Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.)
Strawberry tree fruits (<i>Arbutus unedo</i> L.) are a natural source of valuable biologically active compounds. Therefore, the aim of this work was to develop a green extraction approach using pressurized liquid extraction (PLE) to provide the highest yield of bioactive compounds. Diffe...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/10/2006 |
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| author | Anica Bebek Markovinović Sanja Milošević Nemanja Teslić Branimir Pavlić Predrag Putnik Irena Brčić Karačonji Karlo Jurica Dario Lasić Danijela Bursać Kovačević |
| author_facet | Anica Bebek Markovinović Sanja Milošević Nemanja Teslić Branimir Pavlić Predrag Putnik Irena Brčić Karačonji Karlo Jurica Dario Lasić Danijela Bursać Kovačević |
| author_sort | Anica Bebek Markovinović |
| collection | DOAJ |
| description | Strawberry tree fruits (<i>Arbutus unedo</i> L.) are a natural source of valuable biologically active compounds. Therefore, the aim of this work was to develop a green extraction approach using pressurized liquid extraction (PLE) to provide the highest yield of bioactive compounds. Different extraction solvents (distilled water, 50% and 96% ethanol) and different PLE parameters were investigated: temperature (40, 80, and 120 °C), static extraction time (5 and 10 min), and number of cycles (1 and 2). Total phenolic contents (TPC), hydroxycinnamic acids (HCA), flavonols (FL), total flavonoids (TF), condensed tannins (CT), and antioxidant capacity (DPPH and FRAP) were determined in PLE extracts. Solvent type, temperature, static extraction time, and number of cycles had a statistically significant effect on all bioactive compounds and antioxidant capacity (<i>p</i> ≤ 0.05). All bioactive compounds were positively correlated with temperature, except for TPC and DPPH. For all polyphenols studied, the optimal PLE parameters were a temperature of 120 °C, a static extraction time of 10 min and 2 cycles. The best solvent for most bioactive compounds was 96% ethanol, except for TPC, for which 50% ethanol was better. This study suggests that PLE is a promising tool to intensify the extraction of bioactive compounds from strawberry tree fruits. |
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| language | English |
| last_indexed | 2024-03-11T03:23:05Z |
| publishDate | 2023-05-01 |
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| series | Plants |
| spelling | doaj.art-180b47df6a23496e8298e3c70708a7232023-11-18T02:56:16ZengMDPI AGPlants2223-77472023-05-011210200610.3390/plants12102006Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.)Anica Bebek Markovinović0Sanja Milošević1Nemanja Teslić2Branimir Pavlić3Predrag Putnik4Irena Brčić Karačonji5Karlo Jurica6Dario Lasić7Danijela Bursać Kovačević8Faculty of Food Technology and of Biotechnology, University Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Technology, University of Novi Sad, Blvd. Cara Lazara 1, 21000 Novi Sad, SerbiaInstitute of Food Technology, University of Novi Sad, Blvd. Cara Lazara 1, 21000 Novi Sad, SerbiaFaculty of Technology, University of Novi Sad, Blvd. Cara Lazara 1, 21000 Novi Sad, SerbiaDepartment of Food Technology, University North, Trg dr. Žarka Dolinara 1, 48000 Koprivnica, CroatiaInstitute for Medical Research and Occupational Health, Ksaverska Cesta 2, 10000 Zagreb, CroatiaSpecial Security Operations Directorate, Ministry of the Interior, Ulica Grada Vukovara 33, 10000 Zagreb, CroatiaAndrija Štampar Teaching Institute for Public Health, Mirogojska 16, 10000 Zagreb, CroatiaFaculty of Food Technology and of Biotechnology, University Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaStrawberry tree fruits (<i>Arbutus unedo</i> L.) are a natural source of valuable biologically active compounds. Therefore, the aim of this work was to develop a green extraction approach using pressurized liquid extraction (PLE) to provide the highest yield of bioactive compounds. Different extraction solvents (distilled water, 50% and 96% ethanol) and different PLE parameters were investigated: temperature (40, 80, and 120 °C), static extraction time (5 and 10 min), and number of cycles (1 and 2). Total phenolic contents (TPC), hydroxycinnamic acids (HCA), flavonols (FL), total flavonoids (TF), condensed tannins (CT), and antioxidant capacity (DPPH and FRAP) were determined in PLE extracts. Solvent type, temperature, static extraction time, and number of cycles had a statistically significant effect on all bioactive compounds and antioxidant capacity (<i>p</i> ≤ 0.05). All bioactive compounds were positively correlated with temperature, except for TPC and DPPH. For all polyphenols studied, the optimal PLE parameters were a temperature of 120 °C, a static extraction time of 10 min and 2 cycles. The best solvent for most bioactive compounds was 96% ethanol, except for TPC, for which 50% ethanol was better. This study suggests that PLE is a promising tool to intensify the extraction of bioactive compounds from strawberry tree fruits.https://www.mdpi.com/2223-7747/12/10/2006strawberry tree fruitphenolic compoundsantioxidant capacitypressurized liquid extraction (PLE)green extractionoptimization |
| spellingShingle | Anica Bebek Markovinović Sanja Milošević Nemanja Teslić Branimir Pavlić Predrag Putnik Irena Brčić Karačonji Karlo Jurica Dario Lasić Danijela Bursać Kovačević Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.) Plants strawberry tree fruit phenolic compounds antioxidant capacity pressurized liquid extraction (PLE) green extraction optimization |
| title | Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.) |
| title_full | Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.) |
| title_fullStr | Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.) |
| title_full_unstemmed | Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.) |
| title_short | Development of a Pressurized Green Liquid Extraction Procedure to Recover Antioxidant Bioactive Compounds from Strawberry Tree Fruit (<i>Arbutus unedo</i> L.) |
| title_sort | development of a pressurized green liquid extraction procedure to recover antioxidant bioactive compounds from strawberry tree fruit i arbutus unedo i l |
| topic | strawberry tree fruit phenolic compounds antioxidant capacity pressurized liquid extraction (PLE) green extraction optimization |
| url | https://www.mdpi.com/2223-7747/12/10/2006 |
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