Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens
Sm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that com...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1997-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500014 |
| _version_ | 1797703088762519552 |
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| author | Frederico GC Abath Edeneide M Xavier Joanne D'Arc B Silva Marcos A Morais Junior Silvia ML Montenegro |
| author_facet | Frederico GC Abath Edeneide M Xavier Joanne D'Arc B Silva Marcos A Morais Junior Silvia ML Montenegro |
| author_sort | Frederico GC Abath |
| collection | DOAJ |
| description | Sm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that comparisons could be made among different expression systems and different genes. The fusion proteins corresponding to several parts of the gene for the precursor of Sm15 failed in producing antibodies recognizing the parasite counterpart. On the other hand, antibodies raised against Sm13 MBP-fusion proteins recognized the 13 kDa tegumental protein. Thus the peculiarities of the gene of interest are important and the choice of the expression system must sometimes be decided on an empirical basis |
| first_indexed | 2024-03-12T04:59:22Z |
| format | Article |
| id | doaj.art-1805843f7f694cb999923e8beb28b15d |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:59:22Z |
| publishDate | 1997-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1805843f7f694cb999923e8beb28b15d2023-09-03T09:14:14ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601997-09-0192563710.1590/S0074-02761997000500014Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental AntigensFrederico GC AbathEdeneide M XavierJoanne D'Arc B SilvaMarcos A Morais JuniorSilvia ML MontenegroSm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that comparisons could be made among different expression systems and different genes. The fusion proteins corresponding to several parts of the gene for the precursor of Sm15 failed in producing antibodies recognizing the parasite counterpart. On the other hand, antibodies raised against Sm13 MBP-fusion proteins recognized the 13 kDa tegumental protein. Thus the peculiarities of the gene of interest are important and the choice of the expression system must sometimes be decided on an empirical basishttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500014Schistosoma mansonitegumental antigensfusion proteins |
| spellingShingle | Frederico GC Abath Edeneide M Xavier Joanne D'Arc B Silva Marcos A Morais Junior Silvia ML Montenegro Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens Memorias do Instituto Oswaldo Cruz Schistosoma mansoni tegumental antigens fusion proteins |
| title | Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens |
| title_full | Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens |
| title_fullStr | Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens |
| title_full_unstemmed | Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens |
| title_short | Expression of Recombinant Antigens in Escherichia coli: Application on Immunochemical Studies of Schistosoma mansoni Tegumental Antigens |
| title_sort | expression of recombinant antigens in escherichia coli application on immunochemical studies of schistosoma mansoni tegumental antigens |
| topic | Schistosoma mansoni tegumental antigens fusion proteins |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500014 |
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