Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study
Abstract Background Serum biochemical changes during laparoscopic surgery and positive pressure pneumoperitoneum (PP) may reflect mild oxidative stress due to the ischemia-reperfusion (I/R) mechanism. However, there is still a controversy regarding the exact mechanism of PP in creating oxidative str...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Surgery |
| Online Access: | https://doi.org/10.1186/s12893-021-01339-4 |
| _version_ | 1818442371934715904 |
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| author | Amitai Bickel Soliman Khatib Eli Kakiashvilli Eilam Palzur |
| author_facet | Amitai Bickel Soliman Khatib Eli Kakiashvilli Eilam Palzur |
| author_sort | Amitai Bickel |
| collection | DOAJ |
| description | Abstract Background Serum biochemical changes during laparoscopic surgery and positive pressure pneumoperitoneum (PP) may reflect mild oxidative stress due to the ischemia-reperfusion (I/R) mechanism. However, there is still a controversy regarding the exact mechanism of PP in creating oxidative stress and whether the induction of PP causes I/R effects at all. To elucidate this debated issue, we studied, for the first time, the changes of I/R parameters in the serum, in a pilot study, during laparoscopic cholecystectomy using a reliable, independent exogenous oxidative biomarker, together with common intrinsic biomarkers of oxidative stress. Patients and methods Our study included 20 patients scheduled for elective laparoscopic cholecystectomy. We evaluated the levels of the extrinsic and endogenous markers for oxidative stress during awareness, under anesthesia, the end of surgery (abdominal CO2 evacuation), and 2 h afterward. Results After an initial increase in oxidative stress following anesthesia, we did not notice any further significant rise in the levels of the synthetic exogenous and the endogenous biomarkers at the end of the surgery and 2 h later on. However, a positive correlation was noted between the levels of both the intrinsic and extrinsic markers. Conclusions In our study, the capability of the extrinsic biomarker to detect mild oxidative stress was not validated. Our study stresses the heterogeneous nature of the oxidative reactions and the diversity of the endogenous and exogenous biomarkers while detecting various biochemical patterns under mild oxidative stress, during the short period of laparoscopic surgery. |
| first_indexed | 2024-12-14T18:43:05Z |
| format | Article |
| id | doaj.art-18101002f4204f628bd3d7da870aae6a |
| institution | Directory Open Access Journal |
| issn | 1471-2482 |
| language | English |
| last_indexed | 2024-12-14T18:43:05Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Surgery |
| spelling | doaj.art-18101002f4204f628bd3d7da870aae6a2022-12-21T22:51:27ZengBMCBMC Surgery1471-24822021-09-012111810.1186/s12893-021-01339-4Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot studyAmitai Bickel0Soliman Khatib1Eli Kakiashvilli2Eilam Palzur3Department of Surgery A, Galilee Medical CenterDepartment of Biochemistry, MIGAL Galilee Research InstituteDepartment of Surgery A, Galilee Medical CenterEliachar Research Laboratories, Galilee Medical CenterAbstract Background Serum biochemical changes during laparoscopic surgery and positive pressure pneumoperitoneum (PP) may reflect mild oxidative stress due to the ischemia-reperfusion (I/R) mechanism. However, there is still a controversy regarding the exact mechanism of PP in creating oxidative stress and whether the induction of PP causes I/R effects at all. To elucidate this debated issue, we studied, for the first time, the changes of I/R parameters in the serum, in a pilot study, during laparoscopic cholecystectomy using a reliable, independent exogenous oxidative biomarker, together with common intrinsic biomarkers of oxidative stress. Patients and methods Our study included 20 patients scheduled for elective laparoscopic cholecystectomy. We evaluated the levels of the extrinsic and endogenous markers for oxidative stress during awareness, under anesthesia, the end of surgery (abdominal CO2 evacuation), and 2 h afterward. Results After an initial increase in oxidative stress following anesthesia, we did not notice any further significant rise in the levels of the synthetic exogenous and the endogenous biomarkers at the end of the surgery and 2 h later on. However, a positive correlation was noted between the levels of both the intrinsic and extrinsic markers. Conclusions In our study, the capability of the extrinsic biomarker to detect mild oxidative stress was not validated. Our study stresses the heterogeneous nature of the oxidative reactions and the diversity of the endogenous and exogenous biomarkers while detecting various biochemical patterns under mild oxidative stress, during the short period of laparoscopic surgery.https://doi.org/10.1186/s12893-021-01339-4 |
| spellingShingle | Amitai Bickel Soliman Khatib Eli Kakiashvilli Eilam Palzur Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study BMC Surgery |
| title | Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study |
| title_full | Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study |
| title_fullStr | Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study |
| title_full_unstemmed | Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study |
| title_short | Reappraisal of ischemia-reperfusion injury in a short duration laparoscopic surgery, a pilot study |
| title_sort | reappraisal of ischemia reperfusion injury in a short duration laparoscopic surgery a pilot study |
| url | https://doi.org/10.1186/s12893-021-01339-4 |
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