Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4519282?pdf=render |
| _version_ | 1829115428265263104 |
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| author | Jeffrey M B Musser J Jill Heatley Anastasia V Koinis Paulette F Suchodolski Jianhua Guo Paulina Escandon Ian R Tizard |
| author_facet | Jeffrey M B Musser J Jill Heatley Anastasia V Koinis Paulette F Suchodolski Jianhua Guo Paulina Escandon Ian R Tizard |
| author_sort | Jeffrey M B Musser |
| collection | DOAJ |
| description | Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture. |
| first_indexed | 2024-12-12T16:15:34Z |
| format | Article |
| id | doaj.art-180e18a9151546119a1096fde36ec9d6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T16:15:34Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-180e18a9151546119a1096fde36ec9d62022-12-22T00:19:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013408010.1371/journal.pone.0134080Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.Jeffrey M B MusserJ Jill HeatleyAnastasia V KoinisPaulette F SuchodolskiJianhua GuoPaulina EscandonIan R TizardParrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.http://europepmc.org/articles/PMC4519282?pdf=render |
| spellingShingle | Jeffrey M B Musser J Jill Heatley Anastasia V Koinis Paulette F Suchodolski Jianhua Guo Paulina Escandon Ian R Tizard Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture. PLoS ONE |
| title | Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture. |
| title_full | Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture. |
| title_fullStr | Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture. |
| title_full_unstemmed | Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture. |
| title_short | Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture. |
| title_sort | ribavirin inhibits parrot bornavirus 4 replication in cell culture |
| url | http://europepmc.org/articles/PMC4519282?pdf=render |
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