Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice
Purpose Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigate...
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| Format: | Article |
| Language: | English |
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Korean Continence Society
2018-10-01
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| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-1836228-114.pdf |
| _version_ | 1818205282984001536 |
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| author | Eun-Jin Moon Il-Gyu Ko Sung-Eun Kim Jun-Jang Jin Lakkyong Hwang Chang-Ju Kim Hyeonjun An Bong-Jae Lee Jae-Woo Yi |
| author_facet | Eun-Jin Moon Il-Gyu Ko Sung-Eun Kim Jun-Jang Jin Lakkyong Hwang Chang-Ju Kim Hyeonjun An Bong-Jae Lee Jae-Woo Yi |
| author_sort | Eun-Jin Moon |
| collection | DOAJ |
| description | Purpose Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice. Methods For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 μg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed. Results Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment. Conclusions In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor. |
| first_indexed | 2024-12-12T03:54:40Z |
| format | Article |
| id | doaj.art-180f3d32c0ef4866bad02bffb7e839d1 |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-12-12T03:54:40Z |
| publishDate | 2018-10-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-180f3d32c0ef4866bad02bffb7e839d12022-12-22T00:39:17ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312018-10-0122Suppl 3S13914610.5213/inj.1836228.114755Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in MiceEun-Jin Moon0Il-Gyu Ko1Sung-Eun Kim2Jun-Jang Jin3Lakkyong Hwang4Chang-Ju Kim5Hyeonjun An6Bong-Jae Lee7Jae-Woo Yi8 Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, KoreaPurpose Sleep deprivation induces depressive symptoms. Dexmedetomidine is a α2-adrenoreceptor agonist and this drug possesses sedative, anxiolytic, analgesic, and anesthetic-sparing effect. In this study, the action of dexmedetomidine on sleep deprivation-induced depressive behaviors was investigated using mice. Methods For the inducing of sleep deprivation, the mice were placed inside a water cage containing 15 platforms and filled with water up to 1 cm below the platform surface for 7 days. One day after sleep deprivation, dexmedetomidine at the respective dosage (0.5, 1, and 2 μg/kg) was intraperitoneally treated into the mice, one time per a day during 6 days. Then, forced swimming test and tail suspension test were conducted. Immunohistochemistry for tyrosine hydroxylase (TH), 5-hydroxytryptamine (5-HT; serotonin), tryptophan hydroxylase (TPH) and western blot for D1 dopamine receptor were also performed. Results Sleep deprivation increased the immobility latency in the forced swimming test and tail suspension test. The expressions of TPH, 5-HT, and D1 dopamine receptor were decreased, whereas, TH expression was increased by sleep deprivation. Dexmedetomidine decreased the immobility latency and increased the expressions of TPH, 5-HT, and D1 dopamine receptor, whereas, HT expression was decreased by dexmedetomidine treatment. Conclusions In our results, dexmedetomidine alleviated sleep deprivation-induced depressive behaviors by increasing 5-HT synthesis and by decreasing dopamine production with up-regulation of D1 dopamine receptor.http://www.einj.org/upload/pdf/inj-1836228-114.pdfSleep deprivationDexmedetomidineDepressionSerotoninDopamine |
| spellingShingle | Eun-Jin Moon Il-Gyu Ko Sung-Eun Kim Jun-Jang Jin Lakkyong Hwang Chang-Ju Kim Hyeonjun An Bong-Jae Lee Jae-Woo Yi Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice International Neurourology Journal Sleep deprivation Dexmedetomidine Depression Serotonin Dopamine |
| title | Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice |
| title_full | Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice |
| title_fullStr | Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice |
| title_full_unstemmed | Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice |
| title_short | Dexmedetomidine Ameliorates Sleep Deprivation-Induced Depressive Behaviors in Mice |
| title_sort | dexmedetomidine ameliorates sleep deprivation induced depressive behaviors in mice |
| topic | Sleep deprivation Dexmedetomidine Depression Serotonin Dopamine |
| url | http://www.einj.org/upload/pdf/inj-1836228-114.pdf |
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