Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera
Herein, we report the synthesis and functionalization of gadolinium oxide nanoparticles (Gd<sub>2</sub>O<sub>3</sub> NPs) to fabricate a highly efficient immunosensor for the detection of <i>Vibrio cholera</i> toxin (CT). Gd<sub>2</sub>O<sub>3<...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/177 |
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| author | Ashutosh Kumar Tamal Sarkar Pratima R. Solanki |
| author_facet | Ashutosh Kumar Tamal Sarkar Pratima R. Solanki |
| author_sort | Ashutosh Kumar |
| collection | DOAJ |
| description | Herein, we report the synthesis and functionalization of gadolinium oxide nanoparticles (Gd<sub>2</sub>O<sub>3</sub> NPs) to fabricate a highly efficient immunosensor for the detection of <i>Vibrio cholera</i> toxin (CT). Gd<sub>2</sub>O<sub>3</sub> NPs were produced in a straightforward manner utilizing the microwave irradiation technique using a domestic microwave oven. X-ray diffraction, transmission electron microscopy, and spectroscopic techniques were used to characterize the structural and physical aspects of Gd<sub>2</sub>O<sub>3</sub> NPs. The Gd<sub>2</sub>O<sub>3</sub> NPs were then functionalized with 3-(Aminopropyl) triethoxysilane (APTES) and electrophoretically deposited onto an ITO-coated glass substrate. The anti-CT monoclonal antibodies were covalently attached to the APTES-Gd<sub>2</sub>O<sub>3</sub>/ITO electrode via EDC-NHS chemistry, followed by bovine serum albumin (BSA). For CT detection, electrochemical response experiments using BSA/anti-CT/APTES-Gd<sub>2</sub>O<sub>3</sub>/ITO immunoelectrodes were carried out (5–700 ng mL<sup>−1</sup>). The immunoelectrode demonstrated an outstanding electrochemical reaction against CT, with a sensitivity of 8.37 mA ng<sup>−1</sup> mL cm<sup>−2</sup> and a detection limit of 1.48 ng mL<sup>−1</sup>. |
| first_indexed | 2024-03-11T09:04:42Z |
| format | Article |
| id | doaj.art-1803d5531c8e43f4ac16b2138a72be7b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:04:42Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1803d5531c8e43f4ac16b2138a72be7b2023-11-16T19:25:07ZengMDPI AGBiosensors2079-63742023-01-0113217710.3390/bios13020177Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for CholeraAshutosh Kumar0Tamal Sarkar1Pratima R. Solanki2Department of Electrical Engineering, University of Notre Dame, Notre Dame, IN 46637, USADepartment of Physics, Indian Institute of Technology Kharagpur, Kharagpur 721302, IndiaSpecial Centre for Nanoscience, Jawaharlal Nehru University, New Delhi 110067, IndiaHerein, we report the synthesis and functionalization of gadolinium oxide nanoparticles (Gd<sub>2</sub>O<sub>3</sub> NPs) to fabricate a highly efficient immunosensor for the detection of <i>Vibrio cholera</i> toxin (CT). Gd<sub>2</sub>O<sub>3</sub> NPs were produced in a straightforward manner utilizing the microwave irradiation technique using a domestic microwave oven. X-ray diffraction, transmission electron microscopy, and spectroscopic techniques were used to characterize the structural and physical aspects of Gd<sub>2</sub>O<sub>3</sub> NPs. The Gd<sub>2</sub>O<sub>3</sub> NPs were then functionalized with 3-(Aminopropyl) triethoxysilane (APTES) and electrophoretically deposited onto an ITO-coated glass substrate. The anti-CT monoclonal antibodies were covalently attached to the APTES-Gd<sub>2</sub>O<sub>3</sub>/ITO electrode via EDC-NHS chemistry, followed by bovine serum albumin (BSA). For CT detection, electrochemical response experiments using BSA/anti-CT/APTES-Gd<sub>2</sub>O<sub>3</sub>/ITO immunoelectrodes were carried out (5–700 ng mL<sup>−1</sup>). The immunoelectrode demonstrated an outstanding electrochemical reaction against CT, with a sensitivity of 8.37 mA ng<sup>−1</sup> mL cm<sup>−2</sup> and a detection limit of 1.48 ng mL<sup>−1</sup>.https://www.mdpi.com/2079-6374/13/2/177gadolinium oxide nanoparticlesAPTESimmunosensor<i>Vibrio cholerae</i> |
| spellingShingle | Ashutosh Kumar Tamal Sarkar Pratima R. Solanki Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera Biosensors gadolinium oxide nanoparticles APTES immunosensor <i>Vibrio cholerae</i> |
| title | Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera |
| title_full | Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera |
| title_fullStr | Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera |
| title_full_unstemmed | Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera |
| title_short | Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera |
| title_sort | amine functionalized gadolinium oxide nanoparticles based electrochemical immunosensor for cholera |
| topic | gadolinium oxide nanoparticles APTES immunosensor <i>Vibrio cholerae</i> |
| url | https://www.mdpi.com/2079-6374/13/2/177 |
| work_keys_str_mv | AT ashutoshkumar aminefunctionalizedgadoliniumoxidenanoparticlesbasedelectrochemicalimmunosensorforcholera AT tamalsarkar aminefunctionalizedgadoliniumoxidenanoparticlesbasedelectrochemicalimmunosensorforcholera AT pratimarsolanki aminefunctionalizedgadoliniumoxidenanoparticlesbasedelectrochemicalimmunosensorforcholera |