Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions
DnaJ proteins or heat shock protein 40s (HSP40s) form one of the largest heat shock protein families. In this study, 2 cDNAs encoding Nile tilapia (<i>Oreochromis niloticus</i>) DnaJ proteins (On-DnaJ B9b and On-DnaJ C3a) were successfully cloned and characterized. The structures and org...
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MDPI AG
2021-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/10/1509 |
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| author | Prapansak Srisapoome Kubpaphas Thummabancha Ratree Wongpanya |
| author_facet | Prapansak Srisapoome Kubpaphas Thummabancha Ratree Wongpanya |
| author_sort | Prapansak Srisapoome |
| collection | DOAJ |
| description | DnaJ proteins or heat shock protein 40s (HSP40s) form one of the largest heat shock protein families. In this study, 2 cDNAs encoding Nile tilapia (<i>Oreochromis niloticus</i>) DnaJ proteins (On-DnaJ B9b and On-DnaJ C3a) were successfully cloned and characterized. The structures and organizations of these two genes are first reported in the present study. <i>On-DnaJ B9b</i> is approximately 2.1 kb long and contains 2 exons and 1 intron, while <i>On-DnaJ C3a</i> is approximately 12 kb long and contains 12 exons and 11 introns. Under normal conditions, <i>On-DnaJ B9b</i> mRNA is highly expressed in gonad and trunk kidney tissues, while <i>On-DnaJ C3a</i> transcripts are abundantly expressed in gills, intestine, liver, and trunk kidney tissues. Following pathogenic infections, the expression of both genes is induced in the liver, spleen and head kidney tissues of Nile tilapia that were infected with two virulent pathogenic bacteria, <i>Streptococcus agalactiae</i> and <i>Flavobacterium columnare</i>. Silencing of these two genes was first carried out, and the results clearly indicated their crucial roles under both heat and bacterial stress conditions. The fundamental knowledge obtained from this study indicates the characteristic basic biofunctions of heat shock proteins in the regulation of intracellular proteins during infection, which involve preventing protein aggregation, promoting protein refolding, and activating unfolded protein degradation. |
| first_indexed | 2024-03-10T06:42:07Z |
| format | Article |
| id | doaj.art-17f4b37102ae4908af47f4932afa889b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T06:42:07Z |
| publishDate | 2021-10-01 |
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| series | Biomolecules |
| spelling | doaj.art-17f4b37102ae4908af47f4932afa889b2023-11-22T17:34:42ZengMDPI AGBiomolecules2218-273X2021-10-011110150910.3390/biom11101509Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress ConditionsPrapansak Srisapoome0Kubpaphas Thummabancha1Ratree Wongpanya2Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandLaboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandDnaJ proteins or heat shock protein 40s (HSP40s) form one of the largest heat shock protein families. In this study, 2 cDNAs encoding Nile tilapia (<i>Oreochromis niloticus</i>) DnaJ proteins (On-DnaJ B9b and On-DnaJ C3a) were successfully cloned and characterized. The structures and organizations of these two genes are first reported in the present study. <i>On-DnaJ B9b</i> is approximately 2.1 kb long and contains 2 exons and 1 intron, while <i>On-DnaJ C3a</i> is approximately 12 kb long and contains 12 exons and 11 introns. Under normal conditions, <i>On-DnaJ B9b</i> mRNA is highly expressed in gonad and trunk kidney tissues, while <i>On-DnaJ C3a</i> transcripts are abundantly expressed in gills, intestine, liver, and trunk kidney tissues. Following pathogenic infections, the expression of both genes is induced in the liver, spleen and head kidney tissues of Nile tilapia that were infected with two virulent pathogenic bacteria, <i>Streptococcus agalactiae</i> and <i>Flavobacterium columnare</i>. Silencing of these two genes was first carried out, and the results clearly indicated their crucial roles under both heat and bacterial stress conditions. The fundamental knowledge obtained from this study indicates the characteristic basic biofunctions of heat shock proteins in the regulation of intracellular proteins during infection, which involve preventing protein aggregation, promoting protein refolding, and activating unfolded protein degradation.https://www.mdpi.com/2218-273X/11/10/1509molecular chaperoneheat shock proteinHsp40aquaculture<i>Streptococcosis</i>columnaris |
| spellingShingle | Prapansak Srisapoome Kubpaphas Thummabancha Ratree Wongpanya Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions Biomolecules molecular chaperone heat shock protein Hsp40 aquaculture <i>Streptococcosis</i> columnaris |
| title | Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions |
| title_full | Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions |
| title_fullStr | Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions |
| title_full_unstemmed | Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions |
| title_short | Molecular Characterization and Defense Functions of the Nile Tilapia (<i>Oreochromis niloticus</i>) <i>DnaJ B9b</i> and <i>DnaJ C3a</i> Genes in Response to Pathogenic Bacteria under High-Temperature Stress Conditions |
| title_sort | molecular characterization and defense functions of the nile tilapia i oreochromis niloticus i i dnaj b9b i and i dnaj c3a i genes in response to pathogenic bacteria under high temperature stress conditions |
| topic | molecular chaperone heat shock protein Hsp40 aquaculture <i>Streptococcosis</i> columnaris |
| url | https://www.mdpi.com/2218-273X/11/10/1509 |
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