Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma
Cancer-associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p-EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single-cell RNA sequencing datasets...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6470 |
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| author | Nam Cong-Nhat Huynh Tze-Ta Huang Chi Thi-Kim Nguyen Fang-Kuei Lin |
| author_facet | Nam Cong-Nhat Huynh Tze-Ta Huang Chi Thi-Kim Nguyen Fang-Kuei Lin |
| author_sort | Nam Cong-Nhat Huynh |
| collection | DOAJ |
| description | Cancer-associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p-EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single-cell RNA sequencing datasets to define OSCC CAFs and p-EMT subpopulations. We highlighted the cell–cell interaction network of CAFs and p-EMT tumor cells and suggested biomarkers for the diagnosis and prognosis of OSCC during the metastasis condition. The analysis discovered four subtypes of CAFs: one p-EMT tumor cell population, and cycling tumor cells as well as TNFSF12-TNFRSF25/TNFRSF12A interactions between CAFs and p-EMT tumor cells during tumor metastasis. This suggests the prediction of therapeutically targetable checkpoint receptor–ligand interactions between CAFs and p-EMT tumor cells in OSCC regarding the metastasis status. |
| first_indexed | 2024-03-09T23:35:43Z |
| format | Article |
| id | doaj.art-1800acea854645c18a5ae78a5502466d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:35:43Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1800acea854645c18a5ae78a5502466d2023-11-23T17:01:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312647010.3390/ijms23126470Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell CarcinomaNam Cong-Nhat Huynh0Tze-Ta Huang1Chi Thi-Kim Nguyen2Fang-Kuei Lin3Laboratory of Oral-Maxillofacial Biology, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 749000, VietnamInstitute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701401, TaiwanDepartment of Oral Pathology, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 749000, VietnamInstitute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan 701401, TaiwanCancer-associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p-EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single-cell RNA sequencing datasets to define OSCC CAFs and p-EMT subpopulations. We highlighted the cell–cell interaction network of CAFs and p-EMT tumor cells and suggested biomarkers for the diagnosis and prognosis of OSCC during the metastasis condition. The analysis discovered four subtypes of CAFs: one p-EMT tumor cell population, and cycling tumor cells as well as TNFSF12-TNFRSF25/TNFRSF12A interactions between CAFs and p-EMT tumor cells during tumor metastasis. This suggests the prediction of therapeutically targetable checkpoint receptor–ligand interactions between CAFs and p-EMT tumor cells in OSCC regarding the metastasis status.https://www.mdpi.com/1422-0067/23/12/6470single-cell RNA sequencingoral squamous cell carcinomacancer-associated fibroblastspartial epithelial–mesenchymal transitionmetastasis |
| spellingShingle | Nam Cong-Nhat Huynh Tze-Ta Huang Chi Thi-Kim Nguyen Fang-Kuei Lin Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma International Journal of Molecular Sciences single-cell RNA sequencing oral squamous cell carcinoma cancer-associated fibroblasts partial epithelial–mesenchymal transition metastasis |
| title | Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma |
| title_full | Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma |
| title_fullStr | Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma |
| title_short | Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma |
| title_sort | comprehensive integrated single cell whole transcriptome analysis revealed the p emt tumor cells cafs communication in oral squamous cell carcinoma |
| topic | single-cell RNA sequencing oral squamous cell carcinoma cancer-associated fibroblasts partial epithelial–mesenchymal transition metastasis |
| url | https://www.mdpi.com/1422-0067/23/12/6470 |
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