Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs
ABSTRACT Objective: the hernial sac is typically a bilayer structure consisting of connective tissue lined underneath by peritoneum. Some incidental findings on it, like smooth muscle cells and heterotopic ossification, can be explained as the end-stage metaplasia from undifferentiated cells. This...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Cirurgiões
2020-11-01
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| Series: | Revista do Colégio Brasileiro de Cirurgiões |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912020000100504&tlng=en |
| _version_ | 1831714188684165120 |
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| author | RENATO MIRANDA DE MELO ROBERPAULO ANACLETO NEVES LILIANA BORGES DE MENEZES |
| author_facet | RENATO MIRANDA DE MELO ROBERPAULO ANACLETO NEVES LILIANA BORGES DE MENEZES |
| author_sort | RENATO MIRANDA DE MELO |
| collection | DOAJ |
| description | ABSTRACT Objective: the hernial sac is typically a bilayer structure consisting of connective tissue lined underneath by peritoneum. Some incidental findings on it, like smooth muscle cells and heterotopic ossification, can be explained as the end-stage metaplasia from undifferentiated cells. This study aimed to search for mesenchymal stem cells in the incisional hernial sac by an immuno-histochemistry screening test. Methods: fifteen specimens of them were submitted to histochemistry analysis using CD133 monoclonal antibodies, a specific marker of mesenchymal stem cells. The biopsies were obtained from patients submitted to pure tissue repair for incisional hernias - the transposition with the hernial sac technique (Lázaro da Silva, 1971). Results: two-thirds of the specimens expressed on average 20 (twenty) CD133+ cells in each one. Conclusion: despite the screening nature of this study, the hernial sac may be considered a source of stem cells. This could explain those abnormal findings, and perhaps the induction of new fibroblasts in procedures that use it to optimize wound healing. |
| first_indexed | 2024-12-20T23:54:55Z |
| format | Article |
| id | doaj.art-17f84a26852246808021efc078002dd1 |
| institution | Directory Open Access Journal |
| issn | 1809-4546 |
| language | English |
| last_indexed | 2024-12-20T23:54:55Z |
| publishDate | 2020-11-01 |
| publisher | Colégio Brasileiro de Cirurgiões |
| record_format | Article |
| series | Revista do Colégio Brasileiro de Cirurgiões |
| spelling | doaj.art-17f84a26852246808021efc078002dd12022-12-21T19:22:45ZengColégio Brasileiro de CirurgiõesRevista do Colégio Brasileiro de Cirurgiões1809-45462020-11-014710.1590/0100-6991e-20202636Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairsRENATO MIRANDA DE MELOhttps://orcid.org/0000-0001-5230-2789ROBERPAULO ANACLETO NEVEShttps://orcid.org/0000-0003-1181-2373LILIANA BORGES DE MENEZEShttps://orcid.org/0000-0003-4237-6279ABSTRACT Objective: the hernial sac is typically a bilayer structure consisting of connective tissue lined underneath by peritoneum. Some incidental findings on it, like smooth muscle cells and heterotopic ossification, can be explained as the end-stage metaplasia from undifferentiated cells. This study aimed to search for mesenchymal stem cells in the incisional hernial sac by an immuno-histochemistry screening test. Methods: fifteen specimens of them were submitted to histochemistry analysis using CD133 monoclonal antibodies, a specific marker of mesenchymal stem cells. The biopsies were obtained from patients submitted to pure tissue repair for incisional hernias - the transposition with the hernial sac technique (Lázaro da Silva, 1971). Results: two-thirds of the specimens expressed on average 20 (twenty) CD133+ cells in each one. Conclusion: despite the screening nature of this study, the hernial sac may be considered a source of stem cells. This could explain those abnormal findings, and perhaps the induction of new fibroblasts in procedures that use it to optimize wound healing.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912020000100504&tlng=enStem CellsPeritoneumReconstructive Surgical ProceduresAbdominal Wall |
| spellingShingle | RENATO MIRANDA DE MELO ROBERPAULO ANACLETO NEVES LILIANA BORGES DE MENEZES Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs Revista do Colégio Brasileiro de Cirurgiões Stem Cells Peritoneum Reconstructive Surgical Procedures Abdominal Wall |
| title | Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs |
| title_full | Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs |
| title_fullStr | Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs |
| title_full_unstemmed | Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs |
| title_short | Tracking stem cells in the incisional hernial sac: a gaze beyond pure tissue repairs |
| title_sort | tracking stem cells in the incisional hernial sac a gaze beyond pure tissue repairs |
| topic | Stem Cells Peritoneum Reconstructive Surgical Procedures Abdominal Wall |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912020000100504&tlng=en |
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