A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements
Watermelon fruits of different ploidy levels exhibit significant variations in texture. This study aimed to investigate the primary factors that influence texture differences. To achieve this, we conducted an investigation into the differences in fruit texture development using homozygous autogamous...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/10/2/112 |
| _version_ | 1797298058361307136 |
|---|---|
| author | Xuqiang Lu Nan He Muhammad Anees Dongdong Yang Weikang Kong Jiwen Zhang Luming Yuan Xiaowen Luo Hongju Zhu Wenge Liu |
| author_facet | Xuqiang Lu Nan He Muhammad Anees Dongdong Yang Weikang Kong Jiwen Zhang Luming Yuan Xiaowen Luo Hongju Zhu Wenge Liu |
| author_sort | Xuqiang Lu |
| collection | DOAJ |
| description | Watermelon fruits of different ploidy levels exhibit significant variations in texture. This study aimed to investigate the primary factors that influence texture differences. To achieve this, we conducted an investigation into the differences in fruit texture development using homozygous autogamous diploid (2×) lines and their autotriploid (3×) and autotetraploid (4×) lines ‘Yixuan’. The study investigated fruit development, flesh texture profile analysis (TPA), flesh cell wall polysaccharide content, and flesh cell microstructure analysis in 2×, 3×, and 4× watermelon fruits. The study found that as watermelon fruits matured, several characteristics increased, including fruit weight, TSS (total soluble solids) content, rind hardness, flesh cell size, and cell wall polysaccharide contents such as crude fiber, cellulose, hemicellulose, lignin, and protopectin. In contrast, the flesh texture parameters and soluble pectin content of the flesh cell wall decreased over time. Significant differences in fruit flesh texture and flesh cell structure were observed among fruits of different ploidy levels at the 32 DAP. Specifically, the 2× fruits displayed considerably lower rind hardness, reduced flesh texture (Hardness, Fracturability, Chewiness, Gumminess), and cell density compared to the 3× and 4× fruits. Additionally, the 2× fruits had larger cell sizes than the 3× and 4× fruits. However, there were no significant differences observed in the flesh cell wall polysaccharide contents across various ploidy levels. These findings suggest that the variation in texture among watermelon fruits of different ploidy levels can be attributed to the size and arrangement of the flesh cells. This research provides a foundation for the further exploration of the intrinsic regulatory factors and molecular mechanisms contributing to texture variation in polyploid watermelon fruits. |
| first_indexed | 2024-03-07T22:30:17Z |
| format | Article |
| id | doaj.art-17fcb1c0e02f4b60ad220456c21e6333 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-07T22:30:17Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-17fcb1c0e02f4b60ad220456c21e63332024-02-23T15:18:35ZengMDPI AGHorticulturae2311-75242024-01-0110211210.3390/horticulturae10020112A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall MeasurementsXuqiang Lu0Nan He1Muhammad Anees2Dongdong Yang3Weikang Kong4Jiwen Zhang5Luming Yuan6Xiaowen Luo7Hongju Zhu8Wenge Liu9National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaWatermelon fruits of different ploidy levels exhibit significant variations in texture. This study aimed to investigate the primary factors that influence texture differences. To achieve this, we conducted an investigation into the differences in fruit texture development using homozygous autogamous diploid (2×) lines and their autotriploid (3×) and autotetraploid (4×) lines ‘Yixuan’. The study investigated fruit development, flesh texture profile analysis (TPA), flesh cell wall polysaccharide content, and flesh cell microstructure analysis in 2×, 3×, and 4× watermelon fruits. The study found that as watermelon fruits matured, several characteristics increased, including fruit weight, TSS (total soluble solids) content, rind hardness, flesh cell size, and cell wall polysaccharide contents such as crude fiber, cellulose, hemicellulose, lignin, and protopectin. In contrast, the flesh texture parameters and soluble pectin content of the flesh cell wall decreased over time. Significant differences in fruit flesh texture and flesh cell structure were observed among fruits of different ploidy levels at the 32 DAP. Specifically, the 2× fruits displayed considerably lower rind hardness, reduced flesh texture (Hardness, Fracturability, Chewiness, Gumminess), and cell density compared to the 3× and 4× fruits. Additionally, the 2× fruits had larger cell sizes than the 3× and 4× fruits. However, there were no significant differences observed in the flesh cell wall polysaccharide contents across various ploidy levels. These findings suggest that the variation in texture among watermelon fruits of different ploidy levels can be attributed to the size and arrangement of the flesh cells. This research provides a foundation for the further exploration of the intrinsic regulatory factors and molecular mechanisms contributing to texture variation in polyploid watermelon fruits.https://www.mdpi.com/2311-7524/10/2/112watermelontextureploidycell wall polysaccharidehistology |
| spellingShingle | Xuqiang Lu Nan He Muhammad Anees Dongdong Yang Weikang Kong Jiwen Zhang Luming Yuan Xiaowen Luo Hongju Zhu Wenge Liu A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements Horticulturae watermelon texture ploidy cell wall polysaccharide histology |
| title | A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements |
| title_full | A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements |
| title_fullStr | A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements |
| title_full_unstemmed | A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements |
| title_short | A Comparison of Watermelon Flesh Texture across Different Ploidy Levels Using Histology and Cell Wall Measurements |
| title_sort | comparison of watermelon flesh texture across different ploidy levels using histology and cell wall measurements |
| topic | watermelon texture ploidy cell wall polysaccharide histology |
| url | https://www.mdpi.com/2311-7524/10/2/112 |
| work_keys_str_mv | AT xuqianglu acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT nanhe acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT muhammadanees acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT dongdongyang acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT weikangkong acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT jiwenzhang acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT lumingyuan acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT xiaowenluo acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT hongjuzhu acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT wengeliu acomparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT xuqianglu comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT nanhe comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT muhammadanees comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT dongdongyang comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT weikangkong comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT jiwenzhang comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT lumingyuan comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT xiaowenluo comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT hongjuzhu comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements AT wengeliu comparisonofwatermelonfleshtextureacrossdifferentploidylevelsusinghistologyandcellwallmeasurements |