Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica
As a result of more restrictive legislation regarding the use of pesticides, over the last two decades, the demand for new soil fumigants has increased. These compounds can come from a variety of sources, including organic residues. In this study, we identified in the cassava wastewater volatiloma a...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-09-01
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| Series: | Journal of Nematology |
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| Online Access: | https://doi.org/10.2478/jofnem-2022-0031 |
| _version_ | 1797405090730999808 |
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| author | Souza Simone Ribeiro de Campos Vicente Paulo Paula Letícia Lopes de Pedroso Marcio Pozzobon Terra Willian César |
| author_facet | Souza Simone Ribeiro de Campos Vicente Paulo Paula Letícia Lopes de Pedroso Marcio Pozzobon Terra Willian César |
| author_sort | Souza Simone Ribeiro de |
| collection | DOAJ |
| description | As a result of more restrictive legislation regarding the use of pesticides, over the last two decades, the demand for new soil fumigants has increased. These compounds can come from a variety of sources, including organic residues. In this study, we identified in the cassava wastewater volatiloma a molecule with potential to be developed as a new fumigant nematicide. Cassava wastewater (20 mL) releases volatile compounds toxic to Meloidogyne javanica second-stage juveniles (J2), causing J2 mortality up to 89%. Through gas chromatography–mass spectrometry, eight compounds were identified in the cassava wastewater volatiloma, with alcohols being the major class. The compounds ethyl butanoate and butyric acid identified in cassava wastewater volatiloma were selected for in vitro nematicidal activities and substrate fumigation tests. The lethal concentration predicted LC50–48 hr values (effective doses to kill 50% of M. javanica J2 population after 48 h of exposure) were 172.6 μg ∙ mL-1 and 301.2 μg ∙ mL-1 for butyric acid and ethyl butanoate, respectively. In a pot assay, the application of butyric acid and ethyl butanoate as a soil fumigant, at a dose of 0.5 mL ∙ kg-1 substrate, significantly (P < 0.05) decreased M. javanica infectivity and reproduction compared to the negative control (water). However, ethyl butanoate proved to be a more efficient soil fumigant (P < 0.05) than butyric acid, as its application reduced the number of galls and eggs to the level of the commercial fumigant Dazomet. This study is the first to demonstrate the potential of ethyl butanoate as a soil fumigant against M. javanica. |
| first_indexed | 2024-03-09T03:04:39Z |
| format | Article |
| id | doaj.art-17fdf57634894dbea157fe6054e97604 |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-03-09T03:04:39Z |
| publishDate | 2022-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-17fdf57634894dbea157fe6054e976042023-12-04T08:04:12ZengSciendoJournal of Nematology2640-396X2022-09-015416120612510.2478/jofnem-2022-0031Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanicaSouza Simone Ribeiro de0Campos Vicente Paulo1Paula Letícia Lopes de2Pedroso Marcio Pozzobon3Terra Willian César4Department of Plant Pathology, Universidade Federal de Lavras, Lavras, MG, BrazilDepartment of Plant Pathology, Universidade Federal de Lavras, Lavras, MG, BrazilDepartment of Plant Pathology, Universidade Federal de Lavras, Lavras, MG, BrazilDepartment of Chemistry, Universidade Federal de Lavras, Lavras, MG, BrazilDepartment of Plant Pathology, Universidade Federal de Lavras, Lavras, MG, BrazilAs a result of more restrictive legislation regarding the use of pesticides, over the last two decades, the demand for new soil fumigants has increased. These compounds can come from a variety of sources, including organic residues. In this study, we identified in the cassava wastewater volatiloma a molecule with potential to be developed as a new fumigant nematicide. Cassava wastewater (20 mL) releases volatile compounds toxic to Meloidogyne javanica second-stage juveniles (J2), causing J2 mortality up to 89%. Through gas chromatography–mass spectrometry, eight compounds were identified in the cassava wastewater volatiloma, with alcohols being the major class. The compounds ethyl butanoate and butyric acid identified in cassava wastewater volatiloma were selected for in vitro nematicidal activities and substrate fumigation tests. The lethal concentration predicted LC50–48 hr values (effective doses to kill 50% of M. javanica J2 population after 48 h of exposure) were 172.6 μg ∙ mL-1 and 301.2 μg ∙ mL-1 for butyric acid and ethyl butanoate, respectively. In a pot assay, the application of butyric acid and ethyl butanoate as a soil fumigant, at a dose of 0.5 mL ∙ kg-1 substrate, significantly (P < 0.05) decreased M. javanica infectivity and reproduction compared to the negative control (water). However, ethyl butanoate proved to be a more efficient soil fumigant (P < 0.05) than butyric acid, as its application reduced the number of galls and eggs to the level of the commercial fumigant Dazomet. This study is the first to demonstrate the potential of ethyl butanoate as a soil fumigant against M. javanica.https://doi.org/10.2478/jofnem-2022-0031meloidogynenematicidal activityorganic residuesvolatiles |
| spellingShingle | Souza Simone Ribeiro de Campos Vicente Paulo Paula Letícia Lopes de Pedroso Marcio Pozzobon Terra Willian César Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica Journal of Nematology meloidogyne nematicidal activity organic residues volatiles |
| title | Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica |
| title_full | Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica |
| title_fullStr | Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica |
| title_full_unstemmed | Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica |
| title_short | Ethyl Butanoate, Constituent of Cassava Wastewater Volatiloma, a Potential Soil Fumigant Against Meloidogyne javanica |
| title_sort | ethyl butanoate constituent of cassava wastewater volatiloma a potential soil fumigant against meloidogyne javanica |
| topic | meloidogyne nematicidal activity organic residues volatiles |
| url | https://doi.org/10.2478/jofnem-2022-0031 |
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