The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents
This paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding stra...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-03-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2306-5354/4/1/20 |
| _version_ | 1797707638704701440 |
|---|---|
| author | Liliana Montano-Herrera Bronwyn Laycock Alan Werker Steven Pratt |
| author_facet | Liliana Montano-Herrera Bronwyn Laycock Alan Werker Steven Pratt |
| author_sort | Liliana Montano-Herrera |
| collection | DOAJ |
| description | This paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated. |
| first_indexed | 2024-03-12T06:10:20Z |
| format | Article |
| id | doaj.art-17fe3c29b4244271a0fdc52e07d60818 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T06:10:20Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-17fe3c29b4244271a0fdc52e07d608182023-09-03T03:06:15ZengMDPI AGBioengineering2306-53542017-03-01412010.3390/bioengineering4010020bioengineering4010020The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing EquivalentsLiliana Montano-Herrera0Bronwyn Laycock1Alan Werker2Steven Pratt3School of Chemical Engineering, University of Queensland, St Lucia QLD 4072, AustraliaSchool of Chemical Engineering, University of Queensland, St Lucia QLD 4072, AustraliaVeolia Water Technologies AB—AnoxKaldnes, Klosterängsvägen 11A SE-226 47 Lund, SwedenSchool of Chemical Engineering, University of Queensland, St Lucia QLD 4072, AustraliaThis paper presents a systematic investigation into monomer development during mixed culture Polyhydroxyalkanoates (PHA) accumulation involving concurrent active biomass growth and polymer storage. A series of mixed culture PHA accumulation experiments, using several different substrate-feeding strategies, was carried out. The feedstock comprised volatile fatty acids, which were applied as single carbon sources, as mixtures, or in series, using a fed-batch feed-on-demand controlled bioprocess. A dynamic trend in active biomass growth as well as polymer composition was observed. The observations were consistent over replicate accumulations. Metabolic flux analysis (MFA) was used to investigate metabolic activity through time. It was concluded that carbon flux, and consequently copolymer composition, could be linked with how reducing equivalents are generated.http://www.mdpi.com/2306-5354/4/1/20PHAmonomer evolutionmixed culturemodelingpolymer compositionbiopolymer |
| spellingShingle | Liliana Montano-Herrera Bronwyn Laycock Alan Werker Steven Pratt The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents Bioengineering PHA monomer evolution mixed culture modeling polymer composition biopolymer |
| title | The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents |
| title_full | The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents |
| title_fullStr | The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents |
| title_full_unstemmed | The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents |
| title_short | The Evolution of Polymer Composition during PHA Accumulation: The Significance of Reducing Equivalents |
| title_sort | evolution of polymer composition during pha accumulation the significance of reducing equivalents |
| topic | PHA monomer evolution mixed culture modeling polymer composition biopolymer |
| url | http://www.mdpi.com/2306-5354/4/1/20 |
| work_keys_str_mv | AT lilianamontanoherrera theevolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT bronwynlaycock theevolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT alanwerker theevolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT stevenpratt theevolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT lilianamontanoherrera evolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT bronwynlaycock evolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT alanwerker evolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents AT stevenpratt evolutionofpolymercompositionduringphaaccumulationthesignificanceofreducingequivalents |