Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene
Abstract Crosstalk between the gut microbiota and intestinal epithelium shapes the gut environment and profoundly influences the intestinal immune homeostasis. Glycosylphosphatidylinositol anchored proteins (GPI – APs) contribute to a variety of gut-associated immune functions, including microbial s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15150-5 |
| _version_ | 1818256949336080384 |
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| author | Aditi Jangid Shinji Fukuda Masahide Seki Yutaka Suzuki Todd D. Taylor Hiroshi Ohno Tulika Prakash |
| author_facet | Aditi Jangid Shinji Fukuda Masahide Seki Yutaka Suzuki Todd D. Taylor Hiroshi Ohno Tulika Prakash |
| author_sort | Aditi Jangid |
| collection | DOAJ |
| description | Abstract Crosstalk between the gut microbiota and intestinal epithelium shapes the gut environment and profoundly influences the intestinal immune homeostasis. Glycosylphosphatidylinositol anchored proteins (GPI – APs) contribute to a variety of gut-associated immune functions, including microbial surveillance and defense, and epithelial cell polarity. Properly polarised epithelial cells are essential for the establishment of the barrier function of gut epithelia. The Piga gene is one among seven genes that encode for an enzyme which is involved in the first step of GPI-anchor biosynthesis. This is the first study reporting a knockout of the intestinal epithelial cell-specific Piga gene (Piga-/-) and its association with the gut microbiota in mice using a whole metagenome shotgun-based sequencing approach. An overall reduced microbiota diversity has been observed in the Piga-/- group as compared to the control group (ANOVA p = 0.34). The taxonomic biomarkers, namely: Gammaproteobacteria (class), Enterobacterales (order), Enterobacteriaceae (family), Escherichia (genus), Proteus (genus) and Escherichia coli (species), increased more in the Piga-/- mice as compared to in the control group. Further, the pathogenic E. coli strains, namely E. coli O157:H7 str. EDL 933 (EHEC), E. coli CFT073 (UPEC) and E. coli 536 (UPEC), were found in the Piga-/- mice which also harbored virulence factor transporters. In addition, the taxa responsible for short chain fatty acid production were decreased in the Piga-/- group. The Piga-/- mice gut harbored an increased number of microbial functions responsible for the survival of pathogens in the inflamed gut environment. Our observations clearly indicate that the Piga-/- mice gut might have an overall enhancement in pathogenic behaviour and reduced capabilities beneficial to health. |
| first_indexed | 2024-12-12T17:35:53Z |
| format | Article |
| id | doaj.art-1806b405d24249e185752cd3765296a7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T17:35:53Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1806b405d24249e185752cd3765296a72022-12-22T00:17:12ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-15150-5Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga geneAditi Jangid0Shinji Fukuda1Masahide Seki2Yutaka Suzuki3Todd D. Taylor4Hiroshi Ohno5Tulika Prakash6BioX Centre and School of Basic Sciences, Indian Institute of Technology MandiInstitute for Advanced Biosciences, Keio UniversityDepartment of Computational Biology and Medical Sciences, The University of TokyoDepartment of Computational Biology and Medical Sciences, The University of TokyoLaboratory for Microbiome Sciences, RIKEN Center for Integrative Medical SciencesLaboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical SciencesBioX Centre and School of Basic Sciences, Indian Institute of Technology MandiAbstract Crosstalk between the gut microbiota and intestinal epithelium shapes the gut environment and profoundly influences the intestinal immune homeostasis. Glycosylphosphatidylinositol anchored proteins (GPI – APs) contribute to a variety of gut-associated immune functions, including microbial surveillance and defense, and epithelial cell polarity. Properly polarised epithelial cells are essential for the establishment of the barrier function of gut epithelia. The Piga gene is one among seven genes that encode for an enzyme which is involved in the first step of GPI-anchor biosynthesis. This is the first study reporting a knockout of the intestinal epithelial cell-specific Piga gene (Piga-/-) and its association with the gut microbiota in mice using a whole metagenome shotgun-based sequencing approach. An overall reduced microbiota diversity has been observed in the Piga-/- group as compared to the control group (ANOVA p = 0.34). The taxonomic biomarkers, namely: Gammaproteobacteria (class), Enterobacterales (order), Enterobacteriaceae (family), Escherichia (genus), Proteus (genus) and Escherichia coli (species), increased more in the Piga-/- mice as compared to in the control group. Further, the pathogenic E. coli strains, namely E. coli O157:H7 str. EDL 933 (EHEC), E. coli CFT073 (UPEC) and E. coli 536 (UPEC), were found in the Piga-/- mice which also harbored virulence factor transporters. In addition, the taxa responsible for short chain fatty acid production were decreased in the Piga-/- group. The Piga-/- mice gut harbored an increased number of microbial functions responsible for the survival of pathogens in the inflamed gut environment. Our observations clearly indicate that the Piga-/- mice gut might have an overall enhancement in pathogenic behaviour and reduced capabilities beneficial to health.https://doi.org/10.1038/s41598-022-15150-5 |
| spellingShingle | Aditi Jangid Shinji Fukuda Masahide Seki Yutaka Suzuki Todd D. Taylor Hiroshi Ohno Tulika Prakash Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene Scientific Reports |
| title | Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene |
| title_full | Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene |
| title_fullStr | Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene |
| title_full_unstemmed | Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene |
| title_short | Gut microbiota alternation under the intestinal epithelium-specific knockout of mouse Piga gene |
| title_sort | gut microbiota alternation under the intestinal epithelium specific knockout of mouse piga gene |
| url | https://doi.org/10.1038/s41598-022-15150-5 |
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