Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion
Objective: To explore the clinical significance of the quantitative detection of human papillomavirus (HPV) E6/E7mRNA in triage of patients with atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL). Methods: A cross-sectional screening st...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2018-01-01
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| Series: | Reproductive and Developmental Medicine |
| Subjects: | |
| Online Access: | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2018;volume=2;issue=3;spage=157;epage=161;aulast=Wang |
| _version_ | 1818237747297517568 |
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| author | Qing Wang Cai-Ying Zhu Li-Mei Chen Shu-Jun Gao Ming Du Hong-Wei Zhang Hua Feng Yu Song Wen-Jing Diao Yan-Yun Li Long Sui |
| author_facet | Qing Wang Cai-Ying Zhu Li-Mei Chen Shu-Jun Gao Ming Du Hong-Wei Zhang Hua Feng Yu Song Wen-Jing Diao Yan-Yun Li Long Sui |
| author_sort | Qing Wang |
| collection | DOAJ |
| description | Objective: To explore the clinical significance of the quantitative detection of human papillomavirus (HPV) E6/E7mRNA in triage of patients with atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL).
Methods: A cross-sectional screening study was conducted among women who underwent outpatient gynecological screening at the Obstetrics and Gynecology Hospital of Fudan University from September 2015 to July 2016. A total of 500 patients from our hospital with ASC-US or LSIL based on cytology testing were subjected to HPV DNA and HPV E6/E7 mRNA quantitative analysis.
Results: The specificity of the HPV E6/E7 mRNA test for detecting ≥ high-grade squamous intraepithelial lesion (HSIL+) was statistically higher than that of the HPV DNA test (61.3% vs. 40.0%, P < 0.05), whereas there was no significant difference in the sensitivity of HPV E6/E7 mRNA test and HPV DNA test (90.0% vs. 95.0%, P > 0.05). The positive rates of HPV in the participants tested by HPV E6/E7 mRNA and HPV DNA were, respectively, 42.8% (214/500) and 62.8% (314/500), with statistical significance (P < 0.05).
Conclusions: The HPV E6/E7 mRNA test was slightly less sensitivity than that of the HPV DNA test for diagnosing HSIL+ in patients with ASC-US and LSIL, but the difference was not significant, although the specificity of the former was significantly higher. HPV E6/E7 mRNA detection can effectively reduce overdiagnosis and overtreatment of patients with ASC-US and LSIL and has important clinical value in triage of patients with ASC-US and LSIL. |
| first_indexed | 2024-12-12T12:30:40Z |
| format | Article |
| id | doaj.art-1813f66853524a3d828d3b19cad6a32d |
| institution | Directory Open Access Journal |
| issn | 2096-2924 |
| language | English |
| last_indexed | 2024-12-12T12:30:40Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Reproductive and Developmental Medicine |
| spelling | doaj.art-1813f66853524a3d828d3b19cad6a32d2022-12-22T00:24:25ZengWolters Kluwer Health/LWWReproductive and Developmental Medicine2096-29242018-01-012315716110.4103/2096-2924.248485Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesionQing WangCai-Ying ZhuLi-Mei ChenShu-Jun GaoMing DuHong-Wei ZhangHua FengYu SongWen-Jing DiaoYan-Yun LiLong SuiObjective: To explore the clinical significance of the quantitative detection of human papillomavirus (HPV) E6/E7mRNA in triage of patients with atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL). Methods: A cross-sectional screening study was conducted among women who underwent outpatient gynecological screening at the Obstetrics and Gynecology Hospital of Fudan University from September 2015 to July 2016. A total of 500 patients from our hospital with ASC-US or LSIL based on cytology testing were subjected to HPV DNA and HPV E6/E7 mRNA quantitative analysis. Results: The specificity of the HPV E6/E7 mRNA test for detecting ≥ high-grade squamous intraepithelial lesion (HSIL+) was statistically higher than that of the HPV DNA test (61.3% vs. 40.0%, P < 0.05), whereas there was no significant difference in the sensitivity of HPV E6/E7 mRNA test and HPV DNA test (90.0% vs. 95.0%, P > 0.05). The positive rates of HPV in the participants tested by HPV E6/E7 mRNA and HPV DNA were, respectively, 42.8% (214/500) and 62.8% (314/500), with statistical significance (P < 0.05). Conclusions: The HPV E6/E7 mRNA test was slightly less sensitivity than that of the HPV DNA test for diagnosing HSIL+ in patients with ASC-US and LSIL, but the difference was not significant, although the specificity of the former was significantly higher. HPV E6/E7 mRNA detection can effectively reduce overdiagnosis and overtreatment of patients with ASC-US and LSIL and has important clinical value in triage of patients with ASC-US and LSIL.http://www.repdevmed.org/article.asp?issn=2096-2924;year=2018;volume=2;issue=3;spage=157;epage=161;aulast=WangAtypical Squamous Cells of Undetermined Significance; Human Papillomavirus; Human Papillomavirus E6/E7 mRNA; Low-Grade Squamous Intraepithelial Lesion |
| spellingShingle | Qing Wang Cai-Ying Zhu Li-Mei Chen Shu-Jun Gao Ming Du Hong-Wei Zhang Hua Feng Yu Song Wen-Jing Diao Yan-Yun Li Long Sui Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion Reproductive and Developmental Medicine Atypical Squamous Cells of Undetermined Significance; Human Papillomavirus; Human Papillomavirus E6/E7 mRNA; Low-Grade Squamous Intraepithelial Lesion |
| title | Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion |
| title_full | Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion |
| title_fullStr | Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion |
| title_full_unstemmed | Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion |
| title_short | Clinical value of human papillomavirus E6/E7 mRNA testing in patients with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion |
| title_sort | clinical value of human papillomavirus e6 e7 mrna testing in patients with atypical squamous cells of undetermined significance and low grade squamous intraepithelial lesion |
| topic | Atypical Squamous Cells of Undetermined Significance; Human Papillomavirus; Human Papillomavirus E6/E7 mRNA; Low-Grade Squamous Intraepithelial Lesion |
| url | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2018;volume=2;issue=3;spage=157;epage=161;aulast=Wang |
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