Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically o...
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| Format: | Article |
| Language: | English |
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Elsevier España
2011-09-01
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| Series: | Revista Argentina de Microbiología |
| Subjects: | |
| Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412011000300010 |
| _version_ | 1818318052980162560 |
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| author | Miguel A Galvagno Leopoldo J Iannone Jorgelina Bianchi Florencia Kronberg Enrique Rost Maria R Carstens Patricia Cerrutti |
| author_facet | Miguel A Galvagno Leopoldo J Iannone Jorgelina Bianchi Florencia Kronberg Enrique Rost Maria R Carstens Patricia Cerrutti |
| author_sort | Miguel A Galvagno |
| collection | DOAJ |
| description | The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically optimized by Response Surface Methodology in media containing biodiesel-derived glycerol as main carbon source. This strain exhibited distinctive morphological and fatty acid profile characteristics, and showed an increased extracellular lipase activity. An organic source of nitrogen and the addition of 1.0 g/l olive oil were necessary for significant lipase production. Plackett-Burman and Central Composite Statistical Designs were employed for screening and optimization of fermentation in shaken flasks cultures, and the maximum values obtained were 16.1 g/l for biomass and 12.2 Units/ml for lipase, respectively. Optimized batch bioprocess was thereafter scaled in aerated bioreactors and the values reached for lipase specific activity after 95 % of the glycerol had been consumed, were three-fold higher than those obtained in shaken flasks cultures. A sustainable bioprocess to obtain biomass and extracellular lipase activity was attained by maximizing the use of the by-products of biodiesel industry.<br>Optimización de la producción de biomasa usando glicerol crudo, de una cepa mutante de Yarrowia lipolytica con actividad incrementada de lipasa. La levadura Yarrowia lipolytica acumula aceites y produce una lipasa extracelular al crecer en diferentes fuentes de carbono, entre ellas el glicerol, principal subproducto de la creciente industria del biodiésel. En el presente trabajo, se optimizó mediante la metodología de superficies de respuesta la producción de biomasa de una nueva cepa mutante de Y. lipolytica, empleando medios con glicerol derivado de la industria del biodiésel como principal fuente de carbono. Esta cepa presentó características morfológicas y perfil de ácidos grasos distintivos, y una mayor actividad de lipasa extracelular. Para obtener una producción significativa de lipasa extracelular, fue necesario el agregado de una fuente orgánica de nitrógeno y de 1 g/l de aceite de oliva. Se utilizaron los diseños estadísticos de Plackett-Burman y central compuesto para la selección y la optimización de las fermentaciones en frascos agitados; los máximos valores de biomasa y de lipasa obtenidos fueron de 16,1 g/l y 12,2 unidades/ml, respectivamente. Luego, el bioproceso en lote optimizado se escaló a biorreactores aireados, y los valores de actividad específica de lipasa alcanzados después de haberse consumido el 95 % del glicerol fueron tres veces más altos que los obtenidos en los cultivos en frascos agitados. En suma, se desarrolló un bioproceso sostenible para la obtención de biomasa y de una actividad de lipasa extracelular, que a la vez maximiza el uso de subproductos de la industria del biodiésel. |
| first_indexed | 2024-12-13T09:47:06Z |
| format | Article |
| id | doaj.art-18050e5ac6324870ade1ca8a455ad791 |
| institution | Directory Open Access Journal |
| issn | 0325-7541 1851-7617 |
| language | English |
| last_indexed | 2024-12-13T09:47:06Z |
| publishDate | 2011-09-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Revista Argentina de Microbiología |
| spelling | doaj.art-18050e5ac6324870ade1ca8a455ad7912022-12-21T23:52:01ZengElsevier EspañaRevista Argentina de Microbiología0325-75411851-76172011-09-01433218225Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerolMiguel A GalvagnoLeopoldo J IannoneJorgelina BianchiFlorencia KronbergEnrique RostMaria R CarstensPatricia CerruttiThe yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically optimized by Response Surface Methodology in media containing biodiesel-derived glycerol as main carbon source. This strain exhibited distinctive morphological and fatty acid profile characteristics, and showed an increased extracellular lipase activity. An organic source of nitrogen and the addition of 1.0 g/l olive oil were necessary for significant lipase production. Plackett-Burman and Central Composite Statistical Designs were employed for screening and optimization of fermentation in shaken flasks cultures, and the maximum values obtained were 16.1 g/l for biomass and 12.2 Units/ml for lipase, respectively. Optimized batch bioprocess was thereafter scaled in aerated bioreactors and the values reached for lipase specific activity after 95 % of the glycerol had been consumed, were three-fold higher than those obtained in shaken flasks cultures. A sustainable bioprocess to obtain biomass and extracellular lipase activity was attained by maximizing the use of the by-products of biodiesel industry.<br>Optimización de la producción de biomasa usando glicerol crudo, de una cepa mutante de Yarrowia lipolytica con actividad incrementada de lipasa. La levadura Yarrowia lipolytica acumula aceites y produce una lipasa extracelular al crecer en diferentes fuentes de carbono, entre ellas el glicerol, principal subproducto de la creciente industria del biodiésel. En el presente trabajo, se optimizó mediante la metodología de superficies de respuesta la producción de biomasa de una nueva cepa mutante de Y. lipolytica, empleando medios con glicerol derivado de la industria del biodiésel como principal fuente de carbono. Esta cepa presentó características morfológicas y perfil de ácidos grasos distintivos, y una mayor actividad de lipasa extracelular. Para obtener una producción significativa de lipasa extracelular, fue necesario el agregado de una fuente orgánica de nitrógeno y de 1 g/l de aceite de oliva. Se utilizaron los diseños estadísticos de Plackett-Burman y central compuesto para la selección y la optimización de las fermentaciones en frascos agitados; los máximos valores de biomasa y de lipasa obtenidos fueron de 16,1 g/l y 12,2 unidades/ml, respectivamente. Luego, el bioproceso en lote optimizado se escaló a biorreactores aireados, y los valores de actividad específica de lipasa alcanzados después de haberse consumido el 95 % del glicerol fueron tres veces más altos que los obtenidos en los cultivos en frascos agitados. En suma, se desarrolló un bioproceso sostenible para la obtención de biomasa y de una actividad de lipasa extracelular, que a la vez maximiza el uso de subproductos de la industria del biodiésel.http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412011000300010Mutante de Yarrowia lipolyticaBiomasaLipasaÁcidos grasosGlicerolYarrowia lipolytica mutantBiomassLipaseFatty acidsGlycerol |
| spellingShingle | Miguel A Galvagno Leopoldo J Iannone Jorgelina Bianchi Florencia Kronberg Enrique Rost Maria R Carstens Patricia Cerrutti Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol Revista Argentina de Microbiología Mutante de Yarrowia lipolytica Biomasa Lipasa Ácidos grasos Glicerol Yarrowia lipolytica mutant Biomass Lipase Fatty acids Glycerol |
| title | Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol |
| title_full | Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol |
| title_fullStr | Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol |
| title_full_unstemmed | Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol |
| title_short | Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol |
| title_sort | optimization of biomass production of a mutant of yarrowia lipolytica with an increased lipase activity using raw glycerol |
| topic | Mutante de Yarrowia lipolytica Biomasa Lipasa Ácidos grasos Glicerol Yarrowia lipolytica mutant Biomass Lipase Fatty acids Glycerol |
| url | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412011000300010 |
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