Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19
Abstract Background Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their asso...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-023-00568-x |
| _version_ | 1797577084386672640 |
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| author | Zahra Bagheri-Hosseinabadi Ali Shamsizadeh Fatemeh Bahrehmand Mitra Abbasifard |
| author_facet | Zahra Bagheri-Hosseinabadi Ali Shamsizadeh Fatemeh Bahrehmand Mitra Abbasifard |
| author_sort | Zahra Bagheri-Hosseinabadi |
| collection | DOAJ |
| description | Abstract Background Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their association with serum level of interleukin (IL)-1β in patients with coronavirus disease 2019 (COVID-19). Methods Thirty COVID-19 patients and 30 healthy subjects (HS) were recruited. Peripheral blood specimens were collected from subjects to assess NLRP1, NLRP3, and ASC gene expression by Real time-PCR technique. Serum levels of IL-1β were also measured via the enzyme-linked immunosorbent assay (ELISA). Results The findings showed no significant differences in serum IL-1β level between COVID-19 patients and the HS group. mRNA expression of ASC (P = 0.008) and NLRP1 (P = 0.03) gene had a significant increase in COVID-19 patients compared to HS, while there was no significant increase in the expression of NLRP3 between the studied group. There were significant correlations between patient’s data and expression levels of NLRP1, NLRP3, IL-1β, and ACS. Conclusions NLRP1 and ASC may have a more critical role in the generation of the active form of IL-1β in COVID-19 patients compared to NLRP3. However, serum levels of IL-1β in patients did not show a significant increase, which may be due to the patient’s condition and the application of virus escape mechanisms through impaired NLRP3 expression and its malfunction. |
| first_indexed | 2024-03-10T22:03:01Z |
| format | Article |
| id | doaj.art-17fdd7dd9248468288f45e8d184baca2 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-03-10T22:03:01Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-17fdd7dd9248468288f45e8d184baca22023-11-19T12:52:06ZengBMCBMC Immunology1471-21722023-09-0124111010.1186/s12865-023-00568-xEvaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19Zahra Bagheri-Hosseinabadi0Ali Shamsizadeh1Fatemeh Bahrehmand2Mitra Abbasifard3Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical SciencesPhysiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Ali-Ibn Abi-Talib Hospital, Rafsanjan University of Medical SciencesDepartment of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical SciencesImmunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical SciencesAbstract Background Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their association with serum level of interleukin (IL)-1β in patients with coronavirus disease 2019 (COVID-19). Methods Thirty COVID-19 patients and 30 healthy subjects (HS) were recruited. Peripheral blood specimens were collected from subjects to assess NLRP1, NLRP3, and ASC gene expression by Real time-PCR technique. Serum levels of IL-1β were also measured via the enzyme-linked immunosorbent assay (ELISA). Results The findings showed no significant differences in serum IL-1β level between COVID-19 patients and the HS group. mRNA expression of ASC (P = 0.008) and NLRP1 (P = 0.03) gene had a significant increase in COVID-19 patients compared to HS, while there was no significant increase in the expression of NLRP3 between the studied group. There were significant correlations between patient’s data and expression levels of NLRP1, NLRP3, IL-1β, and ACS. Conclusions NLRP1 and ASC may have a more critical role in the generation of the active form of IL-1β in COVID-19 patients compared to NLRP3. However, serum levels of IL-1β in patients did not show a significant increase, which may be due to the patient’s condition and the application of virus escape mechanisms through impaired NLRP3 expression and its malfunction.https://doi.org/10.1186/s12865-023-00568-xCoronavirus disease 2019Severe acute respiratory syndrome coronavirus 2InflammasomeIL-1βNLRP1NLRP3 |
| spellingShingle | Zahra Bagheri-Hosseinabadi Ali Shamsizadeh Fatemeh Bahrehmand Mitra Abbasifard Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19 BMC Immunology Coronavirus disease 2019 Severe acute respiratory syndrome coronavirus 2 Inflammasome IL-1β NLRP1 NLRP3 |
| title | Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19 |
| title_full | Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19 |
| title_fullStr | Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19 |
| title_full_unstemmed | Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19 |
| title_short | Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19 |
| title_sort | evaluation of the relationship between serum interleukin 1β levels and expression of inflammasome related genes in patients with covid 19 |
| topic | Coronavirus disease 2019 Severe acute respiratory syndrome coronavirus 2 Inflammasome IL-1β NLRP1 NLRP3 |
| url | https://doi.org/10.1186/s12865-023-00568-x |
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