Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey.
Bacillus cereus is the 2nd most frequent bacterial agent responsible for food-borne outbreaks in France and the 3rd in Europe. In addition, local and systemic infections have been reported, mainly describing individual cases or single hospital setting. The real incidence of such infection is unknown...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5966241?pdf=render |
| _version_ | 1818324738755264512 |
|---|---|
| author | Benjamin Glasset Sabine Herbin Sophie A Granier Laurent Cavalié Emilie Lafeuille Cyprien Guérin Raymond Ruimy Florence Casagrande-Magne Marion Levast Nathalie Chautemps Jean-Winoc Decousser Laure Belotti Isabelle Pelloux Jerôme Robert Anne Brisabois Nalini Ramarao |
| author_facet | Benjamin Glasset Sabine Herbin Sophie A Granier Laurent Cavalié Emilie Lafeuille Cyprien Guérin Raymond Ruimy Florence Casagrande-Magne Marion Levast Nathalie Chautemps Jean-Winoc Decousser Laure Belotti Isabelle Pelloux Jerôme Robert Anne Brisabois Nalini Ramarao |
| author_sort | Benjamin Glasset |
| collection | DOAJ |
| description | Bacillus cereus is the 2nd most frequent bacterial agent responsible for food-borne outbreaks in France and the 3rd in Europe. In addition, local and systemic infections have been reported, mainly describing individual cases or single hospital setting. The real incidence of such infection is unknown and information on genetic and phenotypic characteristics of the incriminated strains is generally scarce. We performed an extensive study of B. cereus strains isolated from patients and hospital environments from nine hospitals during a 5-year study, giving an overview of the consequences, sources and pathogenic patterns of B. cereus clinical infections. We demonstrated the occurrence of several hospital-cross-contaminations. Identical B. cereus strains were recovered from different patients and hospital environments for up to 2 years. We also clearly revealed the occurrence of inter hospital contaminations by the same strain. These cases represent the first documented events of nosocomial epidemy by B. cereus responsible for intra and inter hospitals contaminations. Indeed, contamination of different patients with the same strain of B. cereus was so far never shown. In addition, we propose a scheme for the characterization of B. cereus based on biochemical properties and genetic identification and highlight that main genetic signatures may carry a high pathogenic potential. Moreover, the characterization of antibiotic resistance shows an acquired resistance phenotype for rifampicin. This may provide indication to adjust the antibiotic treatment and care of patients. |
| first_indexed | 2024-12-13T11:33:22Z |
| format | Article |
| id | doaj.art-1802454cec0649d58440b2279d40352d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T11:33:22Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1802454cec0649d58440b2279d40352d2022-12-21T23:47:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01135e019434610.1371/journal.pone.0194346Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey.Benjamin GlassetSabine HerbinSophie A GranierLaurent CavaliéEmilie LafeuilleCyprien GuérinRaymond RuimyFlorence Casagrande-MagneMarion LevastNathalie ChautempsJean-Winoc DecousserLaure BelottiIsabelle PellouxJerôme RobertAnne BrisaboisNalini RamaraoBacillus cereus is the 2nd most frequent bacterial agent responsible for food-borne outbreaks in France and the 3rd in Europe. In addition, local and systemic infections have been reported, mainly describing individual cases or single hospital setting. The real incidence of such infection is unknown and information on genetic and phenotypic characteristics of the incriminated strains is generally scarce. We performed an extensive study of B. cereus strains isolated from patients and hospital environments from nine hospitals during a 5-year study, giving an overview of the consequences, sources and pathogenic patterns of B. cereus clinical infections. We demonstrated the occurrence of several hospital-cross-contaminations. Identical B. cereus strains were recovered from different patients and hospital environments for up to 2 years. We also clearly revealed the occurrence of inter hospital contaminations by the same strain. These cases represent the first documented events of nosocomial epidemy by B. cereus responsible for intra and inter hospitals contaminations. Indeed, contamination of different patients with the same strain of B. cereus was so far never shown. In addition, we propose a scheme for the characterization of B. cereus based on biochemical properties and genetic identification and highlight that main genetic signatures may carry a high pathogenic potential. Moreover, the characterization of antibiotic resistance shows an acquired resistance phenotype for rifampicin. This may provide indication to adjust the antibiotic treatment and care of patients.http://europepmc.org/articles/PMC5966241?pdf=render |
| spellingShingle | Benjamin Glasset Sabine Herbin Sophie A Granier Laurent Cavalié Emilie Lafeuille Cyprien Guérin Raymond Ruimy Florence Casagrande-Magne Marion Levast Nathalie Chautemps Jean-Winoc Decousser Laure Belotti Isabelle Pelloux Jerôme Robert Anne Brisabois Nalini Ramarao Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey. PLoS ONE |
| title | Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey. |
| title_full | Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey. |
| title_fullStr | Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey. |
| title_full_unstemmed | Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey. |
| title_short | Bacillus cereus, a serious cause of nosocomial infections: Epidemiologic and genetic survey. |
| title_sort | bacillus cereus a serious cause of nosocomial infections epidemiologic and genetic survey |
| url | http://europepmc.org/articles/PMC5966241?pdf=render |
| work_keys_str_mv | AT benjaminglasset bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT sabineherbin bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT sophieagranier bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT laurentcavalie bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT emilielafeuille bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT cyprienguerin bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT raymondruimy bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT florencecasagrandemagne bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT marionlevast bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT nathaliechautemps bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT jeanwinocdecousser bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT laurebelotti bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT isabellepelloux bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT jeromerobert bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT annebrisabois bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey AT naliniramarao bacilluscereusaseriouscauseofnosocomialinfectionsepidemiologicandgeneticsurvey |