Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer
Macrophages play an important role in the suppression and activation of immune anti-cancer response, but little is known about dominant macrophage phenotype in the lung cancer environment, evaluated by bronchoalveolar lavage fluid (BALF). The aim of this study was to characterize macrophages in BALF...
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MDPI AG
2023-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/21/5175 |
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| author | Iwona Kwiecień Elżbieta Rutkowska Agata Raniszewska Agnieszka Rzeszotarska Małgorzata Polubiec-Kownacka Joanna Domagała-Kulawik Jolanta Korsak Piotr Rzepecki |
| author_facet | Iwona Kwiecień Elżbieta Rutkowska Agata Raniszewska Agnieszka Rzeszotarska Małgorzata Polubiec-Kownacka Joanna Domagała-Kulawik Jolanta Korsak Piotr Rzepecki |
| author_sort | Iwona Kwiecień |
| collection | DOAJ |
| description | Macrophages play an important role in the suppression and activation of immune anti-cancer response, but little is known about dominant macrophage phenotype in the lung cancer environment, evaluated by bronchoalveolar lavage fluid (BALF). The aim of this study was to characterize macrophages in BALF from a lung affected by cancer (cBALF) and a healthy lung (hBALF) of the same patient regarding their individual macrophage polarization and selected cytokines profile. A total of 36 patients with confirmed lung cancer were investigated. Macrophages markers: CD206 CD163 CD80 CD86 CD40 CD45, Arginase-1, and CD68 were evaluated by flow cytometry. Cytokines (IL-1 RA, IL-6, IL-10, TNF-α, IL-1β, IL-12, IL-23, and TGF-β) profile was analyzed. There was higher median proportion of macrophages in Cbalf than in Hbalf. The population of macrophages presented immunophenotype: Ccd68+<sup>bright</sup> CD206+<sup>bright</sup> CD163+<sup>bright</sup> CD80+ CD86+ CD40+<sup>bright</sup> CD45+ cArginase+. We observed some trends in the expression of the analyzed antigens in clBALF and hlBLAF. The highest concentrations of IL-1RA and IL-6 were in Cbalf and Hbalf supernatant. There were the correlations between pro- and anti-inflammatory cytokines. The findings showed that macrophages include a diverse and plastic group with the presence of different antigens and cytokines, and determining the target phenotype is a complex and variable process. |
| first_indexed | 2024-03-11T11:32:21Z |
| format | Article |
| id | doaj.art-180b4b5b379f4f16980e2df2669bb05d |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T11:32:21Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-180b4b5b379f4f16980e2df2669bb05d2023-11-10T15:00:08ZengMDPI AGCancers2072-66942023-10-011521517510.3390/cancers15215175Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung CancerIwona Kwiecień0Elżbieta Rutkowska1Agata Raniszewska2Agnieszka Rzeszotarska3Małgorzata Polubiec-Kownacka4Joanna Domagała-Kulawik5Jolanta Korsak6Piotr Rzepecki7Laboratory of Hematology and Flow Cytometry, Department of Internal Medicine and Hematology, Military Institute of Medicine-National Research Institute, Szaserów 128 Street, 04-141 Warsaw, PolandLaboratory of Hematology and Flow Cytometry, Department of Internal Medicine and Hematology, Military Institute of Medicine-National Research Institute, Szaserów 128 Street, 04-141 Warsaw, PolandLaboratory of Hematology and Flow Cytometry, Department of Internal Medicine and Hematology, Military Institute of Medicine-National Research Institute, Szaserów 128 Street, 04-141 Warsaw, PolandDepartment of Clinical Transfusion Medicine, Military Institute of Medicine-National Research Institute, Szaserów 128 Street, 04-141 Warsaw, PolandDepartment of Surgery, Institute of Tuberculosis and Lung Diseases, Płocka 26 Street, 01-138 Warsaw, PolandInstitute of Clinical Sciences, Maria Curie-Sklodowska Medical Academy, 03-411 Warsaw, PolandDepartment of Clinical Transfusion Medicine, Military Institute of Medicine-National Research Institute, Szaserów 128 Street, 04-141 Warsaw, PolandDepartment of Internal Medicine and Hematology, Military Institute of Medicine-National Research Institute, Szaserów 128 Street, 04-141 Warsaw, PolandMacrophages play an important role in the suppression and activation of immune anti-cancer response, but little is known about dominant macrophage phenotype in the lung cancer environment, evaluated by bronchoalveolar lavage fluid (BALF). The aim of this study was to characterize macrophages in BALF from a lung affected by cancer (cBALF) and a healthy lung (hBALF) of the same patient regarding their individual macrophage polarization and selected cytokines profile. A total of 36 patients with confirmed lung cancer were investigated. Macrophages markers: CD206 CD163 CD80 CD86 CD40 CD45, Arginase-1, and CD68 were evaluated by flow cytometry. Cytokines (IL-1 RA, IL-6, IL-10, TNF-α, IL-1β, IL-12, IL-23, and TGF-β) profile was analyzed. There was higher median proportion of macrophages in Cbalf than in Hbalf. The population of macrophages presented immunophenotype: Ccd68+<sup>bright</sup> CD206+<sup>bright</sup> CD163+<sup>bright</sup> CD80+ CD86+ CD40+<sup>bright</sup> CD45+ cArginase+. We observed some trends in the expression of the analyzed antigens in clBALF and hlBLAF. The highest concentrations of IL-1RA and IL-6 were in Cbalf and Hbalf supernatant. There were the correlations between pro- and anti-inflammatory cytokines. The findings showed that macrophages include a diverse and plastic group with the presence of different antigens and cytokines, and determining the target phenotype is a complex and variable process.https://www.mdpi.com/2072-6694/15/21/5175macrophagescytokines profilebronchoalveolar lavage fluidCD68CD163M1 |
| spellingShingle | Iwona Kwiecień Elżbieta Rutkowska Agata Raniszewska Agnieszka Rzeszotarska Małgorzata Polubiec-Kownacka Joanna Domagała-Kulawik Jolanta Korsak Piotr Rzepecki Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer Cancers macrophages cytokines profile bronchoalveolar lavage fluid CD68 CD163 M1 |
| title | Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer |
| title_full | Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer |
| title_fullStr | Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer |
| title_full_unstemmed | Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer |
| title_short | Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer |
| title_sort | flow cytometric analysis of macrophages and cytokines profile in the bronchoalveolar lavage fluid in patients with lung cancer |
| topic | macrophages cytokines profile bronchoalveolar lavage fluid CD68 CD163 M1 |
| url | https://www.mdpi.com/2072-6694/15/21/5175 |
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