Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants
Nanoencapsulation with proteoliposomes from natural membranes has been proposed as a carrier for the highly efficient delivery of mineral nutrients into plant tissues. Since Boron deficiency occurred frequently in crops, and is an element with low movement in tissues, in this work, nanoencapsulated...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/4/1788 |
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| author | Juan Nicolas-Espinosa Pablo Garcia-Gomez Juan J. Rios Abel Piqueras Gloria Bárzana Micaela Carvajal |
| author_facet | Juan Nicolas-Espinosa Pablo Garcia-Gomez Juan J. Rios Abel Piqueras Gloria Bárzana Micaela Carvajal |
| author_sort | Juan Nicolas-Espinosa |
| collection | DOAJ |
| description | Nanoencapsulation with proteoliposomes from natural membranes has been proposed as a carrier for the highly efficient delivery of mineral nutrients into plant tissues. Since Boron deficiency occurred frequently in crops, and is an element with low movement in tissues, in this work, nanoencapsulated B vs free B was applied to in vitro sweet potato plants to investigate the regulation of B transporters (aquaporins and specific transporters). Additionally, an metabolomic analysis was performed, and mineral nutrient and pigment concentrations were determined. The results showed high increases in B concentration in leaves when B was applied as encapsulated, but also Fe and Mn concentration increased. Likewise, the metabolomics study showed that single carbohydrates of these plants could be related to the energy need for increasing the expression of most NIP aquaporins (NIP1;2, NIP1;3; NIP4;1, NIP4;2, NIP5;1, NIP6;1, and NIP7) and boron transporters (BOR2, BOR4 and BOR7;1). Therefore, the results were associated with the higher mobility of encapsulated B into leaves and the stimulation of transport into cells, since after applying encapsulated B, the aforementioned NIPs and BORs increased in expression. |
| first_indexed | 2024-03-09T22:43:14Z |
| format | Article |
| id | doaj.art-17f6fca4902a448c935dddb03fe57fc8 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T22:43:14Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-17f6fca4902a448c935dddb03fe57fc82023-11-23T18:34:11ZengMDPI AGApplied Sciences2076-34172022-02-01124178810.3390/app12041788Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> PlantsJuan Nicolas-Espinosa0Pablo Garcia-Gomez1Juan J. Rios2Abel Piqueras3Gloria Bárzana4Micaela Carvajal5Group of Aquaporins, Plant Nutrition Department, Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, 30100 Murcia, SpainGroup of Aquaporins, Plant Nutrition Department, Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, 30100 Murcia, SpainDepartment of Plant Physiology, Faculty of Sciences, University of Granada, 18071 Granada, SpainPlant Breeding Department, Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, 30100 Murcia, SpainGroup of Aquaporins, Plant Nutrition Department, Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, 30100 Murcia, SpainGroup of Aquaporins, Plant Nutrition Department, Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, 30100 Murcia, SpainNanoencapsulation with proteoliposomes from natural membranes has been proposed as a carrier for the highly efficient delivery of mineral nutrients into plant tissues. Since Boron deficiency occurred frequently in crops, and is an element with low movement in tissues, in this work, nanoencapsulated B vs free B was applied to in vitro sweet potato plants to investigate the regulation of B transporters (aquaporins and specific transporters). Additionally, an metabolomic analysis was performed, and mineral nutrient and pigment concentrations were determined. The results showed high increases in B concentration in leaves when B was applied as encapsulated, but also Fe and Mn concentration increased. Likewise, the metabolomics study showed that single carbohydrates of these plants could be related to the energy need for increasing the expression of most NIP aquaporins (NIP1;2, NIP1;3; NIP4;1, NIP4;2, NIP5;1, NIP6;1, and NIP7) and boron transporters (BOR2, BOR4 and BOR7;1). Therefore, the results were associated with the higher mobility of encapsulated B into leaves and the stimulation of transport into cells, since after applying encapsulated B, the aforementioned NIPs and BORs increased in expression.https://www.mdpi.com/2076-3417/12/4/1788aquaporinsboronboron transportersmetabolomic analysismineral nutritionin vitro culture |
| spellingShingle | Juan Nicolas-Espinosa Pablo Garcia-Gomez Juan J. Rios Abel Piqueras Gloria Bárzana Micaela Carvajal Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants Applied Sciences aquaporins boron boron transporters metabolomic analysis mineral nutrition in vitro culture |
| title | Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants |
| title_full | Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants |
| title_fullStr | Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants |
| title_full_unstemmed | Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants |
| title_short | Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro <i>Ipomoea batatas</i> Plants |
| title_sort | nanoencapsulated boron foliar supply increased expression of nips aquaporins and bor transporters of in vitro i ipomoea batatas i plants |
| topic | aquaporins boron boron transporters metabolomic analysis mineral nutrition in vitro culture |
| url | https://www.mdpi.com/2076-3417/12/4/1788 |
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