy-Hernández José Juan Zúñiga-Aguilar |
| author_sort | Doribet Jiménez-Guillen |
| collection | DOAJ |
| description | Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis. The identification of promoter elements responsible for SERK1 expression during the onset of somatic embryogenesis can be useful to understand the molecular regulation of the cell-to embryo transition, and these promoter elements represent biotechnological tools in plant organ tissue culture. Results: A −1,620 bp DNA sequence located upstream of the Coffea canephora SERK1 gene homologue (CcSERK1) was isolated, and then, different segments containing key response elements (REs) for somatic embryogenesis onset and development were fused to the uidA (encoding a β-glucuronidase, GUS) reporter gene to evaluate its expression in transgenic leaf explants. DNA segments of −1,620 and −1048 bp in length directed uidA expression with patterns in leaf explants similar to those occurring during somatic embryogenesis. When a −792-bp fragment was used, uidA expression disappeared only in leaf explants and pro-embryogenic mass but persisted in developing embryos. No uidA expression was detected in any embryogenic stage when a −618-bp fragment was used. Conclusion: DNA deletions showed that a −1048-bp sequence located upstream of the CcSERK1 gene is sufficient to direct gene expression during the onset and the development of C. canephora somatic embryogenesis. The DNA segment located between −1048 and −792 bp (containing BBM and WUS REs) is needed for gene expression before embryogenesis onset but not during embryo development. The promoter segment between −792 and −618 bp (including GATA, ARR1AT, and ANT REs) regulates gene expression in developing embryos.How to cite: Jiménez-Guillen D, Pérez-Pascual D, Souza-Perera R, et al. Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition. Electron J Biotechnol 2018;36.https://doi.org/10.1016/j.ejbt.2018.08.005. Keywords: Biotechnological tool, Cell-to-embryo transition, Coffea canephora, Coffee, Gene expression, Plant organ tissue culture, Promoter functional analysis, Reporter genes, Somatic embryogenesis, Transgenic leaf explants, uidA |
| first_indexed | 2024-12-10T15:28:51Z |
| format | Article |
| id | doaj.art-17ef523e28274e6fbbb6105ece0c3129 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-10T15:28:51Z |
| publishDate | 2018-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-17ef523e28274e6fbbb6105ece0c31292022-12-22T01:43:28ZengElsevierElectronic Journal of Biotechnology0717-34582018-11-01363446Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transitionDoribet Jiménez-Guillen0Daniel Pérez-Pascual1Ramón Souza-Perera2Gregorio Godoy-Hernández3José Juan Zúñiga-Aguilar4Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A. C., Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A. C., Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A. C., Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A. C., Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, MexicoInstituto Tecnológico Superior de los Ríos, Km 3 carretera Balancán-Villahermosa, Tabasco 86930, Mexico; Corresponding author.Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis. The identification of promoter elements responsible for SERK1 expression during the onset of somatic embryogenesis can be useful to understand the molecular regulation of the cell-to embryo transition, and these promoter elements represent biotechnological tools in plant organ tissue culture. Results: A −1,620 bp DNA sequence located upstream of the Coffea canephora SERK1 gene homologue (CcSERK1) was isolated, and then, different segments containing key response elements (REs) for somatic embryogenesis onset and development were fused to the uidA (encoding a β-glucuronidase, GUS) reporter gene to evaluate its expression in transgenic leaf explants. DNA segments of −1,620 and −1048 bp in length directed uidA expression with patterns in leaf explants similar to those occurring during somatic embryogenesis. When a −792-bp fragment was used, uidA expression disappeared only in leaf explants and pro-embryogenic mass but persisted in developing embryos. No uidA expression was detected in any embryogenic stage when a −618-bp fragment was used. Conclusion: DNA deletions showed that a −1048-bp sequence located upstream of the CcSERK1 gene is sufficient to direct gene expression during the onset and the development of C. canephora somatic embryogenesis. The DNA segment located between −1048 and −792 bp (containing BBM and WUS REs) is needed for gene expression before embryogenesis onset but not during embryo development. The promoter segment between −792 and −618 bp (including GATA, ARR1AT, and ANT REs) regulates gene expression in developing embryos.How to cite: Jiménez-Guillen D, Pérez-Pascual D, Souza-Perera R, et al. Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition. Electron J Biotechnol 2018;36.https://doi.org/10.1016/j.ejbt.2018.08.005. Keywords: Biotechnological tool, Cell-to-embryo transition, Coffea canephora, Coffee, Gene expression, Plant organ tissue culture, Promoter functional analysis, Reporter genes, Somatic embryogenesis, Transgenic leaf explants, uidAhttp://www.sciencedirect.com/science/article/pii/S0717345818300356 |
| spellingShingle | Doribet Jiménez-Guillen Daniel Pérez-Pascual Ramón Souza-Perera Gregorio Godoy-Hernández José Juan Zúñiga-Aguilar Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition Electronic Journal of Biotechnology |
| title | Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition |
| title_full | Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition |
| title_fullStr | Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition |
| title_full_unstemmed | Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition |
| title_short | Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition |
| title_sort | cloning of the coffea canephora serk1 promoter and its molecular analysis during the cell to embryo transition |
| url | http://www.sciencedirect.com/science/article/pii/S0717345818300356 |
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