Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc
Abstract Background Disc degeneration is associated with repetitive violent injuries. This study aims to explore the impact of repetitive strikes loading on the biology and biomechanics of intervertebral discs (IVDs) using an organ culture model. Methods IVDs from the bovine tail were isolated and c...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-03-01
|
| Series: | JOR Spine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsp2.1314 |
| _version_ | 1797244248798527488 |
|---|---|
| author | Jiaxiang Zhou Jianmin Wang Jianfeng Li Zhengya Zhu Zhongyuan He Junhong Li Tao Tang Hongkun Chen Yukun Du Zhen Li Manman Gao Zhiyu Zhou Yongming Xi |
| author_facet | Jiaxiang Zhou Jianmin Wang Jianfeng Li Zhengya Zhu Zhongyuan He Junhong Li Tao Tang Hongkun Chen Yukun Du Zhen Li Manman Gao Zhiyu Zhou Yongming Xi |
| author_sort | Jiaxiang Zhou |
| collection | DOAJ |
| description | Abstract Background Disc degeneration is associated with repetitive violent injuries. This study aims to explore the impact of repetitive strikes loading on the biology and biomechanics of intervertebral discs (IVDs) using an organ culture model. Methods IVDs from the bovine tail were isolated and cultured in a bioreactor, with exposure to various loading conditions. The control group was subjected to physiological loading, while the model group was exposed to either one strike loading (compression at 38% of IVD height) or repetitive one strike loading (compression at 38% of IVD height). Disc height and dynamic compressive stiffness were measured after overnight swelling and loading. Furthermore, histological morphology, cell viability, and gene expression were analyzed on Day 32. Glycosaminoglycan (GAG) and nitric oxide (NO) release in conditioned medium were also analyzed. Results The repetitive one strike group exhibited early disc degeneration, characterized by decreased dynamic compression stiffness, the presence of annulus fibrosus clefts, and degradation of the extracellular matrix. Additionally, this group demonstrated significantly higher levels of cell death (p < 0.05) and glycosaminoglycan (GAG) release (p < 0.05) compared to the control group. Furthermore, upregulation of MMP1, MMP13, and ADAMTS5 was observed in both nucleus pulposus (NP) and annulus fibrosus (AF) tissues of the repetitive one strike group (p < 0.05). The one strike group exhibited annulus fibrosus clefts but showed no gene expression changes compared to the control group. Conclusions This study shows that repetitive violent injuries lead to the degeneration of a healthy bovine IVDs, thereby providing new insights into early‐stage disc degeneration. |
| first_indexed | 2024-04-24T19:08:00Z |
| format | Article |
| id | doaj.art-180ab81932d1406e809f327e895488a2 |
| institution | Directory Open Access Journal |
| issn | 2572-1143 |
| language | English |
| last_indexed | 2024-04-24T19:08:00Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj.art-180ab81932d1406e809f327e895488a22024-03-26T14:08:39ZengWileyJOR Spine2572-11432024-03-0171n/an/a10.1002/jsp2.1314Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral discJiaxiang Zhou0Jianmin Wang1Jianfeng Li2Zhengya Zhu3Zhongyuan He4Junhong Li5Tao Tang6Hongkun Chen7Yukun Du8Zhen Li9Manman Gao10Zhiyu Zhou11Yongming Xi12Department of Spinal Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaDepartment of Spinal Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaAO Research Institute Davos Davos SwitzerlandInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaInnovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen ChinaDepartment of Spinal Surgery The Affiliated Hospital of Qingdao University Qingdao ChinaAbstract Background Disc degeneration is associated with repetitive violent injuries. This study aims to explore the impact of repetitive strikes loading on the biology and biomechanics of intervertebral discs (IVDs) using an organ culture model. Methods IVDs from the bovine tail were isolated and cultured in a bioreactor, with exposure to various loading conditions. The control group was subjected to physiological loading, while the model group was exposed to either one strike loading (compression at 38% of IVD height) or repetitive one strike loading (compression at 38% of IVD height). Disc height and dynamic compressive stiffness were measured after overnight swelling and loading. Furthermore, histological morphology, cell viability, and gene expression were analyzed on Day 32. Glycosaminoglycan (GAG) and nitric oxide (NO) release in conditioned medium were also analyzed. Results The repetitive one strike group exhibited early disc degeneration, characterized by decreased dynamic compression stiffness, the presence of annulus fibrosus clefts, and degradation of the extracellular matrix. Additionally, this group demonstrated significantly higher levels of cell death (p < 0.05) and glycosaminoglycan (GAG) release (p < 0.05) compared to the control group. Furthermore, upregulation of MMP1, MMP13, and ADAMTS5 was observed in both nucleus pulposus (NP) and annulus fibrosus (AF) tissues of the repetitive one strike group (p < 0.05). The one strike group exhibited annulus fibrosus clefts but showed no gene expression changes compared to the control group. Conclusions This study shows that repetitive violent injuries lead to the degeneration of a healthy bovine IVDs, thereby providing new insights into early‐stage disc degeneration.https://doi.org/10.1002/jsp2.1314degenerationintervertebral discmechanical loadorgan culturerepetitive injury |
| spellingShingle | Jiaxiang Zhou Jianmin Wang Jianfeng Li Zhengya Zhu Zhongyuan He Junhong Li Tao Tang Hongkun Chen Yukun Du Zhen Li Manman Gao Zhiyu Zhou Yongming Xi Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc JOR Spine degeneration intervertebral disc mechanical load organ culture repetitive injury |
| title | Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc |
| title_full | Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc |
| title_fullStr | Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc |
| title_full_unstemmed | Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc |
| title_short | Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc |
| title_sort | repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc |
| topic | degeneration intervertebral disc mechanical load organ culture repetitive injury |
| url | https://doi.org/10.1002/jsp2.1314 |
| work_keys_str_mv | AT jiaxiangzhou repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT jianminwang repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT jianfengli repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT zhengyazhu repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT zhongyuanhe repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT junhongli repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT taotang repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT hongkunchen repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT yukundu repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT zhenli repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT manmangao repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT zhiyuzhou repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc AT yongmingxi repetitivestrikesloadingorganculturemodeltoinvestigatethebiologicalandbiomechanicalresponsesoftheintervertebraldisc |